CpG-ODN对重离子辐射所致脾脏损伤的救治作用

R3; 本次研究主要探讨CpG-ODN对重离子辐射所致脾脏损伤的救治作用.实验中C57BL/6L小鼠受到5 Gy 12C6+离子全身照射,在照射后急性期计算小鼠脾脏指数,苏木精-伊红(Hematoxylin-Eosin staining,HE)染色观察脾脏组织结构变化.另外,免疫组化法检测γ-H2AX蛋白含量以判断脾脏细胞DNA发生双链断裂((Double-strand break,DSB)情况,末端脱氧核苷酸转移酶介导的dUTP缺口末端标(Terminal deoxynucleotidyl transferase-mediated dUTP nick and labeling,TUNEL)法...

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Published in辐射研究与辐射工艺学报 Vol. 41; no. 4; pp. 43 - 50
Main Authors 张超, 傅华, 易永华, 蔡建明, 高福
Format Journal Article
LanguageChinese
Published 中国人民解放军联勤保障部队第九四〇医院 放疗科 兰州 730050%中国人民解放军海军军医大学 放射医学教研室 上海 200433 01.08.2023
Subjects
Spleen
Double-strand break(DSB)
碳离子辐射
脾脏
Carbon ion radiation(CIR)
双链断裂
CpG-ODN
凋亡
Apoptosis
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ISSN1000-3436
DOI10.11889/j.1000-3436.2022-0116

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Abstract R3; 本次研究主要探讨CpG-ODN对重离子辐射所致脾脏损伤的救治作用.实验中C57BL/6L小鼠受到5 Gy 12C6+离子全身照射,在照射后急性期计算小鼠脾脏指数,苏木精-伊红(Hematoxylin-Eosin staining,HE)染色观察脾脏组织结构变化.另外,免疫组化法检测γ-H2AX蛋白含量以判断脾脏细胞DNA发生双链断裂((Double-strand break,DSB)情况,末端脱氧核苷酸转移酶介导的dUTP缺口末端标(Terminal deoxynucleotidyl transferase-mediated dUTP nick and labeling,TUNEL)法检测脾脏细胞凋亡水平,荧光标记法检测外周血CD19+B细胞数量.结果显示,经12C6+离子照射后CpG-ODN处理,小鼠脾脏指数提高,脾脏红髓萎缩程度减轻,白髓面积增大,其内淋巴B细胞排列密度提高,发生DSB和凋亡的细胞数量减少,外周血CD19+B细胞数量增加.这些表明CpG-ODN能减轻12C6+离子照射后脾脏损伤,其原因可能与CpG-ODN抑制细胞发生DSB和凋亡有关.
AbstractList R3; 本次研究主要探讨CpG-ODN对重离子辐射所致脾脏损伤的救治作用.实验中C57BL/6L小鼠受到5 Gy 12C6+离子全身照射,在照射后急性期计算小鼠脾脏指数,苏木精-伊红(Hematoxylin-Eosin staining,HE)染色观察脾脏组织结构变化.另外,免疫组化法检测γ-H2AX蛋白含量以判断脾脏细胞DNA发生双链断裂((Double-strand break,DSB)情况,末端脱氧核苷酸转移酶介导的dUTP缺口末端标(Terminal deoxynucleotidyl transferase-mediated dUTP nick and labeling,TUNEL)法检测脾脏细胞凋亡水平,荧光标记法检测外周血CD19+B细胞数量.结果显示,经12C6+离子照射后CpG-ODN处理,小鼠脾脏指数提高,脾脏红髓萎缩程度减轻,白髓面积增大,其内淋巴B细胞排列密度提高,发生DSB和凋亡的细胞数量减少,外周血CD19+B细胞数量增加.这些表明CpG-ODN能减轻12C6+离子照射后脾脏损伤,其原因可能与CpG-ODN抑制细胞发生DSB和凋亡有关.
Author 张超
蔡建明
傅华
高福
易永华
AuthorAffiliation 中国人民解放军联勤保障部队第九四〇医院 放疗科 兰州 730050%中国人民解放军海军军医大学 放射医学教研室 上海 200433
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Author_FL YI Yonghua
ZHANG Chao
GAO Fu
FU Hua
CAI Jianming
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Keywords Spleen
Double-strand break(DSB)
碳离子辐射
脾脏
Carbon ion radiation(CIR)
双链断裂
CpG-ODN
凋亡
Apoptosis
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Title CpG-ODN对重离子辐射所致脾脏损伤的救治作用
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