水稻OsMGD2和OsMGD3基因的功能鉴定及其对烟草耐低磷胁迫的影响
Q945.78%S511; [目的]单半乳糖甘油二酯合酶(MGD)是合成单半乳糖甘油二酯(MGDG)的关键酶,在植物响应低磷胁迫过程中起着重要作用.为系统了解水稻OsMGD2和OsMGD3基因在低磷胁迫响应中的作用和功能.[方法]用盆栽试验分析正常和低磷条件下野生型(SR1)、过表达OsMGD2和OsMGD3转基因烟草的生理响应及脂质组分的变化.[结果]无论在正常还是低磷条件下,转基因与野生型烟草的磷含量无显著差异,但转基因烟草的生物量、叶绿素含量和光合能力均显著高于野生型.转基因烟草在低磷胁迫下的磷脂(PL)含量、双半乳糖甘油二酯(DGDG)含量、DGDG与MGDG的比值和半乳糖脂(GL)与...
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| Published in | 西北植物学报 Vol. 44; no. 5; pp. 716 - 728 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
中国科学院水利部水土保持研究所,黄土高原土壤侵蚀与旱地农业国家重点实验室,陕西杨凌 712100
2024
西北农林科技大学资源环境学院,陕西杨凌 712100%商洛学院生物医药与食品工程学院,陕西商洛 726000%西北农林科技大学农学院,陕西杨凌 712100%西北农林科技大学资源环境学院,陕西杨凌 712100 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1000-4025 |
| DOI | 10.7606/j.issn.1000-4025.20230724 |
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| Abstract | Q945.78%S511; [目的]单半乳糖甘油二酯合酶(MGD)是合成单半乳糖甘油二酯(MGDG)的关键酶,在植物响应低磷胁迫过程中起着重要作用.为系统了解水稻OsMGD2和OsMGD3基因在低磷胁迫响应中的作用和功能.[方法]用盆栽试验分析正常和低磷条件下野生型(SR1)、过表达OsMGD2和OsMGD3转基因烟草的生理响应及脂质组分的变化.[结果]无论在正常还是低磷条件下,转基因与野生型烟草的磷含量无显著差异,但转基因烟草的生物量、叶绿素含量和光合能力均显著高于野生型.转基因烟草在低磷胁迫下的磷脂(PL)含量、双半乳糖甘油二酯(DGDG)含量、DGDG与MGDG的比值和半乳糖脂(GL)与PL的比值均显著高于野生型烟草,且OsMGD3转基因烟草的脂质含量和比值均高于OsMGD2转基因烟草.[结论]通过调控水稻OsMGD2/3基因表达,可提高植物低磷下的膜脂重塑能力,进而维持植物在低磷胁迫下较高的光合和生长能力,增加植物的低磷耐受性. |
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| AbstractList | Q945.78%S511; [目的]单半乳糖甘油二酯合酶(MGD)是合成单半乳糖甘油二酯(MGDG)的关键酶,在植物响应低磷胁迫过程中起着重要作用.为系统了解水稻OsMGD2和OsMGD3基因在低磷胁迫响应中的作用和功能.[方法]用盆栽试验分析正常和低磷条件下野生型(SR1)、过表达OsMGD2和OsMGD3转基因烟草的生理响应及脂质组分的变化.[结果]无论在正常还是低磷条件下,转基因与野生型烟草的磷含量无显著差异,但转基因烟草的生物量、叶绿素含量和光合能力均显著高于野生型.转基因烟草在低磷胁迫下的磷脂(PL)含量、双半乳糖甘油二酯(DGDG)含量、DGDG与MGDG的比值和半乳糖脂(GL)与PL的比值均显著高于野生型烟草,且OsMGD3转基因烟草的脂质含量和比值均高于OsMGD2转基因烟草.[结论]通过调控水稻OsMGD2/3基因表达,可提高植物低磷下的膜脂重塑能力,进而维持植物在低磷胁迫下较高的光合和生长能力,增加植物的低磷耐受性. |
| Abstract_FL | [Objective]Monogalactose diglyceride synthase(MGD)is a key enzyme in the synthesis of mo-nogalactose diglyceride(MGDG)and plays an important role in plant response to low phosphorus toler-ance.We aim to understand the role of the OsMGD2 and OsMGD3 genes of rice in response to phosphorus(P)deficiency.[Methods]A pot experiment was conducted to analyze physiological responses and lipid composition changes of the wild-type(SR1)and transgenic tobacco under normal and low P condition.[Results]There was no difference in P content between the transgenic and wild-type tobacco under normal and P deficiency conditions.However,the biomass,chlorophyll content,and photosynthetic electronic transfer of the transgenic tobacco were higher than wild-type.The phospholipid(PL)content,digalactose diglyceride(DGDG)content,DGDG/MGDG ratio,and GL/PL ratio of the transgenic tobacco were higher than those of the wild-type tobacco under low phosphorus deficiency,and the lipid content and ratio of the tobacco expressing OsMGD3 were higher than the tobacco expressing OsMGD2.[Conclusion]Regulation of OsMGD2/3 gene expression in rice can improve lipid remodeling ability and maintain higher photosyn-thetic and growth capacity under phosphorus deficiency,increasing plant tolerance to low phosphorus stress. |
| Author | 王灵龙 席源 张梅娟 王仕稳 李莎莎 殷俐娜 |
| AuthorAffiliation | 西北农林科技大学资源环境学院,陕西杨凌 712100%商洛学院生物医药与食品工程学院,陕西商洛 726000%西北农林科技大学农学院,陕西杨凌 712100%西北农林科技大学资源环境学院,陕西杨凌 712100;中国科学院水利部水土保持研究所,黄土高原土壤侵蚀与旱地农业国家重点实验室,陕西杨凌 712100 |
| AuthorAffiliation_xml | – name: 西北农林科技大学资源环境学院,陕西杨凌 712100%商洛学院生物医药与食品工程学院,陕西商洛 726000%西北农林科技大学农学院,陕西杨凌 712100%西北农林科技大学资源环境学院,陕西杨凌 712100;中国科学院水利部水土保持研究所,黄土高原土壤侵蚀与旱地农业国家重点实验室,陕西杨凌 712100 |
| Author_FL | WANG Linglong WANG Shiwen XI Yuan YIN Li'na LI Shasha ZHANG Meijuan |
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| DocumentTitle_FL | Functional identification of the OsMGD2 and OsMGD3 genes in rice and their influence on phosphorus deficiency in tobacco |
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| Keywords | 磷脂 phosphorus deficiency 双半乳糖甘油二酯 水稻 单半乳糖甘油二酯合酶 膜脂重塑 单半乳糖甘油二酯 MGD rice membrane lipid remodeling 低磷胁迫 转基因烟草 MGDG DGDG transgenic tobacco phospholipid |
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| Publisher | 中国科学院水利部水土保持研究所,黄土高原土壤侵蚀与旱地农业国家重点实验室,陕西杨凌 712100 西北农林科技大学资源环境学院,陕西杨凌 712100%商洛学院生物医药与食品工程学院,陕西商洛 726000%西北农林科技大学农学院,陕西杨凌 712100%西北农林科技大学资源环境学院,陕西杨凌 712100 |
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| Title | 水稻OsMGD2和OsMGD3基因的功能鉴定及其对烟草耐低磷胁迫的影响 |
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