麦粒灸抑制5XFAD转基因模型小鼠阿尔茨海默病的发展
R2-03; 目的:利用可以共同表达5个家族性阿尔茨海默病(AD)基因突变(5XFAD)的转基因小鼠,探讨在AD病理早期进行心俞和肾俞麦粒灸法是否可以改善AD小鼠认知功能下降及其他病理特征.方法:采用聚合酶链反应法鉴定转基因小鼠的基因表型.将40只1.5月龄的转基因小鼠随机等分为AD模型组(5XFAD组)和麦粒灸治疗组(5XFAD+GM组),20只C57BL/6J野生型小鼠为正常对照组(WT组).5XFAD+GM组小鼠取双侧心俞、肾俞行麦粒灸治疗.WT组和5XFAD组小鼠不接受治疗仅作固定,实验环境和5XFAD+GM组一致.应用Morris水迷宫和Y迷宫评价小鼠认知和记忆能力.应用酶联免疫吸附...
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| Published in | 针灸推拿医学（英文版） Vol. 20; no. 6; pp. 419 - 432 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
2022
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1672-3597 |
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| Abstract | R2-03; 目的:利用可以共同表达5个家族性阿尔茨海默病(AD)基因突变(5XFAD)的转基因小鼠,探讨在AD病理早期进行心俞和肾俞麦粒灸法是否可以改善AD小鼠认知功能下降及其他病理特征.方法:采用聚合酶链反应法鉴定转基因小鼠的基因表型.将40只1.5月龄的转基因小鼠随机等分为AD模型组(5XFAD组)和麦粒灸治疗组(5XFAD+GM组),20只C57BL/6J野生型小鼠为正常对照组(WT组).5XFAD+GM组小鼠取双侧心俞、肾俞行麦粒灸治疗.WT组和5XFAD组小鼠不接受治疗仅作固定,实验环境和5XFAD+GM组一致.应用Morris水迷宫和Y迷宫评价小鼠认知和记忆能力.应用酶联免疫吸附法检测小鼠脑组织β淀粉样蛋白40(Aβ40)和β淀粉样蛋白42(Aβ42)水平;采用硫黄素-S染色检测小鼠脑组织中淀粉样斑块水平;采用免疫组化法检测小鼠海马区和额叶皮层胶质纤维酸性蛋白(GFAP)、分化簇11b(CD11b)、脑源性神经营养因子(BDNF)及胆碱乙酰转移酶(ChAT)的表达水平.结果:在Morris水迷宫行为学测试中,与5XFAD组比较,5XFAD+GM组逃避潜伏期缩短,穿越平台次数增多,目标象限停留时间百分比增加(P<0.05).Y-迷宫实验中,与5XFAD组比较,5XFAD+GM组达标所需电击次数缩短(P<0.05),正确反应次数增加(P<0.05).与5XFAD组比较,5XFAD+GM组小鼠脑内Aβ40及Aβ42水平降低(P<0.05);海马区及额叶皮层β淀粉样斑块数目减少(P<0.05),GFAP及CD11b表达水平下降(P<0.05),ChAT及BDNF表达水平升高(P<0.05).结论:麦粒灸心俞和肾俞可以提高AD小鼠的学习记忆能力,下调小鼠脑内Aβ40和Aβ42水平,抑制β淀粉样斑块的沉积,减少GFAP及CD11b的表达,增加BDNF及ChAT的表达,从而抑制AD的进展. |
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| AbstractList | R2-03; 目的:利用可以共同表达5个家族性阿尔茨海默病(AD)基因突变(5XFAD)的转基因小鼠,探讨在AD病理早期进行心俞和肾俞麦粒灸法是否可以改善AD小鼠认知功能下降及其他病理特征.方法:采用聚合酶链反应法鉴定转基因小鼠的基因表型.将40只1.5月龄的转基因小鼠随机等分为AD模型组(5XFAD组)和麦粒灸治疗组(5XFAD+GM组),20只C57BL/6J野生型小鼠为正常对照组(WT组).5XFAD+GM组小鼠取双侧心俞、肾俞行麦粒灸治疗.WT组和5XFAD组小鼠不接受治疗仅作固定,实验环境和5XFAD+GM组一致.应用Morris水迷宫和Y迷宫评价小鼠认知和记忆能力.应用酶联免疫吸附法检测小鼠脑组织β淀粉样蛋白40(Aβ40)和β淀粉样蛋白42(Aβ42)水平;采用硫黄素-S染色检测小鼠脑组织中淀粉样斑块水平;采用免疫组化法检测小鼠海马区和额叶皮层胶质纤维酸性蛋白(GFAP)、分化簇11b(CD11b)、脑源性神经营养因子(BDNF)及胆碱乙酰转移酶(ChAT)的表达水平.结果:在Morris水迷宫行为学测试中,与5XFAD组比较,5XFAD+GM组逃避潜伏期缩短,穿越平台次数增多,目标象限停留时间百分比增加(P<0.05).Y-迷宫实验中,与5XFAD组比较,5XFAD+GM组达标所需电击次数缩短(P<0.05),正确反应次数增加(P<0.05).与5XFAD组比较,5XFAD+GM组小鼠脑内Aβ40及Aβ42水平降低(P<0.05);海马区及额叶皮层β淀粉样斑块数目减少(P<0.05),GFAP及CD11b表达水平下降(P<0.05),ChAT及BDNF表达水平升高(P<0.05).结论:麦粒灸心俞和肾俞可以提高AD小鼠的学习记忆能力,下调小鼠脑内Aβ40和Aβ42水平,抑制β淀粉样斑块的沉积,减少GFAP及CD11b的表达,增加BDNF及ChAT的表达,从而抑制AD的进展. |
| Abstract_FL | Objective: To investigate whether grain-sized moxibustion at Xinshu (BL15) and Shenshu (BL23) can alleviate cognitive decline and other pathologic features in early-stage Alzheimer disease (AD) using transgenic mice with 5 familial AD mutations (5XFAD). Methods: The genotype of transgenic mice was detected by polymerase chain reaction. A total of 40 transgenic mice (1.5 months old) were randomly and equally allocated to an AD model group (5XFAD group) or a grain-sized moxibustion group (5XFAD + GM group), with 20 wild-type (WT) mice (C57BL/6J) serving as the normal control group (WT group). Mice in the 5XFAD + GM group were treated by grain-sized moxibustion at bilateral Xinshu (BL15) and Shenshu (BL23). Mice in the WT group and 5XFAD group received no treatment but were restrained to ensure exposure to a similar experimental condition. Cognitive function and memory were assessed with the Morris water maze and Y-maze tests. The amyloid β 40 (Aβ40) and amyloid β 42 (Aβ42) levels in the brain were evaluated by enzyme-linked immunosorbent assay; amyloid plaque deposition in brain tissue sections was detected by thioflavin-S staining; the expression of glial fibrillary acidic protein (GFAP), cluster of differentiation 11b (CD11b), brain-derived neurotrophic factor (BDNF), and choline acetyltransferase (ChAT) in the hippocampus and prefrontal cortex was analyzed by immunohistochemistry. Results: In the Morris water maze test, compared with the 5XFAD group, mice in the 5XFAD + GM group had a shorter escape latency and more target area crossings and spent more time in the target quadrant (P<0.05). In the Y-maze test, compared with the 5XFAD group, the number of training times of the 5XFAD + GM group was significantly decreased (P<0.05), together with more correct responses (P<0.05). Compared with the 5XFAD group, the levels of Aβ40 and Aβ42 in the brain tissue of the 5XFAD + GM group were significantly lower (P<0.05); in the hippocampus and prefrontal cortex, the total number of amyloid β plaque deposition were significantly lower (P<0.05); the expression levels of GFAP and CD11b were significantly reduced (P<0.05); and the expression levels of ChAT and BDNF were significantly increased (P<0.05).Conclusion: Grain-sized moxibustion at Xinshu (BL15) and Shenshu (BL23) greatly improves learning and memory functions, decreases the levels of Aβ40 and Aβ42, inhibits amyloid β plaque deposition, decreases the expression of GFAP and CD11b, and increases the expression of ChAT and BDNF in AD mice to inhibit the progression of AD. |
| Author | 龚晓炜 李书剑 张永生 樊振宇 楚佳梅 余静 包烨华 |
| Author_FL | YU Jing CHU Jiamei ZHANG Yongsheng FAN Zhenyu BAO Yehua GONG Xiaowei LI Shujian |
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| DocumentTitle_FL | Grain-sized moxibustion inhibits the progression of Alzheimer disease in 5XFAD transgenic mice |
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| Keywords | Amyloid β Deposit Moxibustion with Grain-sized Moxa Cone Astrocyte Alzheimer Disease β淀粉样沉积 Moxibustion Therapy Microglia 麦粒灸疗法 灸法 阿尔茨海默病 星形胶质细胞 小鼠 Mice 小胶质细胞 |
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| PublicationTitle_FL | Journal of Acupuncture and Tuina Science |
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| Title | 麦粒灸抑制5XFAD转基因模型小鼠阿尔茨海默病的发展 |
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