High-resolution analysis of bound Ca 2+ in neurons and synapses
Calcium (Ca 2+ ) is a well-known second messenger in all cells, and is especially relevant for neuronal activity. Neuronal Ca 2+ is found in different forms, with a minority being freely soluble in the cell and more than 99% being bound to proteins. Free Ca 2+ has received much attention over the la...
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| Published in | Life science alliance Vol. 7; no. 1; p. e202302030 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
01.01.2024
|
| Online Access | Get full text |
| ISSN | 2575-1077 2575-1077 |
| DOI | 10.26508/lsa.202302030 |
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| Summary: | Calcium (Ca
2+
) is a well-known second messenger in all cells, and is especially relevant for neuronal activity. Neuronal Ca
2+
is found in different forms, with a minority being freely soluble in the cell and more than 99% being bound to proteins. Free Ca
2+
has received much attention over the last few decades, but protein-bound Ca
2+
has been difficult to analyze. Here, we introduce correlative fluorescence and nanoscale secondary ion mass spectrometry imaging as a tool to describe bound Ca
2+
. As expected, bound Ca
2+
is ubiquitous. It does not correlate to free Ca
2+
dynamics at the whole-neuron level, but does correlate significantly to the intensity of markers for GABAergic pre-synapse and glutamatergic post-synapses. In contrast, a negative correlation to pre-synaptic activity was observed, with lower levels of bound Ca
2+
observed in the more active synapses. We conclude that bound Ca
2+
may regulate neuronal activity and should receive more attention in the future. |
|---|---|
| ISSN: | 2575-1077 2575-1077 |
| DOI: | 10.26508/lsa.202302030 |