基于波前校正的双光子全息光遗传学刺激方法、系统、设备及介质

• Format: Patent
• Language: Chinese
• Published: 03.10.2025
• Subjects: CALCULATING; COMPUTING; COUNTING; HEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATIONTECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING ORPROCESSING OF MEDICAL OR HEALTHCARE DATA; IMAGE DATA PROCESSING OR GENERATION, IN GENERAL; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; PHYSICS

本发明涉及一种基于波前校正的双光子全息光遗传学刺激方法、系统、设备及介质，包括：步骤S1、从双光子成像中选取目标神经元，获取目标神经元的三维空间位置对应的全息图；步骤S2、在全息图上为目标神经元选择需要进行波前畸变校正的目标区域，并在目标区域对目标神经元进行波前畸变校正，得到包含波前畸变信息和位置信息的相位图；步骤S3、基于包含波前畸变信息和位置信息的相位图，对目标神经元进行光激发。与现有技术相比，本发明实现大视场更低激发功率更多神经元的深层单细胞分辨率激发，显著提升光遗传学刺激成像的精确性和安全性。 The invention relates to a wavefront correction-based two-photon holographic optogenetics stimulation method, system and device and a medium, and the method comprises the steps: S1, selecting a target neuron from two-photon imaging, and obtaining a hologram corresponding to a three-dimensional space position of the target neuron; s2, selecting a target area needing wavefront distortion correction for the target neuron on the hologram, and performing wavefront distortion correction on the target neuron in the target area to obtain a phase diagram containing wavefront distortion information and position information; and S3, performing optical excitation on the target neurons based on the phase diagram containing the wavefront distortion information and the posit