黄芪皂苷Ⅰ通过抑制足细胞焦亡干预糖尿病肾脏疾病的机制研究
R285.5; 目的 探讨黄芪活性成分黄芪皂苷Ⅰ抑制足细胞损伤、改善糖尿病肾脏疾病的作用机制.方法 60只雄性db/db小鼠按体质量随机分为模型组、黄芪皂苷Ⅰ低剂量组(10 mg/kg)、黄芪皂苷Ⅰ中剂量组(20 mg/kg)、黄芪皂苷Ⅰ高剂量组(40 mg/kg)及缬沙坦组(10 mg/kg),每组12只;12只db/db同窝对照db/m小鼠作为对照组.灌胃给药8周.透射电子显微镜观察肾脏超微结构;免疫组织化学法、蛋白质印迹法检测肾脏足细胞标志物肾病蛋白(nephrin)的表达情况;酶联免疫吸附测定法检测小鼠血清白细胞介素-1β(IL-1β)、白细胞介素-18(IL-18)含量;蛋白质印迹法...
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| Published in | 北京中医药大学学报 Vol. 47; no. 10; pp. 1408 - 1415 |
|---|---|
| Main Authors | , , , , , , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
河南中医药大学中医药科学院 郑州 450046
01.10.2024
河南中医药大学豫药全产业链研发河南省协同创新中心%河南中医药大学中医学院 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1006-2157 |
| DOI | 10.3969/j.issn.1006-2157.2024.10.011 |
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| Abstract | R285.5; 目的 探讨黄芪活性成分黄芪皂苷Ⅰ抑制足细胞损伤、改善糖尿病肾脏疾病的作用机制.方法 60只雄性db/db小鼠按体质量随机分为模型组、黄芪皂苷Ⅰ低剂量组(10 mg/kg)、黄芪皂苷Ⅰ中剂量组(20 mg/kg)、黄芪皂苷Ⅰ高剂量组(40 mg/kg)及缬沙坦组(10 mg/kg),每组12只;12只db/db同窝对照db/m小鼠作为对照组.灌胃给药8周.透射电子显微镜观察肾脏超微结构;免疫组织化学法、蛋白质印迹法检测肾脏足细胞标志物肾病蛋白(nephrin)的表达情况;酶联免疫吸附测定法检测小鼠血清白细胞介素-1β(IL-1β)、白细胞介素-18(IL-18)含量;蛋白质印迹法检测肾脏组织NOD样受体热蛋白结构域相关蛋白3(NLRP3)、胱天蛋白酶-1(Caspase-1)、消皮素D(GSDMD)蛋白表达.结果 与对照组比较,模型组小鼠肾小球出现明显的足细胞丢失、足突融合等现象;肾脏nephrin蛋白表达下降(P＜0.05);血清IL-1β、IL-18含量升高(均P＜0.05);NLRP3、裂解型Caspase-1(Cleaved-Caspase-1)、GSDMD-N蛋白表达升高(均P＜0.05).与模型组比较,黄芪皂苷Ⅰ给药组肾脏病理损伤得到缓解;Nephein蛋白表达升高(P＜0.05);血清IL-1β、IL-18含量下降(均 P＜0.05);NLRP3、Cleaved-Caspase-1、GSDMD-N 蛋白表达下降(均 P＜0.05).结论 黄芪皂苷Ⅰ可能通过抑制细胞焦亡、改善足细胞损伤发挥干预糖尿病肾脏疾病的作用. |
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| AbstractList | R285.5; 目的 探讨黄芪活性成分黄芪皂苷Ⅰ抑制足细胞损伤、改善糖尿病肾脏疾病的作用机制.方法 60只雄性db/db小鼠按体质量随机分为模型组、黄芪皂苷Ⅰ低剂量组(10 mg/kg)、黄芪皂苷Ⅰ中剂量组(20 mg/kg)、黄芪皂苷Ⅰ高剂量组(40 mg/kg)及缬沙坦组(10 mg/kg),每组12只;12只db/db同窝对照db/m小鼠作为对照组.灌胃给药8周.透射电子显微镜观察肾脏超微结构;免疫组织化学法、蛋白质印迹法检测肾脏足细胞标志物肾病蛋白(nephrin)的表达情况;酶联免疫吸附测定法检测小鼠血清白细胞介素-1β(IL-1β)、白细胞介素-18(IL-18)含量;蛋白质印迹法检测肾脏组织NOD样受体热蛋白结构域相关蛋白3(NLRP3)、胱天蛋白酶-1(Caspase-1)、消皮素D(GSDMD)蛋白表达.结果 与对照组比较,模型组小鼠肾小球出现明显的足细胞丢失、足突融合等现象;肾脏nephrin蛋白表达下降(P＜0.05);血清IL-1β、IL-18含量升高(均P＜0.05);NLRP3、裂解型Caspase-1(Cleaved-Caspase-1)、GSDMD-N蛋白表达升高(均P＜0.05).与模型组比较,黄芪皂苷Ⅰ给药组肾脏病理损伤得到缓解;Nephein蛋白表达升高(P＜0.05);血清IL-1β、IL-18含量下降(均 P＜0.05);NLRP3、Cleaved-Caspase-1、GSDMD-N 蛋白表达下降(均 P＜0.05).结论 黄芪皂苷Ⅰ可能通过抑制细胞焦亡、改善足细胞损伤发挥干预糖尿病肾脏疾病的作用. |
| Abstract_FL | Objective To investigate the mechanism of astragaloside Ⅰ,the active constituent of milkvetch root,in inhibiting podocyte injury and improving diabetic kidney disease.Methods According to the body weight,60 male db/db mice were randomly divided into the model group,astragaloside Ⅰ low-dose group(10 mg/kg),astragaloside Ⅰ medium-dose group(20 mg/kg),astragaloside Ⅰ high-dose group(40 mg/kg),and valsartan group(10mg/kg),with 12 mice per group.Twelve db/db littermate control db/m mice were used as the control group.The drug was administered by gavage for 8 weeks.Transmission electron microscope was used to observe the ultrastructure of the kidney;immunohistochemistry and Western blotting were used to detect the expression of nephrotic protein(nephrin),a marker of renal podocytes;enzyme-linked immunosorbent assay was used to detect the contents of interleukin-1β(IL-1β)and interleukin-18(IL-18)in the serum of mice;Western blotting was used to detect the protein expressions of NOD-like receptor thermoprotein domain-related protein 3(NLRP3),cysteinyl aspartate specific proteinase 1(Caspase-1),and Gasdermin D(GSDMD)in kidney tissue.Results Compared with the control group,the glomeruli of the model group showed obvious podocyte loss and foot process fusion;the protein expression of nephrin was decreased(P＜0.05);the contents of IL-1 β and IL-18 in serum were increased(P＜0.05);the protein expressions of NLRP3,Cleaved-Caspase-1,and GSDMD-N were increased(P＜0.05).Compared with the model group,the renal pathological damage in the astragaloside Ⅰ administration groups were alleviated;the protein expression of nephrin was increased(P＜0.05);the contents of IL-1β and IL-18 in serum were decreased(P＜0.05);the protein expressions of NLRP3,Cleaved-Caspase-1,and GSDMD-N were decreased(P＜0.05).Conclusion Astragaloside Ⅰ may play a role in intervening diabetic kidney disease by inhibiting pyroptosis and improving podocyte injury. |
| Author | 谢治深 徐江雁 段亚飞 任心棋 张效威 石贤聪 张振强 古豫蕾 吕明真 赵靓 苗晋鑫 |
| AuthorAffiliation | 河南中医药大学中医药科学院 郑州 450046;河南中医药大学豫药全产业链研发河南省协同创新中心%河南中医药大学中医学院 |
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| Author_FL | XIE Zhishen ZHANG Zhenqiang MIAO Jinxin LYU Mingzhen REN Xinqi XU Jiangyan DUAN Yafei SHI Xiancong ZHAO Liang ZHANG Xiaowei GU Yulei |
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| Keywords | diabetic kidney disease 细胞焦亡 pyroptosis milkvetch root 黄芪 podocytes 糖尿病肾脏疾病 足细胞 小鼠 astragaloside mice 黄芪皂苷 |
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| PublicationYear | 2024 |
| Publisher | 河南中医药大学中医药科学院 郑州 450046 河南中医药大学豫药全产业链研发河南省协同创新中心%河南中医药大学中医学院 |
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| Title | 黄芪皂苷Ⅰ通过抑制足细胞焦亡干预糖尿病肾脏疾病的机制研究 |
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