破骨细胞分化在颞下颌关节骨关节炎发生中的作用

R782.6; 目的 探究破骨细胞分化在颞下颌关节骨关节炎(TMJOA)发生中的作用.方法 构建小鼠TMJOA模型,Micro-CT观察TMJOA发生发展过程中髁突骨质变化,苏木素伊红(HE)染色观察TMJOA关节组织学结构变化,抗酒石酸酸性磷酸酶(TRAP)组织染色观察TMJOA关节组织中破骨细胞的存在情况.收集TMJOA患者滑液,检测其对破骨细胞分化的作用.结果 Micro-CT观察到TMJOA小鼠髁突在第2周和第3周时破坏最明显,形态也发生鸟嘴样改变;HE染色观察到TMJOA小鼠在第2周髁突软骨及软骨下骨结构紊乱;TRAP组织染色显示第2周时关节髁突中破骨细胞数量明显增多;细胞实验结果显...

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Published in华西口腔医学杂志 Vol. 39; no. 4; pp. 398 - 404
Main Authors 肖勉, 胡智慧, 江恒华, 房维, 龙星
Format Journal Article
LanguageChinese
Published 武汉大学口腔医院口腔颌面创伤与颞下颌关节外科,湖北省口腔基础医学重点实验室-省部共建国家重点实验室培育基地,口腔生物医学教育部重点实验室(武汉大学),武汉430000 01.08.2021
Subjects
髁突
破骨细胞
滑液
颞下颌关节骨关节炎
破骨分化
Online AccessGet full text
ISSN1000-1182
DOI10.7518/hxkq.2021.04.004

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Abstract R782.6; 目的 探究破骨细胞分化在颞下颌关节骨关节炎(TMJOA)发生中的作用.方法 构建小鼠TMJOA模型,Micro-CT观察TMJOA发生发展过程中髁突骨质变化,苏木素伊红(HE)染色观察TMJOA关节组织学结构变化,抗酒石酸酸性磷酸酶(TRAP)组织染色观察TMJOA关节组织中破骨细胞的存在情况.收集TMJOA患者滑液,检测其对破骨细胞分化的作用.结果 Micro-CT观察到TMJOA小鼠髁突在第2周和第3周时破坏最明显,形态也发生鸟嘴样改变;HE染色观察到TMJOA小鼠在第2周髁突软骨及软骨下骨结构紊乱;TRAP组织染色显示第2周时关节髁突中破骨细胞数量明显增多;细胞实验结果显示TMJOA患者滑液刺激后的破骨细胞分化数量显著增多,细胞体积变大.结论 TMJOA动物模型及TMJOA患者滑液细胞实验均可诱导破骨细胞分化,表明破骨细胞分化在颞下颌关节骨关节炎的发生中起重要作用.
AbstractList R782.6; 目的 探究破骨细胞分化在颞下颌关节骨关节炎(TMJOA)发生中的作用.方法 构建小鼠TMJOA模型,Micro-CT观察TMJOA发生发展过程中髁突骨质变化,苏木素伊红(HE)染色观察TMJOA关节组织学结构变化,抗酒石酸酸性磷酸酶(TRAP)组织染色观察TMJOA关节组织中破骨细胞的存在情况.收集TMJOA患者滑液,检测其对破骨细胞分化的作用.结果 Micro-CT观察到TMJOA小鼠髁突在第2周和第3周时破坏最明显,形态也发生鸟嘴样改变;HE染色观察到TMJOA小鼠在第2周髁突软骨及软骨下骨结构紊乱;TRAP组织染色显示第2周时关节髁突中破骨细胞数量明显增多;细胞实验结果显示TMJOA患者滑液刺激后的破骨细胞分化数量显著增多,细胞体积变大.结论 TMJOA动物模型及TMJOA患者滑液细胞实验均可诱导破骨细胞分化,表明破骨细胞分化在颞下颌关节骨关节炎的发生中起重要作用.
Author 江恒华
房维
肖勉
龙星
胡智慧
AuthorAffiliation 武汉大学口腔医院口腔颌面创伤与颞下颌关节外科,湖北省口腔基础医学重点实验室-省部共建国家重点实验室培育基地,口腔生物医学教育部重点实验室(武汉大学),武汉430000
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Author_FL Jiang Henghua
Fang Wei
Hu Zhihui
Xiao Mian
Long Xing
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Keywords 髁突
破骨细胞
滑液
颞下颌关节骨关节炎
破骨分化
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