苦龙胆酯苷拮抗高脂饮食所致的小鼠动脉粥样硬化

R54%R966; [目的]探究苦龙胆酯苷(MAG)对高脂饮食(HFD)所致动脉粥样硬化的拮抗作用及其机制.[方法]将30只ApoE-/-小鼠随机分为对照组(正常饮食)、HFD组、HFD+MAG组,每组10只小鼠,其中HFD+MAG组每日予50 mg/kg MAG灌胃,持续12周.生物化学检测仪测定血脂水平;油红O染色观察主动脉斑块沉积情况;苏木精-伊红(HE)染色观察主动脉血管病理形态表现;Mac-3免疫组织化学染色观察主动脉巨噬细胞聚集情况;免疫荧光定位染色观察NOD样受体热蛋白结构域相关蛋白3(NLRP3)表达与分布;Western blot检测主动脉中NLRP3、凋亡相关斑点样蛋白(A...

Full description

Saved in:
Bibliographic Details
Published in中国动脉硬化杂志 Vol. 31; no. 5; pp. 399 - 403
Main Authors 张丽阳, 孙军, 陈迪, 张在行
Format Journal Article
LanguageChinese
Published 南阳市中心医院神经内科,河南省南阳市473000 2023
Subjects
NLRP3炎症小体
苦龙胆酯苷
高脂饮食
动脉粥样硬化
Online AccessGet full text
ISSN1007-3949
DOI10.3969/j.issn.1007-3949.2023.05.006

Cover

Abstract R54%R966; [目的]探究苦龙胆酯苷(MAG)对高脂饮食(HFD)所致动脉粥样硬化的拮抗作用及其机制.[方法]将30只ApoE-/-小鼠随机分为对照组(正常饮食)、HFD组、HFD+MAG组,每组10只小鼠,其中HFD+MAG组每日予50 mg/kg MAG灌胃,持续12周.生物化学检测仪测定血脂水平;油红O染色观察主动脉斑块沉积情况;苏木精-伊红(HE)染色观察主动脉血管病理形态表现;Mac-3免疫组织化学染色观察主动脉巨噬细胞聚集情况;免疫荧光定位染色观察NOD样受体热蛋白结构域相关蛋白3(NLRP3)表达与分布;Western blot检测主动脉中NLRP3、凋亡相关斑点样蛋白(ASC)和Caspase-1亚基p20表达水平.[结果]HFD+MAG组血清甘油三酯、总胆固醇和低密度脂蛋白胆固醇水平分别降低至HFD组的88.39％、74.85％和64.97％(均P＜0.05),高密度脂蛋白胆固醇水平升高至HFD组的142.18％(P＜0.05),斑块面积减少至HFD组的45.16％(P＜0.05),MAG能改善主动脉病理形态表现,使巨噬细胞沉积减少,斑块内NLRP3阳性巨噬细胞比例降低,主动脉中NLRP3、ASC和Caspase-1亚基p20表达分别降低至HFD组的45.58％、53.28％和42.35％(均P＜0.05).[结论]MAG对高脂饮食所致ApoE-/-小鼠动脉粥样硬化有明显的拮抗作用,其机制可能与其抑制斑块内巨噬细胞NLRP3炎症小体的激活有关.
AbstractList R54%R966; [目的]探究苦龙胆酯苷(MAG)对高脂饮食(HFD)所致动脉粥样硬化的拮抗作用及其机制.[方法]将30只ApoE-/-小鼠随机分为对照组(正常饮食)、HFD组、HFD+MAG组,每组10只小鼠,其中HFD+MAG组每日予50 mg/kg MAG灌胃,持续12周.生物化学检测仪测定血脂水平;油红O染色观察主动脉斑块沉积情况;苏木精-伊红(HE)染色观察主动脉血管病理形态表现;Mac-3免疫组织化学染色观察主动脉巨噬细胞聚集情况;免疫荧光定位染色观察NOD样受体热蛋白结构域相关蛋白3(NLRP3)表达与分布;Western blot检测主动脉中NLRP3、凋亡相关斑点样蛋白(ASC)和Caspase-1亚基p20表达水平.[结果]HFD+MAG组血清甘油三酯、总胆固醇和低密度脂蛋白胆固醇水平分别降低至HFD组的88.39％、74.85％和64.97％(均P＜0.05),高密度脂蛋白胆固醇水平升高至HFD组的142.18％(P＜0.05),斑块面积减少至HFD组的45.16％(P＜0.05),MAG能改善主动脉病理形态表现,使巨噬细胞沉积减少,斑块内NLRP3阳性巨噬细胞比例降低,主动脉中NLRP3、ASC和Caspase-1亚基p20表达分别降低至HFD组的45.58％、53.28％和42.35％(均P＜0.05).[结论]MAG对高脂饮食所致ApoE-/-小鼠动脉粥样硬化有明显的拮抗作用,其机制可能与其抑制斑块内巨噬细胞NLRP3炎症小体的激活有关.
Author 陈迪
张在行
张丽阳
孙军
AuthorAffiliation 南阳市中心医院神经内科,河南省南阳市473000
AuthorAffiliation_xml – name: 南阳市中心医院神经内科,河南省南阳市473000
Author_FL ZHANG Liyang
SUN Jun
ZHANG Zaihang
CHEN Di
Author_FL_xml – sequence: 1
  fullname: ZHANG Liyang
– sequence: 2
  fullname: SUN Jun
– sequence: 3
  fullname: CHEN Di
– sequence: 4
  fullname: ZHANG Zaihang
Author_xml – sequence: 1
  fullname: 张丽阳
– sequence: 2
  fullname: 孙军
– sequence: 3
  fullname: 陈迪
– sequence: 4
  fullname: 张在行
BookMark eNrjYmDJy89LZWBQMTTQM7Y0s9TP0sssLs7TMzQwMNc1tjSx1DMyMDLWMzDVMzAwY2HghItzMPAWF2cmGRgYGhoYmxkYcDI4vehe9nLfzBfNbS9b17_o3v6se92zrukvV8940dL0cum6l4vnP-tseNG-5fmslqcb-l_uWfC0a8WLls7nm5Y-W7D9-cI1T3um8TCwpiXmFKfyQmluBk031xBnD93yxLy0xLz0-Kz80qI8oEx8VXpKbmVGVRXIdQamQLcZk6IWAHgiXx0
ClassificationCodes R54%R966
ContentType Journal Article
Copyright Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
Copyright_xml – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
DBID 2B.
4A8
92I
93N
PSX
TCJ
DOI 10.3969/j.issn.1007-3949.2023.05.006
DatabaseName Wanfang Data Journals - Hong Kong
WANFANG Data Centre
Wanfang Data Journals
万方数据期刊 - 香港版
China Online Journals (COJ)
China Online Journals (COJ)
DatabaseTitleList
DeliveryMethod fulltext_linktorsrc
DocumentTitle_FL Amarogentin antagonizes atherosclerosis induced by high fat diet in mice
EndPage 403
ExternalDocumentID zgdmyhzz202305006
GrantInformation_xml – fundername: 河南省科技厅项目
  funderid: (192102310349)
GroupedDBID -05
2B.
4A8
5XA
5XF
92F
92I
93N
ABDBF
ABJNI
ACGFS
ALMA_UNASSIGNED_HOLDINGS
CCEZO
CIEJG
CW9
GROUPED_DOAJ
PSX
TCJ
TGQ
U1G
U5O
ID FETCH-wanfang_journals_zgdmyhzz2023050063
ISSN 1007-3949
IngestDate Thu May 29 04:06:36 EDT 2025
IsPeerReviewed true
IsScholarly true
Issue 5
Keywords NLRP3炎症小体
苦龙胆酯苷
高脂饮食
动脉粥样硬化
Language Chinese
LinkModel OpenURL
MergedId FETCHMERGED-wanfang_journals_zgdmyhzz2023050063
ParticipantIDs wanfang_journals_zgdmyhzz202305006
PublicationCentury 2000
PublicationDate 2023
PublicationDateYYYYMMDD 2023-01-01
PublicationDate_xml – year: 2023
  text: 2023
PublicationDecade 2020
PublicationTitle 中国动脉硬化杂志
PublicationTitle_FL Chinese Journal of Arteriosclerosis
PublicationYear 2023
Publisher 南阳市中心医院神经内科,河南省南阳市473000
Publisher_xml – name: 南阳市中心医院神经内科,河南省南阳市473000
SSID ssib001103600
ssib002262930
ssib051368528
ssj0000602818
Score 4.6666965
Snippet R54%R966; [目的]探究苦龙胆酯苷(MAG)对高脂饮食(HFD)所致动脉粥样硬化的拮抗作用及其机制.[方法]将30只ApoE-/-小鼠随机分为对照组(正常饮食)、HFD组、HFD+MAG组,每组10只小...
SourceID wanfang
SourceType Aggregation Database
StartPage 399
Title 苦龙胆酯苷拮抗高脂饮食所致的小鼠动脉粥样硬化
URI https://d.wanfangdata.com.cn/periodical/zgdmyhzz202305006
Volume 31
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
journalDatabaseRights – providerCode: PRVAON
  databaseName: DOAJ Directory of Open Access Journals
  issn: 1007-3949
  databaseCode: DOA
  dateStart: 20210101
  customDbUrl:
  isFulltext: true
  dateEnd: 99991231
  titleUrlDefault: https://www.doaj.org/
  omitProxy: true
  ssIdentifier: ssj0000602818
  providerName: Directory of Open Access Journals
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LaxRBEB5iBPEiiopvglgHD7vOe7qP3bOzBA-eIuQWdmZ3Ew-uEDcH9yS6KskK3nxdjEhEUKKIkIuIf2Zf_8Kqms5mNCFGL0NTU3z1ddXuVHXTD8u64qReM4iajVLYcKOS76R-SXhhVpJp5opGZKdZxqt8b4SzN_3r88H81KGfhVVLK-20nHX23FfyP1FFGcaVdsn-Q2QnoCjANsYXnxhhfB4oxpAIEBpUCIkEnYCULPFAsEQEoKpGR0eQhKyccEOBjEhHaZCCdbCodFkSsA42PJBVVpa0HoJ0ItA-JBFIRfpJANoGUWXrMSibJIisJoCSlLVLmIiDCkQjAuWAilk5hvym2e36GBIftABVobcSaVf2xvwdgcBlhfkjpSrISXJlQU6NgQlPUo-1V1RBe-S7gBwn9c4bSWLBphFWqT1gkWZs2CEpERenUfI9zvyTZ_CKcTrbZwzBpAt9RjPCMx3T2jBQLvcZTSXsUM1OZ7YYY3qFIcGUFJMj0N05uDEXEUHh7EPAp9sE7EJ6oollT-aHvG7nL5NFbxVXCHAy8vKrp0xd4_NZErtSpidDySmTDJQnBsrkIz7T1v7jpHKufTqL9dv3ljod0rIDPvP-sBthrVeY1OCC3MEKyS7utA5duTNgDRy6_8AMgPPayabjyXiJgmFyBD8oOc1r-5HkTXetZq21WKgP545bx8zAbkbl_9IT1lRn6aSlR7334x-vRg8fjx99HvW2Br3NwdqL8ceXo-6D8cbm-N2bwer90ZNvw9fd_pdn4-_r_bUPo-7q8OvGYH1r-PZT_-nzU9bVajIXz5aM3QXzZbi7sMs73mlrunWn1ThjzYRh5gZpLZAuDrxqjUxg1mymaVBPw1TUm82z1uW_4507iNJ56yi18_nCC9Z0e3mlcREr6HZ6iYP0C7Mklvg
linkProvider Directory of Open Access Journals
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E8%8B%A6%E9%BE%99%E8%83%86%E9%85%AF%E8%8B%B7%E6%8B%AE%E6%8A%97%E9%AB%98%E8%84%82%E9%A5%AE%E9%A3%9F%E6%89%80%E8%87%B4%E7%9A%84%E5%B0%8F%E9%BC%A0%E5%8A%A8%E8%84%89%E7%B2%A5%E6%A0%B7%E7%A1%AC%E5%8C%96&rft.jtitle=%E4%B8%AD%E5%9B%BD%E5%8A%A8%E8%84%89%E7%A1%AC%E5%8C%96%E6%9D%82%E5%BF%97&rft.au=%E5%BC%A0%E4%B8%BD%E9%98%B3&rft.au=%E5%AD%99%E5%86%9B&rft.au=%E9%99%88%E8%BF%AA&rft.au=%E5%BC%A0%E5%9C%A8%E8%A1%8C&rft.date=2023&rft.pub=%E5%8D%97%E9%98%B3%E5%B8%82%E4%B8%AD%E5%BF%83%E5%8C%BB%E9%99%A2%E7%A5%9E%E7%BB%8F%E5%86%85%E7%A7%91%2C%E6%B2%B3%E5%8D%97%E7%9C%81%E5%8D%97%E9%98%B3%E5%B8%82473000&rft.issn=1007-3949&rft.volume=31&rft.issue=5&rft.spage=399&rft.epage=403&rft_id=info:doi/10.3969%2Fj.issn.1007-3949.2023.05.006&rft.externalDocID=zgdmyhzz202305006
thumbnail_s http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fzgdmyhzz%2Fzgdmyhzz.jpg