油葵苗期抗旱相关性状的QTL定位及候选基因筛选
Q343.1+7%S565.5; 干旱是限制向日葵生长发育的重要因素之一.为探究向日葵苗期抗旱性分子机制,该研究以向日葵K55与K58杂交构建的150个F7重组自交系群体为材料,对其在正常浇水和干旱胁迫两种水分处理条件下的叶片相对电导率、叶绿素含量、叶面积、叶片相对含水量、根长进行表型测定,利用前期建立的SNP、SSR分子标记遗传连锁图谱,通过复合区间作图法对5个抗旱相关的性状进行QTL定位.结果表明:(1)共定位到向日葵QTL位点11个,其中正常浇水条件下5个,干旱胁迫条件下6个,表型贡献率为0.768％～7.547％,且5号连锁群上定位到的QTL位点最多(3个).(2)QTL置信区间内共筛...
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| Published in | 西北植物学报 Vol. 42; no. 8; pp. 1420 - 1429 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
内蒙古农业大学农学院,呼和浩特010019
2022
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1000-4025 |
| DOI | 10.7606/j.issn.1000-4025.2022.08.1420 |
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| Abstract | Q343.1+7%S565.5; 干旱是限制向日葵生长发育的重要因素之一.为探究向日葵苗期抗旱性分子机制,该研究以向日葵K55与K58杂交构建的150个F7重组自交系群体为材料,对其在正常浇水和干旱胁迫两种水分处理条件下的叶片相对电导率、叶绿素含量、叶面积、叶片相对含水量、根长进行表型测定,利用前期建立的SNP、SSR分子标记遗传连锁图谱,通过复合区间作图法对5个抗旱相关的性状进行QTL定位.结果表明:(1)共定位到向日葵QTL位点11个,其中正常浇水条件下5个,干旱胁迫条件下6个,表型贡献率为0.768％～7.547％,且5号连锁群上定位到的QTL位点最多(3个).(2)QTL置信区间内共筛选到62个与干旱相关的候选基因,包括位于qLA-8-1上的rna23019、rna23004、rna22661、rna22193、rna23294、rna22783和位于qCC-13-1上的rna40140,这些基因可作为后续基因克隆及功能研究的重点候选基因.该研究结果为向日葵抗旱性研究及其遗传改良奠定了基础. |
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| AbstractList | Q343.1+7%S565.5; 干旱是限制向日葵生长发育的重要因素之一.为探究向日葵苗期抗旱性分子机制,该研究以向日葵K55与K58杂交构建的150个F7重组自交系群体为材料,对其在正常浇水和干旱胁迫两种水分处理条件下的叶片相对电导率、叶绿素含量、叶面积、叶片相对含水量、根长进行表型测定,利用前期建立的SNP、SSR分子标记遗传连锁图谱,通过复合区间作图法对5个抗旱相关的性状进行QTL定位.结果表明:(1)共定位到向日葵QTL位点11个,其中正常浇水条件下5个,干旱胁迫条件下6个,表型贡献率为0.768％～7.547％,且5号连锁群上定位到的QTL位点最多(3个).(2)QTL置信区间内共筛选到62个与干旱相关的候选基因,包括位于qLA-8-1上的rna23019、rna23004、rna22661、rna22193、rna23294、rna22783和位于qCC-13-1上的rna40140,这些基因可作为后续基因克隆及功能研究的重点候选基因.该研究结果为向日葵抗旱性研究及其遗传改良奠定了基础. |
| Author | 李丹丹 苏飞燕 王艳霞 周佳岭 石慧敏 侯建华 |
| AuthorAffiliation | 内蒙古农业大学农学院,呼和浩特010019 |
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| Author_FL | LI Dandan SHI Huimin HOU Jianhua WANG Yanxia SU Feiyan ZHOU Jialing |
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| Keywords | 苗期 候选基因筛选 向日葵 干旱胁迫 QTL定位 |
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| Snippet | Q343.1+7%S565.5; 干旱是限制向日葵生长发育的重要因素之一.为探究向日葵苗期抗旱性分子机制,该研究以向日葵K55与K58杂交构建的150个F7重组自交系群体为材料,对其在正常浇... |
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| Title | 油葵苗期抗旱相关性状的QTL定位及候选基因筛选 |
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