宏基因组二代测序在伴有中枢神经系统受累儿童噬血细胞综合征中的应用价值

目的 探究宏基因组二代测序(metagenomic next-generation sequencing,mNGS)技术检测伴有中枢神经系统受累的噬血细胞综合征患儿颅内EB病毒(Epstein-Barr virus,EBV)感染的应用价值及临床意义.方法 回顾性分析30例伴有中枢神经系统受累的噬血细胞综合征患儿的脑脊液mNGS结果,与脑脊液EBV-DNA定性检测、血清EBV抗体谱检测结果进行比较,以治疗前后血清EBV-DNA拷贝数变化反映针对性治疗效果.结果 脑脊液mNGS EBV检测阳性率为100％(30/30),高于脑脊液EBV-DNA定性检测阳性率(10％,3/30;P<0.001...

Full description

Saved in:
Bibliographic Details
Published in中国当代儿科杂志 Vol. 24; no. 11; pp. 1226 - 1230
Main Authors 张海洋, 唐茂婷, 卿露, 李德渊, 乔莉娜
Format Journal Article
LanguageChinese
Published 四川大学华西第二医院儿童重症医学科/出生缺陷与相关妇儿疾病教育部重点实验室,四川成都 610041 01.11.2022
Subjects
儿童
EB病毒
宏基因组二代测序
噬血细胞综合征
Online AccessGet full text
ISSN1008-8830
DOI10.7499/j.issn.1008-8830.2206118

Cover

Abstract 目的 探究宏基因组二代测序(metagenomic next-generation sequencing,mNGS)技术检测伴有中枢神经系统受累的噬血细胞综合征患儿颅内EB病毒(Epstein-Barr virus,EBV)感染的应用价值及临床意义.方法 回顾性分析30例伴有中枢神经系统受累的噬血细胞综合征患儿的脑脊液mNGS结果,与脑脊液EBV-DNA定性检测、血清EBV抗体谱检测结果进行比较,以治疗前后血清EBV-DNA拷贝数变化反映针对性治疗效果.结果 脑脊液mNGS EBV检测阳性率为100％(30/30),高于脑脊液EBV-DNA定性检测阳性率(10％,3/30;P<0.001),与血清EBV抗体谱检测阳性率(93％,28/30)比较差异无统计学意义(P>0.05).中位mNGS EBV检出序列数为2400,治疗前血清EBV-DNA拷贝数与EBV检出序列数呈中度正相关(rs=0.693,P<0.001).多元线性回归分析结果显示,治疗前血清EBV-DNA拷贝数越高,脑脊液mNGS EBV检出序列数越高(P<0.05).结论 EBV相关噬血细胞综合征容易诱发EBV感染引起的病毒性脑炎,mNGS可以显著提高脑脊液EBV检测的阳性率,协助临床诊断.
AbstractList 目的 探究宏基因组二代测序(metagenomic next-generation sequencing,mNGS)技术检测伴有中枢神经系统受累的噬血细胞综合征患儿颅内EB病毒(Epstein-Barr virus,EBV)感染的应用价值及临床意义.方法 回顾性分析30例伴有中枢神经系统受累的噬血细胞综合征患儿的脑脊液mNGS结果,与脑脊液EBV-DNA定性检测、血清EBV抗体谱检测结果进行比较,以治疗前后血清EBV-DNA拷贝数变化反映针对性治疗效果.结果 脑脊液mNGS EBV检测阳性率为100％(30/30),高于脑脊液EBV-DNA定性检测阳性率(10％,3/30;P<0.001),与血清EBV抗体谱检测阳性率(93％,28/30)比较差异无统计学意义(P>0.05).中位mNGS EBV检出序列数为2400,治疗前血清EBV-DNA拷贝数与EBV检出序列数呈中度正相关(rs=0.693,P<0.001).多元线性回归分析结果显示,治疗前血清EBV-DNA拷贝数越高,脑脊液mNGS EBV检出序列数越高(P<0.05).结论 EBV相关噬血细胞综合征容易诱发EBV感染引起的病毒性脑炎,mNGS可以显著提高脑脊液EBV检测的阳性率,协助临床诊断.
Author 乔莉娜
卿露
唐茂婷
张海洋
李德渊
AuthorAffiliation 四川大学华西第二医院儿童重症医学科/出生缺陷与相关妇儿疾病教育部重点实验室,四川成都 610041
AuthorAffiliation_xml – name: 四川大学华西第二医院儿童重症医学科/出生缺陷与相关妇儿疾病教育部重点实验室,四川成都 610041
Author_FL ZHANG Hai-Yang
QIAO Li-Na
TANG Mao-Ting
QING Lu
LI De-Yuan
Author_FL_xml – sequence: 1
  fullname: ZHANG Hai-Yang
– sequence: 2
  fullname: TANG Mao-Ting
– sequence: 3
  fullname: QING Lu
– sequence: 4
  fullname: LI De-Yuan
– sequence: 5
  fullname: QIAO Li-Na
Author_xml – sequence: 1
  fullname: 张海洋
– sequence: 2
  fullname: 唐茂婷
– sequence: 3
  fullname: 卿露
– sequence: 4
  fullname: 李德渊
– sequence: 5
  fullname: 乔莉娜
BookMark eNo9kM1LAkEAxedgkFr_Q9cOu83sjLMzx5C-QOjiXcZ2R7JYoSUCT0YSRcF6EKIS7QNB6OuQUexI_TM7jn9GRtHpweP93oOXAamgFvgALCBou4Tzpaq9HYaBjSBkFmMY2o4DKUIsBdL_3izIhGEVwhwjHKeB1M-R7sX6-saoZhKfJ-p-_Ham40h3BsloOO6cJh9P4-6d6XeNisyrMqqnowszfNHNL_PQ15ePk9uGUceTo2lgpFsn-vNwipirpo7bpj1I1LtujObAjBS7oT__p1lQXF0p5tetwubaRn65YIUIEma5VCAmIJOwTBCnkpexFL7nCcYxdyjhgvqY0y0hqet7jktyGJVdmvMpIZBCnAWLv7UHIpAiqJSqtf29YDpYqlc8z9-p1x3oOOjnDPwN032BdA
ContentType Journal Article
Copyright Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
Copyright_xml – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
DBID 2B.
4A8
92I
93N
PSX
TCJ
DOI 10.7499/j.issn.1008-8830.2206118
DatabaseName Wanfang Data Journals - Hong Kong
WANFANG Data Centre
Wanfang Data Journals
万方数据期刊 - 香港版
China Online Journals (COJ)
China Online Journals (COJ)
DatabaseTitleList
DeliveryMethod fulltext_linktorsrc
DocumentTitle_FL Value of metagenomic next-generation sequencing in children with hemophagocytic syndrome with central nervous system involvement
EndPage 1230
ExternalDocumentID zgddekzz202211008
GrantInformation_xml – fundername: 国家重点研发计划
  funderid: (2021YFC2701705)
GroupedDBID ---
-05
2B.
4A8
92F
92I
93N
ABJNI
ACGFS
ALMA_UNASSIGNED_HOLDINGS
CCEZO
CIEJG
CW9
EMOBN
F5P
PSX
RPM
TCJ
TGQ
U1G
U5O
ID FETCH-LOGICAL-s1048-76a18a08f0b4196f9b3faedda89392649a6e396caf67ed274531b765e6440603
ISSN 1008-8830
IngestDate Thu May 29 03:59:54 EDT 2025
IsPeerReviewed false
IsScholarly true
Issue 11
Keywords 儿童
EB病毒
宏基因组二代测序
噬血细胞综合征
Language Chinese
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-s1048-76a18a08f0b4196f9b3faedda89392649a6e396caf67ed274531b765e6440603
PageCount 5
ParticipantIDs wanfang_journals_zgddekzz202211008
PublicationCentury 2000
PublicationDate 2022-11-01
PublicationDateYYYYMMDD 2022-11-01
PublicationDate_xml – month: 11
  year: 2022
  text: 2022-11-01
  day: 01
PublicationDecade 2020
PublicationTitle 中国当代儿科杂志
PublicationTitle_FL Chinese Journal of Contemporary Pediatrics
PublicationYear 2022
Publisher 四川大学华西第二医院儿童重症医学科/出生缺陷与相关妇儿疾病教育部重点实验室,四川成都 610041
Publisher_xml – name: 四川大学华西第二医院儿童重症医学科/出生缺陷与相关妇儿疾病教育部重点实验室,四川成都 610041
SSID ssj0058493
ssib051368748
ssib001103687
ssib046626328
ssib002262955
Score 2.3549016
Snippet 目的 探究宏基因组二代测序(metagenomic next-generation sequencing,mNGS)技术检测伴有中枢神经系统受累的噬血细胞综合征患儿颅内EB病毒(Epstein-Barr virus,EBV)感染的...
SourceID wanfang
SourceType Aggregation Database
StartPage 1226
Title 宏基因组二代测序在伴有中枢神经系统受累儿童噬血细胞综合征中的应用价值
URI https://d.wanfangdata.com.cn/periodical/zgddekzz202211008
Volume 24
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
journalDatabaseRights – providerCode: PRVAQN
  databaseName: PubMed Central
  issn: 1008-8830
  databaseCode: RPM
  dateStart: 20160101
  customDbUrl:
  isFulltext: true
  dateEnd: 99991231
  titleUrlDefault: https://www.ncbi.nlm.nih.gov/pmc/
  omitProxy: true
  ssIdentifier: ssj0058493
  providerName: National Library of Medicine
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Na9RAFA-1vQgiiorfFHFOsnWTTCYzxyS7SxH0VKG3kmx2Kwor2Payp4pFFIX1UBC1tH5QKPh1sKLsFv1nNk3_DN97meymukL10DC8eXlfv9fMm9mZxDAuw5jSgLLWKTlxHJd4GDsl-FMleFiGzagRW3XaTXj9hpi-ya_NOrNjE0cKu5aWFqOpenvkuZL_QRVogCuekv0HZAdCgQBtwBeugDBcD4QxqzrMqzJZw4aqMd-jhs-8Mqu6zPeZ5KzKkS4DakCXzaqC-Q6TPjJjV3Z7wDxJPAHzOfIARSqiSOZViFJlnoWSPQfbWkWNGja2M4oigUBXLlE48zIKiCJmz0cJqFQxDwwD-SZuudACBVKkPVQBJiFzmUlJNoPLZsEwlymPPCV3FCcKz92B213SXmb6M9638-gNJFDQfGrAVdmFWBXNBrEmxaHCpEXM6GOes0QIKPYUYFQr0HvpF1nAMvAEPQy0FE8Bb5FFVkijQgh8a9iTaa7qGGj5IMgrrt3AtN_ct3aj3VMZ3i7KQKUc_cFGhXlCa0XZErHRDgcEzyCBHLwizIqQs0aBqph0URRi4GJ7eNdAVx5KK0sLlxKXMFNZMgVEIS3oJeEkKRsQJ0oC6WDOoUyRa8ktUQLDow1wKGyUahhzCwJKRtqUHwVrsUvp_yjMPHAw9xooEC4rGBVJgUmJgJJMv3JF4NsRzcJgj1t_pNS_C-pqIDvRnz_1zMLYblqWKNSJUPKVR9UgLleKahDUMTXQMWVZUDrrWmP_G97b81Bj3Gm3MUPwDY7ykDFhQZFS_m3xEfpsIYurL8JSw2PlXOCbpYarCY6J3HzwoypMNVR23kiblG1sRHuv_s1aOsrYaoat-ULVPXPMOKqny5Ne9uw7boy1b50wmsmnTrLRTV69Tnsr_e7Tfu_d7tcnSbeTrG31d7Z31x73v3_cXX-bbq6nvU76pZf2NpLO83T7c7LyM32_mbz4sPdmOe093HsADDvJs0fJj_twS_pyJemupqtb_d63ZHnnpDFTq84E0yX9vZjSggmFSMkVoSnDsmyWIw6FRVNFdjNsxHEIczIFEz8VioatRD1sChdGIZdD_RG5woHBCqY1ZfuUMd6622qcNiabIX41pB463I65sHgUWsp06_XIoQlk44xxSYdlTg8HC3N_oHj2IEznjMPDB8N5Y3zx3lLjAkxzFqOLBP4vLgsBWw
linkProvider National Library of Medicine
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E5%AE%8F%E5%9F%BA%E5%9B%A0%E7%BB%84%E4%BA%8C%E4%BB%A3%E6%B5%8B%E5%BA%8F%E5%9C%A8%E4%BC%B4%E6%9C%89%E4%B8%AD%E6%9E%A2%E7%A5%9E%E7%BB%8F%E7%B3%BB%E7%BB%9F%E5%8F%97%E7%B4%AF%E5%84%BF%E7%AB%A5%E5%99%AC%E8%A1%80%E7%BB%86%E8%83%9E%E7%BB%BC%E5%90%88%E5%BE%81%E4%B8%AD%E7%9A%84%E5%BA%94%E7%94%A8%E4%BB%B7%E5%80%BC&rft.jtitle=%E4%B8%AD%E5%9B%BD%E5%BD%93%E4%BB%A3%E5%84%BF%E7%A7%91%E6%9D%82%E5%BF%97&rft.au=%E5%BC%A0%E6%B5%B7%E6%B4%8B&rft.au=%E5%94%90%E8%8C%82%E5%A9%B7&rft.au=%E5%8D%BF%E9%9C%B2&rft.au=%E6%9D%8E%E5%BE%B7%E6%B8%8A&rft.date=2022-11-01&rft.pub=%E5%9B%9B%E5%B7%9D%E5%A4%A7%E5%AD%A6%E5%8D%8E%E8%A5%BF%E7%AC%AC%E4%BA%8C%E5%8C%BB%E9%99%A2%E5%84%BF%E7%AB%A5%E9%87%8D%E7%97%87%E5%8C%BB%E5%AD%A6%E7%A7%91%2F%E5%87%BA%E7%94%9F%E7%BC%BA%E9%99%B7%E4%B8%8E%E7%9B%B8%E5%85%B3%E5%A6%87%E5%84%BF%E7%96%BE%E7%97%85%E6%95%99%E8%82%B2%E9%83%A8%E9%87%8D%E7%82%B9%E5%AE%9E%E9%AA%8C%E5%AE%A4%2C%E5%9B%9B%E5%B7%9D%E6%88%90%E9%83%BD+610041&rft.issn=1008-8830&rft.volume=24&rft.issue=11&rft.spage=1226&rft.epage=1230&rft_id=info:doi/10.7499%2Fj.issn.1008-8830.2206118&rft.externalDocID=zgddekzz202211008
thumbnail_s http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fzgddekzz%2Fzgddekzz.jpg