Short Hexapeptide Optimized from Rice-Derived Peptide 1 Shows Promising Anti-hyperuricemia Activities

• Published in: Journal of agricultural and food chemistry Vol. 70; no. 22; pp. 6679 - 6687
• Main Authors: Shu, Longjun, Yang, Meifeng, Liu, Naixin, Liu, Yixiang, Sun, Huiling, Wang, Siyu, Zhang, Yue, Li, Yilin, Yang, Xinwang, Wang, Ying
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 08.06.2022
• Subjects: Bioactive Constituents, Metabolites, and Functions; drugs; food chemistry; glucose transporters; inflammasomes; necrosis; neoplasms; rice; uric acid; xanthine oxidase; hyperuricemia; peptide; RDP1; uric acid
• ISSN: 0021-8561; 1520-5118; 1520-5118
• DOI: 10.1021/acs.jafc.2c00354

Plant-derived peptides are a treasure trove for new-generation anti-hyperuricemia drugs. In the current study, we optimized a short hexapeptide rice-derived peptide 1 (RDP1)-M3 (AAAAGA) according to the anti-hyperuricemia RDP1 peptide identified from rice in our previous research. Results showed that RDP1-M3 exerted better hyperuricemia-alleviating and xanthine oxidase (XOD)-inhibiting potency in mice than RDP1. The biodistribution of RDP1-M3 was also analyzed. RDP1-M3 directly decreased XOD and uric acid levels in vivo and in vitro. In addition, RDP1-M3 reduced the expression of urate transporter 1 and glucose transporter 9, increased the level of organic anion transporter 1, reduced the expression of NOD-like receptor superfamily pyrin 3 inflammasomes, and reduced the levels of interleukin-1β and tumor necrosis factor-α of hyperuricemic mice. Thus, our results indicated that the optimized short hexapeptide RDP1-M3 may be a candidate drug for anti-hyperuricemia.