2-DE and LC-MS/MS for a Comparative Proteomic Analysis of BALf from Subjects with Different Subsets of Inflammatory Myopathies

• Published in: Journal of proteome research Vol. 8; no. 5; pp. 2331 - 2340
• Main Authors: Passadore, Ileana, Iadarola, Paolo, Di Poto, Cristina, Giuliano, Serena, Montecucco, Carlomaurizio, Cavagna, Lorenzo, Bonino, Claudia, Meloni, Federica, Fietta, Anna Maria, Lisa, Antonella, Salvini, Roberta, Bardoni, Anna Maria
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01.05.2009
• Subjects: Aged; Bronchoalveolar Lavage Fluid - chemistry; Chromatography, Liquid; Cofilin 1 - blood; Dermatomyositis - blood; Dermatomyositis - metabolism; Dermatomyositis - pathology; Electrophoresis, Gel, Two-Dimensional - methods; Gelsolin - blood; Humans; Immunoblotting; Middle Aged; Proteins - analysis; Proteins - classification; Proteins - metabolism; Proteomics - methods; Reproducibility of Results; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Tandem Mass Spectrometry; Vimentin - blood; PM/DM; LC-MS/MS; AS; Overlap Syndrome; 2-DE
• ISSN: 1535-3893; 1535-3907
• DOI: 10.1021/pr800943t

The protein profiles of bronchoalveolar lavage fluid (BALf) of patients belonging to three selected subsets of Polymyositis/Dermatomyositis (PM/DM) have been compared by using a combination of 2-DE and MALDI-TOF/MS or LC-MS/MS. Our study examined the hypothesis that there were distinct differences in protein expression profiles that were related to the phenotype. From among the 323 ± 51 protein spots that may represent the most highly expressed proteins in BALf of these patients, 24 unique spots were isolated and proteins identified. In particular, 9 spots were present in BALf of PM/DM patients only; 12 spots were exclusive of Overlap patients and 3 spots of AS patients. From among the proteins identified, a few were classified as cytoskeletal proteins, others were involved in oxidative stress and a number of proteins were associated with general metabolic activity or immunological response and inflammation. This is the first study in which evidence is provided that a number of different proteins are expressed in different subsets of PM/DM and supports our contention that the proteomic approach would be beneficial in discovering molecules which could represent possible prognostic factors of these rare pathologies.