基于HPLC指纹图谱结合化学模式识别以及多指标成分定量评价前胡药材质量
R284.1; 基于HPLC指纹图谱和多指标成分含量测定,并结合化学模式识别,评价不同产地前胡药材质量.采用Agi-lent SB-C18(4.6 mm × 250 mm,5 μm)色谱柱,以甲醇-0.5％甲酸水为流动相进行梯度洗脱,流速为0.5 mL/min,检测波长321 nm,建立指纹图谱并对4个香豆素类成分进行定量,采用聚类分析(hierarchical clustering analysis,HCA)、主成分分析(principal component analysis,PCA)、偏最小二乘法-判别分析(partial least squares discriminant analy...
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| Published in | 天然产物研究与开发 Vol. 36; no. 3; pp. 433 - 443 |
|---|---|
| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
湖南中医药大学药学院,长沙 410208%湖南省中医药研究院中药资源研究所,长沙 410013
20.03.2024
湖南省中医药研究院中药资源研究所,长沙 410013 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-6880 |
| DOI | 10.16333/j.1001-6880.2024.3.007 |
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| Abstract | R284.1; 基于HPLC指纹图谱和多指标成分含量测定,并结合化学模式识别,评价不同产地前胡药材质量.采用Agi-lent SB-C18(4.6 mm × 250 mm,5 μm)色谱柱,以甲醇-0.5％甲酸水为流动相进行梯度洗脱,流速为0.5 mL/min,检测波长321 nm,建立指纹图谱并对4个香豆素类成分进行定量,采用聚类分析(hierarchical clustering analysis,HCA)、主成分分析(principal component analysis,PCA)、偏最小二乘法-判别分析(partial least squares discriminant analysis,PLS-DA)对不同产地前胡药材进行质量评价.结果显示16批前胡药材HPLC指纹图谱相似度为0.966～0.999,确定了 21个共有峰,共指认了 9个成分.通过化学模式识别分析,将16批样品聚类为三类:以安徽宁国、亳州等为主的道地产区前胡,以湖南、贵州、重庆等为主的前胡,以及高海拔云南产区的前胡,并确立8个差异性质量标志物.多指标含量测定不同产地前胡的佛手柑内酯、白花前胡甲素、白花前胡乙素、白花前胡素E含量分别为0.016 7～0.131 9 mg/g、6.0 696～16.225 5 mg/g、0.426 5～2.010 0 mg/g、1.340 7～3.115 5 mg/g.本研究所建立的指纹图谱和含量测定方法专属性强,稳定性高,分离度良好,结合化学模式可用于前胡药材的质量评价. |
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| AbstractList | R284.1; 基于HPLC指纹图谱和多指标成分含量测定,并结合化学模式识别,评价不同产地前胡药材质量.采用Agi-lent SB-C18(4.6 mm × 250 mm,5 μm)色谱柱,以甲醇-0.5％甲酸水为流动相进行梯度洗脱,流速为0.5 mL/min,检测波长321 nm,建立指纹图谱并对4个香豆素类成分进行定量,采用聚类分析(hierarchical clustering analysis,HCA)、主成分分析(principal component analysis,PCA)、偏最小二乘法-判别分析(partial least squares discriminant analysis,PLS-DA)对不同产地前胡药材进行质量评价.结果显示16批前胡药材HPLC指纹图谱相似度为0.966～0.999,确定了 21个共有峰,共指认了 9个成分.通过化学模式识别分析,将16批样品聚类为三类:以安徽宁国、亳州等为主的道地产区前胡,以湖南、贵州、重庆等为主的前胡,以及高海拔云南产区的前胡,并确立8个差异性质量标志物.多指标含量测定不同产地前胡的佛手柑内酯、白花前胡甲素、白花前胡乙素、白花前胡素E含量分别为0.016 7～0.131 9 mg/g、6.0 696～16.225 5 mg/g、0.426 5～2.010 0 mg/g、1.340 7～3.115 5 mg/g.本研究所建立的指纹图谱和含量测定方法专属性强,稳定性高,分离度良好,结合化学模式可用于前胡药材的质量评价. |
| Abstract_FL | The HPLC fingerprints and multi-component content determination combined with chemical pattern recognition were developed to evaluate the quality of Peucedani Radix obtained from various regions.The Agilent SB-C18column(4.6 mm × 250 mm,5 μm)was used for the analysis,with a methyl alcohol-0.5％formic acid solution serving as the mobile phase,a volume flow rate of 0.5 mL/min,and a detective wavelength of 321 nm.hierarchical cluster analysis(HCA),principal com-ponent analysis(PCA),and partial least squares discrimination analysis(PLS-DA)were used to analyze the fingerprints.The results showed that nine common peaks were identified out of a total of 21 common peaks that were matched among 16 batches of Peucedani Radix that ranged in similarity from 0.966 to 0.999.Sixteen batches of samples were divided into three groups during chemical pattern recognition analysis,the first group was primarily from Ningguo and Bozhou in the province of Anhui,the second group was primarily from Hunan,Guizhou and Chongqing province,and the third group was the high alti-tude characteristic of the Peucedani Radix.The results also indicated that the content of bergapten,praeruptorin A,praeruptor-in B and praeruptorin E in samples from various regions ranged from 0.016 7 mg/g to 0.131 9 mg/g,6.069 6 mg/g to 16.225 5 mg/g,0.426 5 mg/g to 2.010 0 mg/g,0.013 4 mg/g to 0.031 2 mg/g,respectively.The established method,which has strong specificity,high stability and good separation,can efficiently assess the quality of Peucedani Radix. |
| Author | 王勇庆 沈冰冰 唐雪阳 谢景 张水寒 秦优 陈林 |
| AuthorAffiliation | 湖南省中医药研究院中药资源研究所,长沙 410013;湖南中医药大学药学院,长沙 410208%湖南省中医药研究院中药资源研究所,长沙 410013 |
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| Author_FL | QIN You TANG Xue-yang SHEN Bing-bing ZHANG Shui-han XIE Jing CHEN Lin WANG Yong-qing |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DocumentTitle_FL | Quality evaluation of Peucedani Radix based on HPLC fingerprint combined with chemical pattern recognition and multi-component quantification |
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| Keywords | 含量测定 香豆素 化学模式识别 chemical pattern recognition Peucedani Radix coumarin HPLC fingerprint content determination 前胡 指纹图谱 |
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| Title | 基于HPLC指纹图谱结合化学模式识别以及多指标成分定量评价前胡药材质量 |
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