基于表面等离子体共振的外泌体PD-L1蛋白检测技术
Q816; 血清中可溶性程序性死亡-配体1(programmed cell death 1 ligand 1,PD-L1)蛋白的存在形式包括外泌体表面、微囊泡表面以及分泌的游离形式等.前期研究表明,血清中外泌体表面PD-L1含量与多种癌症的预后评估显著相关,然而目前的常规检测方法通常将血清中所有可溶性PD-L1蛋白作为一个整体进行检测,无法有效分辨外泌体PD-L1与其他形式的PD-L1.本研究基于表面等离子体共振技术,建立了一种外泌体PD-L1的特异性检测方法.该方法首先通过抗体识别PD-L1蛋白,并将其捕获至检测芯片表面;再利用α-溶血素在外泌体膜上形成多个寡聚体,从而特异性检测外泌体PD-...
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| Published in | 中国生物化学与分子生物学报 Vol. 40; no. 9; pp. 1300 - 1307 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
浙江大学农业与生物技术学院现代种业研究所,杭州 310058
20.09.2024
中国计量大学生命科学学院,浙江省生物计量及检验检疫技术重点实验室,杭州 310018%中国计量大学生命科学学院,浙江省生物计量及检验检疫技术重点实验室,杭州 310018 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1007-7626 |
| DOI | 10.13865/j.cnki.cjbmb.2024.07.1136 |
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| Abstract | Q816; 血清中可溶性程序性死亡-配体1(programmed cell death 1 ligand 1,PD-L1)蛋白的存在形式包括外泌体表面、微囊泡表面以及分泌的游离形式等.前期研究表明,血清中外泌体表面PD-L1含量与多种癌症的预后评估显著相关,然而目前的常规检测方法通常将血清中所有可溶性PD-L1蛋白作为一个整体进行检测,无法有效分辨外泌体PD-L1与其他形式的PD-L1.本研究基于表面等离子体共振技术,建立了一种外泌体PD-L1的特异性检测方法.该方法首先通过抗体识别PD-L1蛋白,并将其捕获至检测芯片表面;再利用α-溶血素在外泌体膜上形成多个寡聚体,从而特异性检测外泌体PD-L1.该方法通过α-溶血素结合过程的信号变化对外泌体PD-L1含量进行检测,可以有效降低检测背景噪音并放大信号.使用α-溶血素放大信号前的线性范围为0.035～2.208 pg/mL,信号放大后的线性范围为0.004-0.552 pg/mL.方法学验证实验表明,该方法特异性、灵敏度和精密度良好,具有一定的临床应用前景. |
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| AbstractList | Q816; 血清中可溶性程序性死亡-配体1(programmed cell death 1 ligand 1,PD-L1)蛋白的存在形式包括外泌体表面、微囊泡表面以及分泌的游离形式等.前期研究表明,血清中外泌体表面PD-L1含量与多种癌症的预后评估显著相关,然而目前的常规检测方法通常将血清中所有可溶性PD-L1蛋白作为一个整体进行检测,无法有效分辨外泌体PD-L1与其他形式的PD-L1.本研究基于表面等离子体共振技术,建立了一种外泌体PD-L1的特异性检测方法.该方法首先通过抗体识别PD-L1蛋白,并将其捕获至检测芯片表面;再利用α-溶血素在外泌体膜上形成多个寡聚体,从而特异性检测外泌体PD-L1.该方法通过α-溶血素结合过程的信号变化对外泌体PD-L1含量进行检测,可以有效降低检测背景噪音并放大信号.使用α-溶血素放大信号前的线性范围为0.035～2.208 pg/mL,信号放大后的线性范围为0.004-0.552 pg/mL.方法学验证实验表明,该方法特异性、灵敏度和精密度良好,具有一定的临床应用前景. |
| Abstract_FL | Soluble programmed cell death 1 ligand 1(PD-L1)in the serum includes exosomal,mi-crovesical and secreted forms of PD-L1.Previous studies have shown that the level of exosomal PD-L1 in the serum significantly correlated with the prognosis of various cancers.However,current analysis detects all forms of PD-L1 in the serum as a whole,without distinguishing exosomal PD-L1 from other forms.In this study,a specific detection method for exosomal PD-L1 was established based on surface plasmon res-onance.This method first captures PD-L1 by antibody recognition and immobilizes it on the surface of the detection chip.Then,α-hemolysin was recruited to form multiple oligomers on the exosomal membrane.This method quantifies the content of exosomal PD-L1 by monitoring the signal change during the binding process of α-hemolysin,effectively reducing background noises and amplifying the signal.The linear range before signal amplification with α-hemolysin was 0.035-2.208 pg/mL,and after signal amplifica-tion,it was 0.004-0.552 pg/mL.Methodological validation showed that this method has good specifici-ty,sensitivity,and repeatability,and has certain clinical application prospects. |
| Author | 叶子弘 夏文强 屈丽思 俞泽涛 彭宇彦 |
| AuthorAffiliation | 中国计量大学生命科学学院,浙江省生物计量及检验检疫技术重点实验室,杭州 310018%中国计量大学生命科学学院,浙江省生物计量及检验检疫技术重点实验室,杭州 310018;浙江大学农业与生物技术学院现代种业研究所,杭州 310058 |
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| Author_FL | YE Zi-Hong QU Li-Si PENG Yu-Yan YU Ze-Tao XIA Wen-Qiang |
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| DocumentTitle_FL | Exosomal PD-L1 Detection Methods Based on Surface Plasmon Resonance |
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| Keywords | α-hemolysin programmed cell death 1 ligand 1(PD-L1) α-溶血素 外泌体 生物标志物 biomarker exosome 程序性死亡-配体1 |
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| Title | 基于表面等离子体共振的外泌体PD-L1蛋白检测技术 |
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