基于"有故无殒"理论探究朝鲜淫羊藿对肾阳虚、肾阴虚证模型大鼠肝脏功能的影响
R285.5; 目的 基于"有故无殒"理论观察朝鲜淫羊藿对肾阳虚、肾阴虚及正常大鼠肝脏功能的影响,探究朝鲜淫羊藿致肝损伤与机体证候间的联系.方法 采用随机数字表法将72 只雄性SD大鼠分为正常组、肾阳虚组、肾阴虚组.肾阳虚组大鼠每日肌内注射氢化可的松注射液(20 mg/kg)建立肾阳虚证候模型,肾阴虚组大鼠每日灌胃甲状腺片混悬液(160 mg/kg)建立肾阴虚证候模型,连续14 d.造模成功后将大鼠按随机数字表法分为正常组,朝鲜淫羊藿低、高剂量组,肾阳虚组,肾阳虚+朝鲜淫羊藿低、高剂量组,肾阴虚组,肾阴虚+朝鲜淫羊藿低、高剂量组.药物组动物连续灌胃相应药物,朝鲜淫羊藿低剂量...
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| Published in | 北京中医药大学学报 Vol. 47; no. 11; pp. 1562 - 1572 |
|---|---|
| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
北京中医药大学中医药研究院%北京中医药大学中医药研究院%北京中医药大学中药学院%北京中医药大学中医药研究院
01.11.2024
北京中医药大学中药学院 北京中医药大学中医学院 北京 100029 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1006-2157 |
| DOI | 10.3969/j.issn.1006-2157.2024.11.000 |
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| Abstract | R285.5; 目的 基于"有故无殒"理论观察朝鲜淫羊藿对肾阳虚、肾阴虚及正常大鼠肝脏功能的影响,探究朝鲜淫羊藿致肝损伤与机体证候间的联系.方法 采用随机数字表法将72 只雄性SD大鼠分为正常组、肾阳虚组、肾阴虚组.肾阳虚组大鼠每日肌内注射氢化可的松注射液(20 mg/kg)建立肾阳虚证候模型,肾阴虚组大鼠每日灌胃甲状腺片混悬液(160 mg/kg)建立肾阴虚证候模型,连续14 d.造模成功后将大鼠按随机数字表法分为正常组,朝鲜淫羊藿低、高剂量组,肾阳虚组,肾阳虚+朝鲜淫羊藿低、高剂量组,肾阴虚组,肾阴虚+朝鲜淫羊藿低、高剂量组.药物组动物连续灌胃相应药物,朝鲜淫羊藿低剂量各组剂量为0.26g/kg、朝鲜淫羊藿高剂量各组剂量为0.77 g/kg,其余组予饮用水,每日1次,连续28 d.给药期间监测大鼠体质量变化;给药结束后,采用生化分析仪检测各组大鼠血清碱性磷酸酶(ALP)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、直接胆红素(DBil)、间接胆红素(IBil)、总胆红素(TBil)含量;测量各组大鼠肝脏质量、计算脏体比及脏脑比;HE染色观察各组大鼠肝脏病理变化.结果 与正常组比较,朝鲜淫羊藿高剂量组大鼠在给药第21 天体质量降低,IBil升高(均P<0.05);肾阳虚组大鼠第3～28 天每个测量时间节点体质量均降低,ALP升高,肝脏质量降低(均P<0.05).与肾阳虚组比较,肾阳虚+朝鲜淫羊藿高剂量组大鼠ALP降低(均P<0.05),肾阳虚各给药组大鼠肝组织病理损伤情况有所改善,脂肪空泡情况减少,病理评分有降低趋势.与肾阴虚组比较,肾阴虚+朝鲜淫羊藿高剂量组大鼠IBil升高,脏脑比降低(均P<0.05).结论 在研究剂量范围内,朝鲜淫羊藿对肾阳虚证大鼠肝功能影响较小,对正常大鼠及肾阴虚证大鼠有一定致肝损伤作用,朝鲜淫羊藿致肝损伤与证候间可能存在相关性. |
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| AbstractList | R285.5; 目的 基于"有故无殒"理论观察朝鲜淫羊藿对肾阳虚、肾阴虚及正常大鼠肝脏功能的影响,探究朝鲜淫羊藿致肝损伤与机体证候间的联系.方法 采用随机数字表法将72 只雄性SD大鼠分为正常组、肾阳虚组、肾阴虚组.肾阳虚组大鼠每日肌内注射氢化可的松注射液(20 mg/kg)建立肾阳虚证候模型,肾阴虚组大鼠每日灌胃甲状腺片混悬液(160 mg/kg)建立肾阴虚证候模型,连续14 d.造模成功后将大鼠按随机数字表法分为正常组,朝鲜淫羊藿低、高剂量组,肾阳虚组,肾阳虚+朝鲜淫羊藿低、高剂量组,肾阴虚组,肾阴虚+朝鲜淫羊藿低、高剂量组.药物组动物连续灌胃相应药物,朝鲜淫羊藿低剂量各组剂量为0.26g/kg、朝鲜淫羊藿高剂量各组剂量为0.77 g/kg,其余组予饮用水,每日1次,连续28 d.给药期间监测大鼠体质量变化;给药结束后,采用生化分析仪检测各组大鼠血清碱性磷酸酶(ALP)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、直接胆红素(DBil)、间接胆红素(IBil)、总胆红素(TBil)含量;测量各组大鼠肝脏质量、计算脏体比及脏脑比;HE染色观察各组大鼠肝脏病理变化.结果 与正常组比较,朝鲜淫羊藿高剂量组大鼠在给药第21 天体质量降低,IBil升高(均P<0.05);肾阳虚组大鼠第3～28 天每个测量时间节点体质量均降低,ALP升高,肝脏质量降低(均P<0.05).与肾阳虚组比较,肾阳虚+朝鲜淫羊藿高剂量组大鼠ALP降低(均P<0.05),肾阳虚各给药组大鼠肝组织病理损伤情况有所改善,脂肪空泡情况减少,病理评分有降低趋势.与肾阴虚组比较,肾阴虚+朝鲜淫羊藿高剂量组大鼠IBil升高,脏脑比降低(均P<0.05).结论 在研究剂量范围内,朝鲜淫羊藿对肾阳虚证大鼠肝功能影响较小,对正常大鼠及肾阴虚证大鼠有一定致肝损伤作用,朝鲜淫羊藿致肝损伤与证候间可能存在相关性. |
| Abstract_FL | Objective This study explored the effects of Epimedium koreanum Nakai(EK)on liver function in normal,kidney yang deficiency(KYANGDS),and kidney yin deficiency(KYINDS)rats based on the theory of"You Gu Wu Yun."The study also investigated the connection between EK-induced liver injury and symptoms.Methods Seventy-two male SD rats were divided into normal,KYANGDS,and KYINDS groups using the random number table method.The KYANGDS model was established through intramuscular injection of 20 mg/kg of hydrocortisone,whereas the KYINDS model was established through daily gavage of 160 mg/kg of thyroid tablet suspension for 14 consecutive days.After successful modeling,the rats were divided into the normal,EK low-dose,EK high-dose,KYANGDS,KYANGDS+EK low-dose,KYANGDS+EK high-dose,KYINDS,KYINDS+EK low-dose,and KYINDS+EK high-dose groups using the random number table method.Animals in the drug groups were administered low(0.26 g/kg)and high(0.77 g/kg)EK extract doses through continuous gavage.The other groups received drinking water once a day for 28 consecutive days.Changes in body mass were monitored during the administration period,and serum alkaline phosphatase(ALP),alanine transaminase(ALT),aspartate transaminase(AST),direct bilirubin,indirect bilirubin(IBil),and total bilirubin(TBil)were measured at the end of administration using biochemical analyzers.The liver weight,visceral body ratio,and visceral brain ratio were measured.Hematoxylin and eosin staining were performed to observe the pathological changes in the liver.Results Compared with the normal group,the EK high-dose group showed a decrease in body weight on the 21st day during the drug administration period and an increase in IBil(P<0.05);the KYANGDS group had lower body weight at each measurement time point from the third to the 28th day,higher ALP,and lower liver weight(P<0.05).Compared with the KYANGDS group,the KYANGDS+EK high-dose group had decreased ALP levels(P<0.05).The pathological damage of liver tissue in each KYANGDS administration group improved,the presence of fat vacuoles were reduced,and pathological scores show a decreasing trend.Compared with the KYINDS group,KYINDS+EK high-dose group exhibited a higher IBil level and a lower visceral brain ratio(P<0.05).Conclusion EK has a small effect on the liver function of rats with KYANGDS within the dose range;however,it has a specific hepatogenic impact on normal and KYINDS rats,and EK-induced liver injury and the symptoms may be associated with each other. |
| Author | 翟裕祺 于书伟 杨颂 张林 郭兆娟 彭俙仪 田源 王停 |
| AuthorAffiliation | 北京中医药大学中医学院 北京 100029;北京中医药大学中医药研究院%北京中医药大学中医药研究院%北京中医药大学中药学院%北京中医药大学中医药研究院;北京中医药大学中药学院 |
| AuthorAffiliation_xml | – name: 北京中医药大学中医学院 北京 100029;北京中医药大学中医药研究院%北京中医药大学中医药研究院%北京中医药大学中药学院%北京中医药大学中医药研究院;北京中医药大学中药学院 |
| Author_FL | TIAN Yuan GUO Zhaojuan ZHANG Lin YU Shuwei ZHAI Yuqi YANG Song PENG Xiyi WANG Ting |
| Author_FL_xml | – sequence: 1 fullname: PENG Xiyi – sequence: 2 fullname: ZHANG Lin – sequence: 3 fullname: ZHAI Yuqi – sequence: 4 fullname: YU Shuwei – sequence: 5 fullname: TIAN Yuan – sequence: 6 fullname: GUO Zhaojuan – sequence: 7 fullname: YANG Song – sequence: 8 fullname: WANG Ting |
| Author_xml | – sequence: 1 fullname: 彭俙仪 – sequence: 2 fullname: 张林 – sequence: 3 fullname: 翟裕祺 – sequence: 4 fullname: 于书伟 – sequence: 5 fullname: 田源 – sequence: 6 fullname: 郭兆娟 – sequence: 7 fullname: 杨颂 – sequence: 8 fullname: 王停 |
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| DocumentTitle_FL | The effects of Epimedium koreanum Nakai on liver function in rats with kidney yang deficiency and kidney yin deficiency pattern models based on the"You Gu Wu Yun"theory |
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| Keywords | 有故无殒 大鼠 liver injury Epimedium koreanum Nakai kidney yin deficiency pattern 肾阳虚证 肾阴虚证 You Gu Wu Yun 朝鲜淫羊藿 rats 肝损伤 kidney yang deficiency pattern |
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| PublicationTitle | 北京中医药大学学报 |
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| Snippet | R285.5; 目的 基于"有故无殒"理论观察朝鲜淫羊藿对肾阳虚、肾阴虚及正常大鼠肝脏功能的影响,探究朝鲜淫羊藿致肝损伤与机体证候间的联系.方法 采用随机数字表法将72 只雄... |
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| Title | 基于"有故无殒"理论探究朝鲜淫羊藿对肾阳虚、肾阴虚证模型大鼠肝脏功能的影响 |
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