角质酶的异源表达和超声辅助下降解PET塑料
Q814; 聚对苯二甲酸乙二醇酯(PET)是一种典型的线性热塑性树脂材料,被广泛用于制造纤维、薄膜和饮料瓶.但是,PET为人类带来生活便利的同时也为全球环境造成不利影响,因为其难被降解,造成了大量的白色污染.本研究利用Escherichia.coli BL21(DE3)成功表达重组角质酶LCC,最优表达条件为:诱导剂异丙基-β-D-硫代半乳糖苷(IPTG)浓度0.2 mmol/L,诱导温度16℃,诱导时间20 h.经Ni离子亲和层析纯化后,LCC酶活为320 U/mg.采用超声辅助LCC降解PET,对其超声条件进行优化,温度为65℃,pH=8.0,超声功率为25 W,超声时间为5 h时,LCC...
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| Published in | 华南理工大学学报（自然科学版） Vol. 50; no. 1; pp. 132 - 142 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
华南理工大学食品科学与工程学院,广东广州510640
2022
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1000-565X |
| DOI | 10.12141/j.issn.1000-565X.210145 |
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| Abstract | Q814; 聚对苯二甲酸乙二醇酯(PET)是一种典型的线性热塑性树脂材料,被广泛用于制造纤维、薄膜和饮料瓶.但是,PET为人类带来生活便利的同时也为全球环境造成不利影响,因为其难被降解,造成了大量的白色污染.本研究利用Escherichia.coli BL21(DE3)成功表达重组角质酶LCC,最优表达条件为:诱导剂异丙基-β-D-硫代半乳糖苷(IPTG)浓度0.2 mmol/L,诱导温度16℃,诱导时间20 h.经Ni离子亲和层析纯化后,LCC酶活为320 U/mg.采用超声辅助LCC降解PET,对其超声条件进行优化,温度为65℃,pH=8.0,超声功率为25 W,超声时间为5 h时,LCC动力学参数Km最小,最大反应速度Vmax最高,降解效率是未经处理的1.21倍,产物对苯二甲酸(TPA)质量浓度从0.72 g/L增加到0.87 g/L.通过扫描电镜发现,超声可以加速LCC对PET的降解.采用圆二色光谱和傅里叶变换红外光谱检测LCC二级结构,发现超声可能使LCC的α-螺旋和β-折叠向无规则卷曲转变,由此提高降解速率.这为超声加速降解反应提供了一定的理论依据,也为白色污染的生物降解开创了新思路. |
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| AbstractList | Q814; 聚对苯二甲酸乙二醇酯(PET)是一种典型的线性热塑性树脂材料,被广泛用于制造纤维、薄膜和饮料瓶.但是,PET为人类带来生活便利的同时也为全球环境造成不利影响,因为其难被降解,造成了大量的白色污染.本研究利用Escherichia.coli BL21(DE3)成功表达重组角质酶LCC,最优表达条件为:诱导剂异丙基-β-D-硫代半乳糖苷(IPTG)浓度0.2 mmol/L,诱导温度16℃,诱导时间20 h.经Ni离子亲和层析纯化后,LCC酶活为320 U/mg.采用超声辅助LCC降解PET,对其超声条件进行优化,温度为65℃,pH=8.0,超声功率为25 W,超声时间为5 h时,LCC动力学参数Km最小,最大反应速度Vmax最高,降解效率是未经处理的1.21倍,产物对苯二甲酸(TPA)质量浓度从0.72 g/L增加到0.87 g/L.通过扫描电镜发现,超声可以加速LCC对PET的降解.采用圆二色光谱和傅里叶变换红外光谱检测LCC二级结构,发现超声可能使LCC的α-螺旋和β-折叠向无规则卷曲转变,由此提高降解速率.这为超声加速降解反应提供了一定的理论依据,也为白色污染的生物降解开创了新思路. |
| Author | 宗敏华 娄文勇 吴有权 郭泽望 |
| AuthorAffiliation | 华南理工大学食品科学与工程学院,广东广州510640 |
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| Keywords | 聚对苯二甲酸乙二醇酯;超声;生物降解;角质酶;二级结构 |
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| Title | 角质酶的异源表达和超声辅助下降解PET塑料 |
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