CD133+干细胞在胰腺癌放疗耐受中的作用

R735.9; 目的 研究肿瘤干细胞在胰腺癌放疗耐受中的作用.方法 应用磁珠分选法分选CD133+胰腺癌干细胞,首先用MTT法和流式细胞仪观察放射线干预后胰腺癌不同细胞亚群的增殖活性及细胞凋亡;流式细胞仪、Western blot和qRT-PCR检测放射线干预后胰腺癌细胞CD133的表达变化;流式细胞仪观察不同细胞亚群的细胞周期分布.结果 用放射线干预后,CD133+干细胞与未分选细胞及CD133-细胞相比,存活率最高、凋亡率最低,且胰腺癌细胞中CD133+干细胞比例上升,CD133蛋白和RNA表达均上调;CD133+干细胞与未分选细胞和CD133-细胞相比,G0/G1期比例最高,且差异具有统...

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Published in西安交通大学学报（医学版） Vol. 41; no. 2; pp. 216 - 220
Main Authors 王敏聪, 王中卫, 黄蓝萱, 韩丽丽, 谢非
Format Journal Article
LanguageChinese
Published 西安交通大学第二附属医院肿瘤放疗科,陕西西安,710004%西安交通大学第二附属医院肿瘤内科,陕西西安,710004%西安交通大学附属西安市中心医院,陕西西安,710003 05.03.2020
Subjects
胰腺癌
放疗耐受
肿瘤干细胞
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ISSN1671-8259
DOI10.7652/jdyxb202002012

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Abstract R735.9; 目的 研究肿瘤干细胞在胰腺癌放疗耐受中的作用.方法 应用磁珠分选法分选CD133+胰腺癌干细胞,首先用MTT法和流式细胞仪观察放射线干预后胰腺癌不同细胞亚群的增殖活性及细胞凋亡;流式细胞仪、Western blot和qRT-PCR检测放射线干预后胰腺癌细胞CD133的表达变化;流式细胞仪观察不同细胞亚群的细胞周期分布.结果 用放射线干预后,CD133+干细胞与未分选细胞及CD133-细胞相比,存活率最高、凋亡率最低,且胰腺癌细胞中CD133+干细胞比例上升,CD133蛋白和RNA表达均上调;CD133+干细胞与未分选细胞和CD133-细胞相比,G0/G1期比例最高,且差异具有统计学意义.结论 CD133+胰腺癌干细胞是导致胰腺癌细胞放疗耐受的重要原因之一,而G0/G1期比例高可能是其机制之一.
AbstractList R735.9; 目的 研究肿瘤干细胞在胰腺癌放疗耐受中的作用.方法 应用磁珠分选法分选CD133+胰腺癌干细胞,首先用MTT法和流式细胞仪观察放射线干预后胰腺癌不同细胞亚群的增殖活性及细胞凋亡;流式细胞仪、Western blot和qRT-PCR检测放射线干预后胰腺癌细胞CD133的表达变化;流式细胞仪观察不同细胞亚群的细胞周期分布.结果 用放射线干预后,CD133+干细胞与未分选细胞及CD133-细胞相比,存活率最高、凋亡率最低,且胰腺癌细胞中CD133+干细胞比例上升,CD133蛋白和RNA表达均上调;CD133+干细胞与未分选细胞和CD133-细胞相比,G0/G1期比例最高,且差异具有统计学意义.结论 CD133+胰腺癌干细胞是导致胰腺癌细胞放疗耐受的重要原因之一,而G0/G1期比例高可能是其机制之一.
Author 王敏聪
王中卫
黄蓝萱
韩丽丽
谢非
AuthorAffiliation 西安交通大学第二附属医院肿瘤放疗科,陕西西安,710004%西安交通大学第二附属医院肿瘤内科,陕西西安,710004%西安交通大学附属西安市中心医院,陕西西安,710003
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WANG Zhongwei
XIE Fei
HUANG Lanxuan
HAN Lili
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Keywords 胰腺癌
放疗耐受
肿瘤干细胞
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Snippet R735.9; 目的 研究肿瘤干细胞在胰腺癌放疗耐受中的作用.方法 应用磁珠分选法分选CD133+胰腺癌干细胞,首先用MTT法和流式细胞仪观察放射线干预后胰腺癌不同细胞亚群的增殖活...
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Title CD133+干细胞在胰腺癌放疗耐受中的作用
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