Improved visualization of oral microbial consortia- Associated images

• Main Authors: Ramirez Puebla, Shamayim Tabita, Mark Welch, Jessica, Borisy, Gary
• Format: Image
• Language: English
• Published: Zenodo 22.04.2024
• Subjects: Biofilms; Imaging; Microbial ecology; Oral microbiome; Tongue biofilm
• ISSN: 0022-0345
• DOI: 10.5281/zenodo.11042810

These images are associated to the paper "Improved visualization of oral microbial consortia" published in the Journal of Dental Research. Sample author: Tabita Ramirez Puebla Images show microbial consortia from human tongue dorsum biofilm. Imaged in a confocal microscope (Zeiss LSM 780) with a spectral detector (32 channels). Objective: Plan-Apochromat 63X; N.A. 1.4; Oil  Pixel size: 0.07 um x 0.07 um Image size (pixels): 2048 x 2048 Optical section : 1 micron   File descriptions TIFF files in Image5D format. Files resulted from linear unmixing performed with Zeiss ZEN algorithm (ZEN Black) or using the non-linear least-squares function in MATLAB. Individual fluorophore and autofluorescence channels are presented.  Fig2A_5DFig2C_5DFig4_zstack_5D (zstack with 12 optical slices) jpg files of pseudocolored imagesFig2A_jpegFig2B_jpegFig2C_jpegFig2D_jpegFig3A_jpeg_stack_RGB_tif (stack of 257 optical slices RGB images in tif format)Fig3A_Montage20x13_jpeg (Montage of 257 optical slices)Fig3B_jpegFig3C_jpegFig3D_jpegFig4A_xy (view of xy plane)Fig4A_xz_orthogonal (view of xy plane -> orthogonal representation of 12 optical slices)Fig4A_yz_orthogonal (view of yz plane -> orthogonal representation of 12 optical slices)Fig4B_jpegFig4C_jpeg Representative Zeiss .czi (raw files from LSM780 confocal microscope)Fig2A_raw (original czi file)Fig2C_raw (original czi file)