Vimentin Intermediate Filaments Undergo Irreversible Conformational Changes during Cyclic Loading

• Published in: Nano letters Vol. 19; no. 10; pp. 7349 - 7356
• Main Authors: Forsting, Johanna, Kraxner, Julia, Witt, Hannes, Janshoff, Andreas, Köster, Sarah
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 09.10.2019
• Subjects: optical tweezers; force-strain behavior; cytoskeleton; cell mechanics; intermediate filaments; 3-state system
• ISSN: 1530-6984; 1530-6992; 1530-6992
• DOI: 10.1021/acs.nanolett.9b02972

Intermediate filaments (IFs) are part of the cytoskeleton of eukaryotic cells and, therefore, are largely responsible for the cell’s mechanical properties. IFs are characterized by a pronounced extensibility and remarkable resilience that enable them to support cells in extreme situations. Previous experiments showed that, under strain, α-helices in vimentin IFs might unfold to β-sheets. Upon repeated stretching, the filaments soften; however, the remaining plastic strain is negligible. Here, we observe that vimentin IFs do not recover their original stiffness on reasonable time scales, and we explain these seemingly contradicting results by introducing a third, less well-defined conformational state. Reversibility on the nanoscale can be fully rescued by introducing cross-linkers that prevent transition to the β-sheet. Our results classify IFs as a nanomaterial with intriguing mechanical properties, which is likely to play a major role for the cell’s local adaption to external stimuli.