Effects of Copper and Cadmium Exposure on mRNA Expression of Catalase, Glutamine Synthetase, Cytochrome P450 and Heat Shock Protein 70 in Tambaqui Fish (Colossoma Macropomum)

• Published in: Gene Expression to Genetical Genomics Vol. 6; no. 2013; pp. 1 - 8
• Main Authors: Casanova, Frida Mesel, Honda, Rubens Tomio, Ferreira-Nozawa, Monica Stropa, Aride, Paulo Henrique Rocha, Nozawa, Sergio Ricardo
• Format: Journal Article
• Language: English
• Published: London Libertas Academica 19.02.2013; Sage Publications Ltd. (UK); Sage Publications Ltd
• Subjects: Actinopterygii; Cadmium; Copper; Gene expression; Genetic aspects; Genetic research; Identification and classification; Properties; RNA; Colossoma macropomum; hsp70; Amazon; antioxidants; copper and cadmium exposure
• ISSN: 1179-5697; 1179-5697
• DOI: 10.4137/GGG.S10716

In this work the authors evaluated the expression of cat, glu, cypP450, hsp70 and 18S RNA in Colossoma macropomum following exposure to copper and cadmium by semi-quantitative RT-PCR and quantitative qRT-PCR. In RT-PCR and qRT-PCR hsp70 expression was highest after exposure to copper for 3 hours (h). glu expression increased after exposure to cadmium, regardless of time. cat expression increased after the first hour of exposure to both metals, and decreased after 3 hs. cyp450 expression was higher when compared to controls in exposed fish regardless of metal and exposure time. cyp450 expression after exposure to copper for 3 h was twice that recorded after 1 h. In the presence of cadmium, cyp450 expression was higher in the animals exposed for 1 h and lower at 3 h. Therefore, toxicology tests and biomonitoring based on molecular markers should focus on a set of molecular and/or environmental parameters, rather than isolated markers.