Conversion of Phenolic Acids in Canola Fermentation: Impact on Antimicrobial Activity against Salmonella enterica and Campylobacter jejuni

• Published in: Journal of agricultural and food chemistry Vol. 71; no. 4; pp. 2059 - 2069
• Main Authors: Pham, Vi D., Korver, Douglas R., Gänzle, Michael G.
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01.02.2023
• Subjects: Anti-Infective Agents - metabolism; antimicrobial properties; Biotechnology and Biological Transformations; Campylobacter jejuni; canola; canola meal; Chromatography, Liquid; Coumaric Acids - metabolism; fermentation; food chemistry; glucosides; high performance liquid chromatography; lactic acid; Lactobacillus; Lactobacillus - metabolism; minimum inhibitory concentration; Phenols - metabolism; poultry; protein content; Salmonella enterica; Salmonella enterica - metabolism; sinapic acid; Tandem Mass Spectrometry; antimicrobial compounds; polyphenols; Salmonella; poultry; canola meal; phenolic acids; Lactobacillus
• ISSN: 0021-8561; 1520-5118; 1520-5118
• DOI: 10.1021/acs.jafc.2c08322

Canola meal (CM) is commonly used in poultry feeds. CM has a high protein content but also contains high levels of antimicrobial phenolic acids. Lactic acid bacteria can alter CM phenolic composition during fermentation and influence its antimicrobial activity against pathogens. Fermented CM was analyzed for phenolic composition using tandem mass spectrometry (LC–MS/MS) and high-performance liquid chromatography (HPLC). Sinapic acid and derivatives were the major phenolic acids in CM. Growth of lactobacilli in CM was attenuated when compared to cereal substrates. Glucosides and esters of sinapic acid were extensively hydrolyzed during fermentation with Lactiplantibacillus plantarum and Furfurilactobacillus milii. Lp. plantarum transformed hydroxycinnamic acids to dihydro, 4-vinyl, and 4-ethyl derivatives, Ff. milii reduced hydroxycinnamic acids to dihydroderivatives, but Limosilactobacillus reuteri did not convert hydroxycinnamic acids. The minimum inhibitory concentration of phenolic extracts was assessed with lactobacilli, Salmonella, and Campylobacter jejuni as indicator strains. Fermentation of CM with Lp. plantarum or Ff. milii increased the antimicrobial activity of phenolic extracts against Salmonella enterica and Campylobacter jejuni. Fermentation with Lm. reuteri TMW1.656 but not fermentation with Lm. reuteri TMW1.656ΔrtcN increased the antimicrobial activity of extracts owing to the production of reutericyclin. This study demonstrates that fermentation of CM with lactobacilli converts hydroxycinammic esters and may increase the antimicrobial activity of phenolic compounds in CM against pathogens.