金黄地鼠不同病理状态颊囊黏膜的近红外荧光成像差异分析
R780.2; 目的 探讨分析吲哚菁绿(ICG)近红外荧光(NIF)成像技术对口腔潜在恶性疾患及口腔鳞状细胞癌早期诊断的可行性.方法 利用二甲基苯并蒽丙酮溶液,给予金黄地鼠颊囊黏膜不同时间的局部刺激,诱导建立轻/中度异常增生、重度异常增生、鳞状细胞癌等病理状态的颊黏膜病变模型.采用ICG-NIF技术对病变组织进行荧光信号定量分析,免疫组织化学染色法对不同病理状态黏膜的微血管密度(MVD)、微淋巴管密度(MLVD)进行分析.结果 ICG-NIF荧光定量分析结果显示,实验组中黏膜病变组织的荧光强度均高于正常黏膜,差异具有统计学意义(P＜0.05),且实验组中黏膜病变恶性程度越高,其荧光强度越高.组...
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| Published in | 华西口腔医学杂志 Vol. 42; no. 6; pp. 716 - 722 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
南京大学医学院附属口腔医院,南京市口腔医院,南京大学口腔医学研究所,南京 210008%南京红花社区卫生服务中心口腔科,南京 210008
01.12.2024
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1000-1182 |
| DOI | 10.7518/hxkq.2024.2024150 |
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| Abstract | R780.2; 目的 探讨分析吲哚菁绿(ICG)近红外荧光(NIF)成像技术对口腔潜在恶性疾患及口腔鳞状细胞癌早期诊断的可行性.方法 利用二甲基苯并蒽丙酮溶液,给予金黄地鼠颊囊黏膜不同时间的局部刺激,诱导建立轻/中度异常增生、重度异常增生、鳞状细胞癌等病理状态的颊黏膜病变模型.采用ICG-NIF技术对病变组织进行荧光信号定量分析,免疫组织化学染色法对不同病理状态黏膜的微血管密度(MVD)、微淋巴管密度(MLVD)进行分析.结果 ICG-NIF荧光定量分析结果显示,实验组中黏膜病变组织的荧光强度均高于正常黏膜,差异具有统计学意义(P＜0.05),且实验组中黏膜病变恶性程度越高,其荧光强度越高.组织学分析显示,随着黏膜病变恶性程度增加,MVD增加(P＜0.05),MLVD减小(P＜0.05).结论 金黄地鼠异常增生的颊部黏膜病变与正常黏膜组织间具有ICG-NIF荧光信号差异,荧光定量分析方法具有辅助鉴别的临床应用潜能. |
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| AbstractList | R780.2; 目的 探讨分析吲哚菁绿(ICG)近红外荧光(NIF)成像技术对口腔潜在恶性疾患及口腔鳞状细胞癌早期诊断的可行性.方法 利用二甲基苯并蒽丙酮溶液,给予金黄地鼠颊囊黏膜不同时间的局部刺激,诱导建立轻/中度异常增生、重度异常增生、鳞状细胞癌等病理状态的颊黏膜病变模型.采用ICG-NIF技术对病变组织进行荧光信号定量分析,免疫组织化学染色法对不同病理状态黏膜的微血管密度(MVD)、微淋巴管密度(MLVD)进行分析.结果 ICG-NIF荧光定量分析结果显示,实验组中黏膜病变组织的荧光强度均高于正常黏膜,差异具有统计学意义(P＜0.05),且实验组中黏膜病变恶性程度越高,其荧光强度越高.组织学分析显示,随着黏膜病变恶性程度增加,MVD增加(P＜0.05),MLVD减小(P＜0.05).结论 金黄地鼠异常增生的颊部黏膜病变与正常黏膜组织间具有ICG-NIF荧光信号差异,荧光定量分析方法具有辅助鉴别的临床应用潜能. |
| Abstract_FL | Objective To explore and analyze the feasibility of using indocyanine green(ICG)near-infrared fluores-cence(NIF)imaging technology for the early diagnosis of oral potential malignant disorders and oral squamous cell car-cinoma.Methods 7,12-Dimethylbenz[a]anthracene in acetone solution was used to induce various pathological models of buccal mucosal lesions(mild/moderate dysplasia,severe dysplasia,squamous cell carcinoma)in golden hamster.ICG-NIF was conducted for the quantitative analysis of the fluorescence signal of lesion tissue,and evaluation of the diagnos-tic and discriminative capabilities of the ICG-NIF technology for mucosal lesions in various pathological states.Immuno-histochemical staining was perform to examine the mi-crovessel density(MVD)and microlymphatic vessel den-sity(MLVD)of mucosa in various pathological states and explore the histological reasons underlying the differ-ences in fluorescence signals.Results The results of ICG-NIF fluorescence quantitative analysis reveal the higher fluorescence intensity of mucosal lesions in the experimental group compared with that of the normal mucosa on the control side,with statistical differences(P＜0.05).Moreover,the more severe the malignancy of mucosal lesions in the experimental group,the higher the fluorescence intensity.According to histopathological analysis,the malignant pro-gression of mucosal lesions in golden hamsters was accompanied with an increase in MVD(P＜0.05)and a decrease in MLVD(P＜0.05).Conclusion The abnormal proliferation of mucosal lesions in golden hamsters exhibits a difference in ICG-NIF fluorescence signal compared with normal mucosal tissue.Fluorescence quantitative analysis methods can provide assistance in differentiation and show potential for clinical applications. |
| Author | 单丹妮 张磊 陈盛 那潆予 谢狄亚 王志勇 |
| AuthorAffiliation | 南京大学医学院附属口腔医院,南京市口腔医院,南京大学口腔医学研究所,南京 210008%南京红花社区卫生服务中心口腔科,南京 210008 |
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| Author_FL | Chen Sheng Wang Zhiyong Zhang Lei Xie Diya Shan Danni Na Yingyu |
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| DocumentTitle_FL | Differences in near-infrared fluorescence imaging and histological analysis of cheek mucosa in golden hamsters with different pathological states |
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| Keywords | 吲哚菁绿 oral potentially malignant disorders near-infrared fluorescence imaging indocyanine green 口腔潜在恶性疾患 oral squamous cell carcinoma 口腔鳞状细胞癌 近红外荧光成像 |
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| Publisher | 南京大学医学院附属口腔医院,南京市口腔医院,南京大学口腔医学研究所,南京 210008%南京红花社区卫生服务中心口腔科,南京 210008 |
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| Title | 金黄地鼠不同病理状态颊囊黏膜的近红外荧光成像差异分析 |
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