杜仲皮中木脂素类化学成分及其对高糖诱导的肾小球系膜细胞保护作用研究

R284.1%R285.5; 研究杜仲皮中的木脂素类化学成分,并评价其对高糖诱导的人肾小球系膜细胞的保护作用.将杜仲树皮粉碎后,纯化水提取,采用HPD-100大孔吸附树脂、硅胶、Sephadex LH-20、半制备高效液相色谱等方法进行分离,根据理化性质和波谱学方法鉴定化合物结构,从杜仲皮水提物中分离鉴定了 6个木脂素类化合物,包括caruilignan D(1)、(-)-表松脂素(2)、(+)-松脂素4-O-β-D-吡喃葡萄糖苷(3)、(-)-松脂素4-O-β-D-吡喃葡萄糖苷(4)、(+)-中脂素4-O-β-D-吡喃葡萄糖苷(5)和(+)-中脂素4'-O-β-D-吡喃葡萄糖苷(6...

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Published in天然产物研究与开发 Vol. 35; no. 3; pp. 405 - 419
Main Authors 黄玲, 苏彦红, 蒋跃平, 张燕, 罗荣耀, 张颖帆, 黄琪
Format Journal Article
LanguageChinese
Published 大理大学药学院,大理671000%山东省沂南县湖头镇卫生院,沂南276300%中南大学湘雅医院药学部 28.03.2023
中南大学湘雅医院国家老年疾病临床医学研究中心%中南大学湘雅医院手术室,长沙410008
Subjects
结构鉴定
细胞活力
木脂素
杜仲皮
化学成分
Online AccessGet full text
ISSN1001-6880
DOI10.16333/j.1001-6880.2023.3.006

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Abstract R284.1%R285.5; 研究杜仲皮中的木脂素类化学成分,并评价其对高糖诱导的人肾小球系膜细胞的保护作用.将杜仲树皮粉碎后,纯化水提取,采用HPD-100大孔吸附树脂、硅胶、Sephadex LH-20、半制备高效液相色谱等方法进行分离,根据理化性质和波谱学方法鉴定化合物结构,从杜仲皮水提物中分离鉴定了 6个木脂素类化合物,包括caruilignan D(1)、(-)-表松脂素(2)、(+)-松脂素4-O-β-D-吡喃葡萄糖苷(3)、(-)-松脂素4-O-β-D-吡喃葡萄糖苷(4)、(+)-中脂素4-O-β-D-吡喃葡萄糖苷(5)和(+)-中脂素4'-O-β-D-吡喃葡萄糖苷(6),化合物1为首次从杜仲中分离得到.在高糖诱导异常增殖的肾小球系膜细胞模型中,采用MTT法检测木脂素类化合物干预后的细胞活力.化合物5能剂量依赖性地抑制细胞增殖,对高糖诱导的肾小球系膜细胞具有保护活性.
AbstractList R284.1%R285.5; 研究杜仲皮中的木脂素类化学成分,并评价其对高糖诱导的人肾小球系膜细胞的保护作用.将杜仲树皮粉碎后,纯化水提取,采用HPD-100大孔吸附树脂、硅胶、Sephadex LH-20、半制备高效液相色谱等方法进行分离,根据理化性质和波谱学方法鉴定化合物结构,从杜仲皮水提物中分离鉴定了 6个木脂素类化合物,包括caruilignan D(1)、(-)-表松脂素(2)、(+)-松脂素4-O-β-D-吡喃葡萄糖苷(3)、(-)-松脂素4-O-β-D-吡喃葡萄糖苷(4)、(+)-中脂素4-O-β-D-吡喃葡萄糖苷(5)和(+)-中脂素4'-O-β-D-吡喃葡萄糖苷(6),化合物1为首次从杜仲中分离得到.在高糖诱导异常增殖的肾小球系膜细胞模型中,采用MTT法检测木脂素类化合物干预后的细胞活力.化合物5能剂量依赖性地抑制细胞增殖,对高糖诱导的肾小球系膜细胞具有保护活性.
Author 黄琪
张颖帆
蒋跃平
罗荣耀
苏彦红
黄玲
张燕
AuthorAffiliation 大理大学药学院,大理671000%山东省沂南县湖头镇卫生院,沂南276300%中南大学湘雅医院药学部;中南大学湘雅医院国家老年疾病临床医学研究中心%中南大学湘雅医院手术室,长沙410008
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Author_FL JIANG Yue-ping
LUO Rong-yao
ZHANG Ying-fan
HUANG Qi
ZHANG Yan
HUANG Ling
SU Yan-hong
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Keywords 结构鉴定
细胞活力
木脂素
杜仲皮
化学成分
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Publisher 大理大学药学院,大理671000%山东省沂南县湖头镇卫生院,沂南276300%中南大学湘雅医院药学部
中南大学湘雅医院国家老年疾病临床医学研究中心%中南大学湘雅医院手术室,长沙410008
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Title 杜仲皮中木脂素类化学成分及其对高糖诱导的肾小球系膜细胞保护作用研究
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