黄腐酚抑制炎症信号缓解大鼠关节炎痛的机制研究
R593.22%R363; 目的:研究黄腐酚(Xn)对大鼠关节炎疼痛的作用及机制.方法:将30只雄性Sprague-Dawley大鼠随机分成3组(每组10只):对照组、关节炎疼痛模型[左后膝关节内注射完全弗氏佐剂(CFA)]组(CFA组)和CFA+Xn(5 mg/kg鞘内注射)组.记录大鼠痛觉行为及运动能力的变化;分子对接分析Xn与糖原合成酶激酶3β(GSK-3β)的作用关系;免疫荧光和Western blot法分析大鼠脊髓组织中GSK-3β的表达及活性、星形胶质细胞激活状态、核因子κB(NF-κB)信号及白细胞介素1β(IL-1β)表达水平;ELISA法检测大鼠血清IL-1β和肿瘤坏死因子α...
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| Published in | 中国病理生理杂志 Vol. 39; no. 6; pp. 1070 - 1076 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
湖北科技学院药学院,湖北 咸宁 437100
01.06.2023
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1000-4718 |
| DOI | 10.3969/j.issn.1000-4718.2023.06.014 |
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| Abstract | R593.22%R363; 目的:研究黄腐酚(Xn)对大鼠关节炎疼痛的作用及机制.方法:将30只雄性Sprague-Dawley大鼠随机分成3组(每组10只):对照组、关节炎疼痛模型[左后膝关节内注射完全弗氏佐剂(CFA)]组(CFA组)和CFA+Xn(5 mg/kg鞘内注射)组.记录大鼠痛觉行为及运动能力的变化;分子对接分析Xn与糖原合成酶激酶3β(GSK-3β)的作用关系;免疫荧光和Western blot法分析大鼠脊髓组织中GSK-3β的表达及活性、星形胶质细胞激活状态、核因子κB(NF-κB)信号及白细胞介素1β(IL-1β)表达水平;ELISA法检测大鼠血清IL-1β和肿瘤坏死因子α(TNF-α)水平.结果:与对照组相比,CFA注射诱导大鼠自发缩足次数增加,机械痛阈值下降,运动能力受损(P＜0.05);脊髓组织中磷酸化GSK-3β、星形胶质细胞标志物胶质细胞原纤维酸性蛋白(GFAP)、NF-κB和IL-1β表达水平均显著升高(P＜0.05);血清中IL-1β和TNF-α水平上调.与CFA组相比,Xn鞘内给药减少大鼠自发缩足次数,上调机械痛阈值,增加转棒停留时间和运动距离(P＜0.05);分子对接分析显示Xn可抑制GSK-3β活性;Xn给药显著降低脊髓组织磷酸化GSK-3β、GFAP、NF-κB和IL-1β蛋白水平(P＜0.05),以及血清中IL-1β和TNF-α水平(P＜0.05).结论:Xn可能通过降低GSK-3β活性抑制炎症信号,从而缓解大鼠关节炎疼痛. |
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| AbstractList | R593.22%R363; 目的:研究黄腐酚(Xn)对大鼠关节炎疼痛的作用及机制.方法:将30只雄性Sprague-Dawley大鼠随机分成3组(每组10只):对照组、关节炎疼痛模型[左后膝关节内注射完全弗氏佐剂(CFA)]组(CFA组)和CFA+Xn(5 mg/kg鞘内注射)组.记录大鼠痛觉行为及运动能力的变化;分子对接分析Xn与糖原合成酶激酶3β(GSK-3β)的作用关系;免疫荧光和Western blot法分析大鼠脊髓组织中GSK-3β的表达及活性、星形胶质细胞激活状态、核因子κB(NF-κB)信号及白细胞介素1β(IL-1β)表达水平;ELISA法检测大鼠血清IL-1β和肿瘤坏死因子α(TNF-α)水平.结果:与对照组相比,CFA注射诱导大鼠自发缩足次数增加,机械痛阈值下降,运动能力受损(P＜0.05);脊髓组织中磷酸化GSK-3β、星形胶质细胞标志物胶质细胞原纤维酸性蛋白(GFAP)、NF-κB和IL-1β表达水平均显著升高(P＜0.05);血清中IL-1β和TNF-α水平上调.与CFA组相比,Xn鞘内给药减少大鼠自发缩足次数,上调机械痛阈值,增加转棒停留时间和运动距离(P＜0.05);分子对接分析显示Xn可抑制GSK-3β活性;Xn给药显著降低脊髓组织磷酸化GSK-3β、GFAP、NF-κB和IL-1β蛋白水平(P＜0.05),以及血清中IL-1β和TNF-α水平(P＜0.05).结论:Xn可能通过降低GSK-3β活性抑制炎症信号,从而缓解大鼠关节炎疼痛. |
| Author | 谢敏 王钦 王柏军 |
| AuthorAffiliation | 湖北科技学院药学院,湖北 咸宁 437100 |
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| Author_FL | WANG Qin XIE Min WANG Bojun |
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| Author_xml | – sequence: 1 fullname: 王钦 – sequence: 2 fullname: 谢敏 – sequence: 3 fullname: 王柏军 |
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| Keywords | 糖原合成酶激酶3β 关节炎痛 大鼠 黄腐酚 炎症 |
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