小鼠血清中对电离辐射敏感的蛋白分子研究
Q691; 拟寻找血清中对电离辐射敏感的蛋白分子,探讨其作为辐射生物标志物的潜力.利用蛋白抗体芯片检测经不同剂量X射线照射后24 h小鼠血清的蛋白分子表达谱,发现47种表达差异超过1.5倍的蛋白,其中胰岛素样生长因子1和2 (IGF-1和IGF-2)、胰岛素样生长因子结合蛋白1和3(IGFBP-1和IGFBP-3)丰度较高,与辐射损伤和修复相关且同属于IGFs-IGFBPs调控通路,利用酶联免疫吸附测定技术进一步研究这4种分子和其上游调控蛋白——生长因子(GH)的剂量效应和时间效应.结果显示,与对照组(0 Gy)相比:GH和IGF-1血清水平在照射(0.5、2、4 Gy)后24 h变化趋势不明...
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| Published in | 辐射研究与辐射工艺学报 Vol. 37; no. 6; pp. 18 - 25 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
甘肃省空间辐射生物学重点实验室 中国科学院近代物理研究所 兰州 730000
01.12.2019
中国科学院大学 北京 100049%甘肃省空间辐射生物学重点实验室 中国科学院近代物理研究所 兰州 730000 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1000-3436 |
| DOI | 10.11889/j.1000-3436.2019.rrj.37.060301 |
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| Abstract | Q691; 拟寻找血清中对电离辐射敏感的蛋白分子,探讨其作为辐射生物标志物的潜力.利用蛋白抗体芯片检测经不同剂量X射线照射后24 h小鼠血清的蛋白分子表达谱,发现47种表达差异超过1.5倍的蛋白,其中胰岛素样生长因子1和2 (IGF-1和IGF-2)、胰岛素样生长因子结合蛋白1和3(IGFBP-1和IGFBP-3)丰度较高,与辐射损伤和修复相关且同属于IGFs-IGFBPs调控通路,利用酶联免疫吸附测定技术进一步研究这4种分子和其上游调控蛋白——生长因子(GH)的剂量效应和时间效应.结果显示,与对照组(0 Gy)相比:GH和IGF-1血清水平在照射(0.5、2、4 Gy)后24 h变化趋势不明显,IGF-2、IGFBP-1和IGFBP-3表达水平都具有上升趋势,特别是IGFBP-3,在照射(0.5、2、4 Gy)后表达水平都显著升高(p<0.01),且有明显的剂量依赖效应;在不同剂量X射线照射后的2~24 h内,GH表达水平波动较大,IGF-1无显著变化, IGF-2、IGFBP-1和IGFBP-3表达水平随时间延长有上升趋势,其中,IGFBP-1和IGFBP-3在照射后2~24 h都有较为稳定的表达上调趋势.以上结果表明,血清IGF-2、IGFBP-1和IGFBP-3在X射线照射后表达水平升高,有较好的剂量依赖效应和随时间变化的稳定性,具有作为电离辐射标志物的巨大潜力. |
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| AbstractList | Q691; 拟寻找血清中对电离辐射敏感的蛋白分子,探讨其作为辐射生物标志物的潜力.利用蛋白抗体芯片检测经不同剂量X射线照射后24 h小鼠血清的蛋白分子表达谱,发现47种表达差异超过1.5倍的蛋白,其中胰岛素样生长因子1和2 (IGF-1和IGF-2)、胰岛素样生长因子结合蛋白1和3(IGFBP-1和IGFBP-3)丰度较高,与辐射损伤和修复相关且同属于IGFs-IGFBPs调控通路,利用酶联免疫吸附测定技术进一步研究这4种分子和其上游调控蛋白——生长因子(GH)的剂量效应和时间效应.结果显示,与对照组(0 Gy)相比:GH和IGF-1血清水平在照射(0.5、2、4 Gy)后24 h变化趋势不明显,IGF-2、IGFBP-1和IGFBP-3表达水平都具有上升趋势,特别是IGFBP-3,在照射(0.5、2、4 Gy)后表达水平都显著升高(p<0.01),且有明显的剂量依赖效应;在不同剂量X射线照射后的2~24 h内,GH表达水平波动较大,IGF-1无显著变化, IGF-2、IGFBP-1和IGFBP-3表达水平随时间延长有上升趋势,其中,IGFBP-1和IGFBP-3在照射后2~24 h都有较为稳定的表达上调趋势.以上结果表明,血清IGF-2、IGFBP-1和IGFBP-3在X射线照射后表达水平升高,有较好的剂量依赖效应和随时间变化的稳定性,具有作为电离辐射标志物的巨大潜力. |
| Abstract_FL | ABSTRACT This study aimed to identify serum protein responses to ionizing radiation and to indicate their capacity for serving as radiation biomarkers. We performed antibody-based protein microarray studies to determine the expression profiles of serum proteins in mice 24 h after varying doses of X-ray irradiation. A set of 47 serum proteins with relative increases in expression of≥1.5 fold was identified. Among them, insulin-like growth factors 1 and 2 (IGF-1 and IGF-2), and insulin-like growth factor binding proteins 1 and 3 (IGFBP-1 and IGFBP-3) had higher abundance and were reported to be involved in radiation-induced DNA damage and repair through the IGF-IGFBP axis. Therefore, these four proteins and their upstream regulatory protein-growth factor (GH) were selected for further validation and assessment of their dose and time dependency by enzyme-linked immune sorbent assay. The results revealed that levels of serum GH and IGF-1 were not significantly different from those in the control group (0 Gy) after 0.5, 2, and 4 Gy of X-ray irradiation, while levels of serum IGF-2, IGFBP-1, and IGFBP-3 showed an upward trend. Levels of IGFBP-3 were significantly increased, with an obvious dose-dependence (p<0.01). Moreover, between 2 and 24 h post-irradiation, expression of serum GH fluctuated greatly, while IGF-1 showed no significant changes, and levels of IGF-2, IGFBP-1, and IGFBP-3 increased. The upward trends in IGFBP-1 and IGFBP-3 expression were evident from 2 to 24 h, at least. These results demonstrated that levels of serum IGF-2, IGFBP-1, and IGFBP-3 showed significant increases with good dose and time dependent effects after X-ray irradiation, suggesting that these proteins have great potential as biomarkers of ionizing radiation. |
| Author | 马伟 白皓 危文俊 王菊芳 |
| AuthorAffiliation | 甘肃省空间辐射生物学重点实验室 中国科学院近代物理研究所 兰州 730000;中国科学院大学 北京 100049%甘肃省空间辐射生物学重点实验室 中国科学院近代物理研究所 兰州 730000 |
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| Author_FL | BAI Hao WEI Wenjun WANG Jufang MA Wei |
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| DocumentTitle_FL | Study on radio-sensitive proteins to ionizing radiation in mice serum |
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| Keywords | Ionizing radiation 血清 结合蛋白 电离辐射 生物标志物 Biomarkers Serum Bindin 胰岛素样生长因子 Insulin-like growth factors |
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| PublicationTitle | 辐射研究与辐射工艺学报 |
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| Publisher | 甘肃省空间辐射生物学重点实验室 中国科学院近代物理研究所 兰州 730000 中国科学院大学 北京 100049%甘肃省空间辐射生物学重点实验室 中国科学院近代物理研究所 兰州 730000 |
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