WT1多肽疫苗对荷人单核细胞白血病SCID小鼠的抗肿瘤免疫效应

R557; 目的 研究WT1多肽疫苗对荷人单核细胞白血病重度联合免疫缺陷(SCID)小鼠的抗肿瘤免疫效应.方法 对SCID小鼠腹腔注射人外周血淋巴细胞(PBL)后24 h,皮下接种THP1细胞,建立SCID小鼠荷人单核细胞白血病模型,随机分组,每组8只.空白对照组的疫苗成分为不完全弗氏佐剂,辅助T细胞表位组的疫苗成分为辅助T细胞表位和不完全弗氏佐剂,WT1多肽组的疫苗成分为WT1多肽、辅助T细胞表位和不完全弗氏佐剂.当肿瘤体积约为100 mm3时,开始腹腔注射疫苗成分,疫苗接种14d后处死SCID小鼠.采用乳酸脱氢酶法检测小鼠脾细胞特异性CTL杀伤活性,肿瘤组织HE染色后镜下观察组织学特点,利...

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Published in西安交通大学学报（医学版） Vol. 40; no. 5; pp. 690 - 695
Main Authors 李静, 张王刚, 钟波, 白菊, 刘海燕, 王慧渊, 耿妍
Format Journal Article
LanguageChinese
Published 西安交通大学第二附属医院儿科,陕西西安,710004%西安交通大学第二附属医院血液内科,陕西西安,710004 05.09.2019
Subjects
WT1多肽疫苗
抗肿瘤免疫效应
SCID小鼠
单核细胞白血病
Online AccessGet full text
ISSN1671-8259
DOI10.7652/jdyxb201905005

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Abstract R557; 目的 研究WT1多肽疫苗对荷人单核细胞白血病重度联合免疫缺陷(SCID)小鼠的抗肿瘤免疫效应.方法 对SCID小鼠腹腔注射人外周血淋巴细胞(PBL)后24 h,皮下接种THP1细胞,建立SCID小鼠荷人单核细胞白血病模型,随机分组,每组8只.空白对照组的疫苗成分为不完全弗氏佐剂,辅助T细胞表位组的疫苗成分为辅助T细胞表位和不完全弗氏佐剂,WT1多肽组的疫苗成分为WT1多肽、辅助T细胞表位和不完全弗氏佐剂.当肿瘤体积约为100 mm3时,开始腹腔注射疫苗成分,疫苗接种14d后处死SCID小鼠.采用乳酸脱氢酶法检测小鼠脾细胞特异性CTL杀伤活性,肿瘤组织HE染色后镜下观察组织学特点,利用流式细胞仪检测外周血CD3+/CD4+T细胞、CD3+/CD8+T细胞及CD4+ CD25+T细胞水平,应用ELISA方法检测血清中免疫球蛋白、IL-2、IFN-γ、TGF-β及IL-10水平.结果 实验成功建立荷人单核细胞白血病SCID小鼠模型,各组中所有动物均成瘤;WT1组小鼠脾细胞对THP1细胞的CTL活性高于辅助T细胞表位组和空白对照组(P＜0.05);WT1组小鼠的肿瘤平均体质量及体积均明显低于辅助T细胞表位组和空白对照组(P＜0.05);WT1组肿瘤组织HE染色显示肿瘤细胞变性坏死,存活的肿瘤细胞减少;WT1组小鼠外周血CD3+/CD4+T细胞、CD3+/CD8+T细胞、IgG、IFN γ和IL-2水平均明显高于辅助T细胞表位组和空白对照组(P＜0.05);外周血CD4+/CD25+ Treg细胞、TGF-β和IL-10水平均明显低于辅助T细胞表位组和空白对照组(P＜0.05).结论 WT1多肽疫苗可在荷人单核细胞白血病SCID小鼠体内产生抗肿瘤免疫效应,有效杀伤白血病细胞.
AbstractList R557; 目的 研究WT1多肽疫苗对荷人单核细胞白血病重度联合免疫缺陷(SCID)小鼠的抗肿瘤免疫效应.方法 对SCID小鼠腹腔注射人外周血淋巴细胞(PBL)后24 h,皮下接种THP1细胞,建立SCID小鼠荷人单核细胞白血病模型,随机分组,每组8只.空白对照组的疫苗成分为不完全弗氏佐剂,辅助T细胞表位组的疫苗成分为辅助T细胞表位和不完全弗氏佐剂,WT1多肽组的疫苗成分为WT1多肽、辅助T细胞表位和不完全弗氏佐剂.当肿瘤体积约为100 mm3时,开始腹腔注射疫苗成分,疫苗接种14d后处死SCID小鼠.采用乳酸脱氢酶法检测小鼠脾细胞特异性CTL杀伤活性,肿瘤组织HE染色后镜下观察组织学特点,利用流式细胞仪检测外周血CD3+/CD4+T细胞、CD3+/CD8+T细胞及CD4+ CD25+T细胞水平,应用ELISA方法检测血清中免疫球蛋白、IL-2、IFN-γ、TGF-β及IL-10水平.结果 实验成功建立荷人单核细胞白血病SCID小鼠模型,各组中所有动物均成瘤;WT1组小鼠脾细胞对THP1细胞的CTL活性高于辅助T细胞表位组和空白对照组(P＜0.05);WT1组小鼠的肿瘤平均体质量及体积均明显低于辅助T细胞表位组和空白对照组(P＜0.05);WT1组肿瘤组织HE染色显示肿瘤细胞变性坏死,存活的肿瘤细胞减少;WT1组小鼠外周血CD3+/CD4+T细胞、CD3+/CD8+T细胞、IgG、IFN γ和IL-2水平均明显高于辅助T细胞表位组和空白对照组(P＜0.05);外周血CD4+/CD25+ Treg细胞、TGF-β和IL-10水平均明显低于辅助T细胞表位组和空白对照组(P＜0.05).结论 WT1多肽疫苗可在荷人单核细胞白血病SCID小鼠体内产生抗肿瘤免疫效应,有效杀伤白血病细胞.
Author 钟波
耿妍
白菊
王慧渊
李静
刘海燕
张王刚
AuthorAffiliation 西安交通大学第二附属医院儿科,陕西西安,710004%西安交通大学第二附属医院血液内科,陕西西安,710004
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Author_FL BAI Ju
ZHONG Bo
LIU Hai-yan
LI Jing
ZHANG Wang-gang
WANG Hui-yuan
GENG Yan
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Keywords WT1多肽疫苗
抗肿瘤免疫效应
SCID小鼠
单核细胞白血病
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Snippet R557; 目的 研究WT1多肽疫苗对荷人单核细胞白血病重度联合免疫缺陷(SCID)小鼠的抗肿瘤免疫效应.方法 对SCID小鼠腹腔注射人外周血淋巴细胞(PBL)后24 h,皮下接种THP1细胞,建...
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StartPage 690
Title WT1多肽疫苗对荷人单核细胞白血病SCID小鼠的抗肿瘤免疫效应
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