舒眠胶囊的镇静催眠作用及其机制

R285.5; 目的 研究舒眠胶囊中枢镇静催眠作用及其机制.方法 分别设空白对照组、阳性药地西泮组及舒眠胶囊低、中、高剂量组.雄性昆明小鼠连续灌胃给药,分别进行小鼠自主活动实验、戊巴比妥钠阈上和阈下剂量睡眠实验;采用酶联免疫吸附法(ELISA)及比色法测定小鼠脑组织γ-氨基丁酸(GABA)、谷氨酸(Glu)含量.雄性SD大鼠腹腔注射350mg/(kg·d)对氯苯丙氨酸(PCPA)制备大鼠失眠模型,造模后按实验分组分别连续灌胃给药,观察大鼠一般状况和体质量变化,旷场实验测试大鼠运动总路程和站立次数;Western blotting和免疫组化检测大鼠海马5-HT1A受体(5-HT1A R)蛋白表达...

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Published in西安交通大学学报（医学版） Vol. 42; no. 1; pp. 168 - 174
Main Authors 梁凡凡, 张鑫, 蒋茜茜, 张凯娜, AL-MAAMARI Ahmed, 王红英, 赵正杭
Format Journal Article
LanguageChinese
Published 西安交通大学医学部基础医学院药理学系,陕西西安 710061 05.01.2021
Subjects
5-羟色胺1A受体
镇静催眠
谷氨酸
舒眠胶囊
γ-氨基丁酸
Online AccessGet full text
ISSN1671-8259
DOI10.7652/jdyxb202101031

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Abstract R285.5; 目的 研究舒眠胶囊中枢镇静催眠作用及其机制.方法 分别设空白对照组、阳性药地西泮组及舒眠胶囊低、中、高剂量组.雄性昆明小鼠连续灌胃给药,分别进行小鼠自主活动实验、戊巴比妥钠阈上和阈下剂量睡眠实验;采用酶联免疫吸附法(ELISA)及比色法测定小鼠脑组织γ-氨基丁酸(GABA)、谷氨酸(Glu)含量.雄性SD大鼠腹腔注射350mg/(kg·d)对氯苯丙氨酸(PCPA)制备大鼠失眠模型,造模后按实验分组分别连续灌胃给药,观察大鼠一般状况和体质量变化,旷场实验测试大鼠运动总路程和站立次数;Western blotting和免疫组化检测大鼠海马5-HT1A受体(5-HT1A R)蛋白表达.结果 与空白对照组相比,连续给药14d,1.6、3.2、6.4mg/(kg·d)舒眠胶囊各剂量组均可明显减少小鼠的活动次数及站立次数(P<0.01),高剂量组明显延长阈上剂量戊巴比妥钠致小鼠睡眠持续时间(P<0.01);舒眠胶囊各剂量组可剂量依赖性升高小鼠脑组织GABA水平(P<0.05),高、中剂量组均明显降低小鼠海马Glu水平(P<0.05),高剂量组明显降低皮质Glu水平(P<0.05).失眠模型大鼠出现活动萎靡不振,昼夜活动节律消失,毛发变硬,粗糙无光泽,掉毛现象严重,体质量减轻等现象;连续给药7d,舒眠胶囊各剂量组大鼠精神状况得到改善,毛发相对润泽,掉毛现象改善;与空白对照组比较,腹腔注射PCPA后,大鼠体质量增量显著降低(P<0.01);与模型组比较,舒眠胶囊各剂量组大鼠体质量增量明显增加(P<0.01).模型组大鼠在旷场实验中运动距离延长,海马5-HT1AR蛋白表达水平下降;而地西泮组和舒眠胶囊各剂量组运动距离显著减少(P<0.01),且地西泮及舒眠胶囊高、中剂量组海马5-HT1AR蛋白表达明显上调(P<0.05).结论 舒眠胶囊有镇静、催眠、改善实验性动物失眠等作用,其机制可能与升高大脑组织GABA水平,降低Glu含量,上调海马5-HT1AR蛋白表达有关.
AbstractList R285.5; 目的 研究舒眠胶囊中枢镇静催眠作用及其机制.方法 分别设空白对照组、阳性药地西泮组及舒眠胶囊低、中、高剂量组.雄性昆明小鼠连续灌胃给药,分别进行小鼠自主活动实验、戊巴比妥钠阈上和阈下剂量睡眠实验;采用酶联免疫吸附法(ELISA)及比色法测定小鼠脑组织γ-氨基丁酸(GABA)、谷氨酸(Glu)含量.雄性SD大鼠腹腔注射350mg/(kg·d)对氯苯丙氨酸(PCPA)制备大鼠失眠模型,造模后按实验分组分别连续灌胃给药,观察大鼠一般状况和体质量变化,旷场实验测试大鼠运动总路程和站立次数;Western blotting和免疫组化检测大鼠海马5-HT1A受体(5-HT1A R)蛋白表达.结果 与空白对照组相比,连续给药14d,1.6、3.2、6.4mg/(kg·d)舒眠胶囊各剂量组均可明显减少小鼠的活动次数及站立次数(P<0.01),高剂量组明显延长阈上剂量戊巴比妥钠致小鼠睡眠持续时间(P<0.01);舒眠胶囊各剂量组可剂量依赖性升高小鼠脑组织GABA水平(P<0.05),高、中剂量组均明显降低小鼠海马Glu水平(P<0.05),高剂量组明显降低皮质Glu水平(P<0.05).失眠模型大鼠出现活动萎靡不振,昼夜活动节律消失,毛发变硬,粗糙无光泽,掉毛现象严重,体质量减轻等现象;连续给药7d,舒眠胶囊各剂量组大鼠精神状况得到改善,毛发相对润泽,掉毛现象改善;与空白对照组比较,腹腔注射PCPA后,大鼠体质量增量显著降低(P<0.01);与模型组比较,舒眠胶囊各剂量组大鼠体质量增量明显增加(P<0.01).模型组大鼠在旷场实验中运动距离延长,海马5-HT1AR蛋白表达水平下降;而地西泮组和舒眠胶囊各剂量组运动距离显著减少(P<0.01),且地西泮及舒眠胶囊高、中剂量组海马5-HT1AR蛋白表达明显上调(P<0.05).结论 舒眠胶囊有镇静、催眠、改善实验性动物失眠等作用,其机制可能与升高大脑组织GABA水平,降低Glu含量,上调海马5-HT1AR蛋白表达有关.
Author 张凯娜
梁凡凡
AL-MAAMARI Ahmed
张鑫
蒋茜茜
王红英
赵正杭
AuthorAffiliation 西安交通大学医学部基础医学院药理学系,陕西西安 710061
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Author_FL ZHAO Zhenghang
Al-MAAMARI Ahmed
ZHANG Xin
JIANG Xixi
WANG Hongying
ZHANG Kaina
LIANG Fanfan
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Keywords 5-羟色胺1A受体
镇静催眠
谷氨酸
舒眠胶囊
γ-氨基丁酸
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Snippet R285.5; 目的 研究舒眠胶囊中枢镇静催眠作用及其机制.方法 分别设空白对照组、阳性药地西泮组及舒眠胶囊低、中、高剂量组.雄性昆明小鼠连续灌胃给药,分别进行小鼠自主活动实...
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Title 舒眠胶囊的镇静催眠作用及其机制
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