PURIFYING AND POLYMERIZING 3'-BLOCKED NUCLEOTIDES

• Main Authors: IMSAND, Erin, MCCAULEY, Patrick, MILLER, Oliver, LAWRENCE, Elliot, MOSCHETTI, Tommaso, JACKSON, Amanda, PANCHAPAKESAN, Rajagopal, CHESNEY, Michael, RICHARDSON, Marta, JACKSON, Rosamond, SMITH, Dominic, DAY, Henry, MASON, Stephen, DREWS, Bradley, SCHNEIDER, Kim, BETLEY, Jason
• Format: Patent
• Language: English; French
• Published: 01.12.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; VINEGAR; WINE

A method for purifying nucleotides is provided, that includes preparing a solution comprising (a) 3'-blocked nucleotides, (b) 3'-OH nucleotides, (c) a polishing polymerase, and (d) a template. The polishing polymerase and the template are used to selectively polymerize the 3'-OH nucleotides and thus reduce a concentration in the solution of the 3 '-OH nucleotides relative to the 3'-blocked nucleotides. L'invention concerne un procédé de purification de nucléotides qui comprend la préparation d'une solution comprenant (a) des nucléotides bloqués en 3', (b) des nucléotides 3'-OH, (c) une polymérase de polissage et (d) un modèle. La polymérase de polissage et le modèle sont utilisés pour polymériser sélectivement les nucléotides 3'-OH et réduire ainsi une concentration dans la solution des nucléotides 3'-OH par rapport aux nucléotides bloqués en 3'.