Ultrasensitive Detection of the Hepatitis E Virus by Electrocatalytic Water Oxidation Using Pt-Co3O4 Hollow Cages

• Published in: ACS applied materials & interfaces Vol. 12; no. 45; pp. 50212 - 50221
• Main Authors: Ganganboina, Akhilesh Babu, Khoris, Indra Memdi, Chowdhury, Ankan Dutta, Li, Tian-Cheng, Park, Enoch Y
• Format: Journal Article
• Language: English
• Published: American Chemical Society 11.11.2020
• Subjects: antibodies; Biological and Medical Applications of Materials and Interfaces; blood serum; cell culture; cobalt; detection limit; imidazole; immunoassays; magnetism; Orthohepevirus A; oxidation; platinum; probability; rapid methods; RNA; synergism; viruses; voltammetry; zeolites; platinum; hepatitis E virus; electrocatalytic nanoparticles; hollow cobalt oxide; magnetic nanoparticles; water oxidation reaction
• ISSN: 1944-8244; 1944-8252; 1944-8252
• DOI: 10.1021/acsami.0c13247

A sensitive virus detection method applicable for an early stage increases the probability of survival. Here, we develop a simple and rapid detection strategy for the detection of the hepatitis E virus (HEV) by an electrocatalytic water oxidation reaction (WOR) using a platinum (Pt)-incorporated cobalt (Co)-based zeolite imidazole framework (ZIF-67). The surface cavity of ZIF-67 enables the rich loading of Pt NPs, and subsequent calcination etches the cavity, promoting the electrocatalytic activity of Pt-Co3O4 HCs. The Pt-Co3O4 HCs show excellent behavior for the WOR due to the synergistic interaction of Pt and Co3O4, evaluated by voltammetry and chronoamperometry. The synthesized Pt-Co3O4 HCs are conjugated with anti-HEV antibody (Ab@Pt-Co3O4 HCs); the electrocatalytic activity of Ab@Pt-Co3O4 HCs is combined with that of antibody-conjugated magnetic nanoparticles (MNPs) for HEV detection by a magneto-and-nanocomposite sandwich immunoassay. The sensor is challenged to detect the HEV in spiked serum samples and HEV G7 genotypes collected from the cell culture supernatant, reaching a low limit of detection down to 61 RNA copies mL–1. This work establishes a free-indicator one-step approach with the controlled design of Pt-Co3O4 HCs, which presents an effective WOR technique for virus detection in a neutral pH solution, which can be extended to electrocatalytic studies in the future integrated biosensing systems.