Essential Amino Acids Increase MicroRNA-499, −208b, and −23a and Downregulate Myostatin and Myocyte Enhancer Factor 2C mRNA Expression in Human Skeletal Muscle12
Essential amino acids (EAA) stimulate muscle protein synthesis in humans. However, little is known about whether microRNAs (miRNA) and genes associated with muscle growth are expressed differently following EAA ingestion. Our purpose in this experiment was to determine whether miRNA and growth-relat...
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| Published in | The Journal of nutrition Vol. 139; no. 12; pp. 2279 - 2284 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Elsevier Inc
01.12.2009
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0022-3166 1541-6100 |
| DOI | 10.3945/jn.109.112797 |
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| Abstract | Essential amino acids (EAA) stimulate muscle protein synthesis in humans. However, little is known about whether microRNAs (miRNA) and genes associated with muscle growth are expressed differently following EAA ingestion. Our purpose in this experiment was to determine whether miRNA and growth-related mRNA expressed in skeletal muscle are up- or downregulated in humans following the ingestion of EAA. We hypothesized that EAA would alter miRNA expression in skeletal muscle as well as select growth-related genes. Muscle biopsies were obtained from the vastus lateralis of 7 young adult participants (3 male, 4 female) before and 3 h after ingesting 10 g of EAA. Muscle samples were analyzed for muscle miRNA (miR-499, −208b, −23a, −1, −133a, and −206) and muscle-growth related genes [MyoD1, myogenin, myostatin, myocyte enhancer factor C (MEF2C), follistatin-like-1 (FSTL1), histone deacytylase 4, and serum response factor mRNA] before and after EAA ingestion using real-time PCR. Following EAA ingestion, miR-499, −208b, −23a, −1, and pri-miR-206 expression increased (P < 0.05). The muscle-growth genes MyoD1 and FSTL1 mRNA expression increased (P < 0.05), and myostatin and MEF2C mRNA were downregulated following EAA ingestion (P < 0.05). We conclude that miRNA and growth-related genes expressed in skeletal muscle are rapidly altered within hours following EAA ingestion. Further work is needed to determine whether these miRNA are post-transcriptional regulators of growth-related genes following an anabolic stimulus. |
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| AbstractList | Essential amino acids (EAA) stimulate muscle protein synthesis in humans. However, little is known about whether microRNAs (miRNA) and genes associated with muscle growth are expressed differently following EAA ingestion. Our purpose in this experiment was to determine whether miRNA and growth-related mRNA expressed in skeletal muscle are up- or downregulated in humans following the ingestion of EAA. We hypothesized that EAA would alter miRNA expression in skeletal muscle as well as select growth-related genes. Muscle biopsies were obtained from the vastus lateralis of 7 young adult participants (3 male, 4 female) before and 3 h after ingesting 10 g of EAA. Muscle samples were analyzed for muscle miRNA (miR-499, −208b, −23a, −1, −133a, and −206) and muscle-growth related genes [MyoD1, myogenin, myostatin, myocyte enhancer factor C (MEF2C), follistatin-like-1 (FSTL1), histone deacytylase 4, and serum response factor mRNA] before and after EAA ingestion using real-time PCR. Following EAA ingestion, miR-499, −208b, −23a, −1, and pri-miR-206 expression increased (P < 0.05). The muscle-growth genes MyoD1 and FSTL1 mRNA expression increased (P < 0.05), and myostatin and MEF2C mRNA were downregulated following EAA ingestion (P < 0.05). We conclude that miRNA and growth-related genes expressed in skeletal muscle are rapidly altered within hours following EAA ingestion. Further work is needed to determine whether these miRNA are post-transcriptional regulators of growth-related genes following an anabolic stimulus. |
| Author | Glynn, Erin L. Drummond, Micah J. Dhanani, Shaheen Fry, Christopher S. Volpi, Elena Rasmussen, Blake B. |
| Author_xml | – sequence: 1 givenname: Micah J. surname: Drummond fullname: Drummond, Micah J. email: mjdrummo@utmb.edu organization: Department of Physical Therapy, University of Texas Medical Branch, Galveston, TX 77555 – sequence: 2 givenname: Erin L. surname: Glynn fullname: Glynn, Erin L. organization: Division of Rehabilitation Science, University of Texas Medical Branch, Galveston, TX 77555 – sequence: 3 givenname: Christopher S. surname: Fry fullname: Fry, Christopher S. organization: Division of Rehabilitation Science, University of Texas Medical Branch, Galveston, TX 77555 – sequence: 4 givenname: Shaheen surname: Dhanani fullname: Dhanani, Shaheen organization: Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX 77555 – sequence: 5 givenname: Elena surname: Volpi fullname: Volpi, Elena organization: Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX 77555 – sequence: 6 givenname: Blake B. surname: Rasmussen fullname: Rasmussen, Blake B. organization: Department of Physical Therapy, University of Texas Medical Branch, Galveston, TX 77555 |
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| Keywords | HDAC4 MEF2C EAA miR-23a SRF miR-1 miR-133a pri-miR-1-2 FSTL1 miR-206 pri-miR-206 miR-499 miR-208b pri-miR-133a-1 miRNA |
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| Title | Essential Amino Acids Increase MicroRNA-499, −208b, and −23a and Downregulate Myostatin and Myocyte Enhancer Factor 2C mRNA Expression in Human Skeletal Muscle12 |
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