低温等离子活化水对猕猴桃溃疡病菌抗菌活性及机制
S126; 为了探究低温等离子体活化水(cold plasma activated water,PAW)对丁香假单胞杆菌猕猴桃致病变种(Pseudomonas syringae pv.actinidiae,PSA)的抗菌活性和潜在机制.该研究通过介质阻挡放电(dielectric barrier discharge,DBD)低温等离子体发生装置制备不同激活时间的PAW,考察放电时间与杀菌效果之间的关系.此外,通过评估PSA形态特征,细胞粒径、DNA、细胞膜损伤情况和胞内活性氧积累(reactive oxygen species,ROS)情况探究PAW对PSA的杀菌机制.结果表明:PAW对PSA...
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| Published in | 农业工程学报 Vol. 40; no. 8; pp. 264 - 272 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
西北农林科技大学食品科学与工程学院,杨凌 712100%陕西省农村科技发展中心,西安 710000
01.04.2024
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1002-6819 |
| DOI | 10.11975/j.issn.1002-6819.202312181 |
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| Abstract | S126; 为了探究低温等离子体活化水(cold plasma activated water,PAW)对丁香假单胞杆菌猕猴桃致病变种(Pseudomonas syringae pv.actinidiae,PSA)的抗菌活性和潜在机制.该研究通过介质阻挡放电(dielectric barrier discharge,DBD)低温等离子体发生装置制备不同激活时间的PAW,考察放电时间与杀菌效果之间的关系.此外,通过评估PSA形态特征,细胞粒径、DNA、细胞膜损伤情况和胞内活性氧积累(reactive oxygen species,ROS)情况探究PAW对PSA的杀菌机制.结果表明:PAW对PSA的杀灭效果与PAW激活时间呈依赖性,与对照相比PAW处理120 s后,PSA显著(P＜0.05)减少了4.38 lg CFU/mL.扫描电子显微镜(field emission scanning electron microscope,FESEM)结果清楚地表明,由于PAW处理,PSA细胞发生了明显的质壁分离.荧光染色结果显示,PSA细胞DNA、膜渗透屏障被破坏程度、内容物泄漏量和ROS积累量与PAW激活时间表现为正相关关系.因此,推测PAW由于自身酸性、较高的氧化还原电位(oxidation-reduction potential,ORP),以及水性活性物质导致细胞的氧化损伤,而且这可能是杀灭PSA的主要原因.研究结果可为PAW控制猕猴桃细菌性溃疡病提供参考. |
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| AbstractList | S126; 为了探究低温等离子体活化水(cold plasma activated water,PAW)对丁香假单胞杆菌猕猴桃致病变种(Pseudomonas syringae pv.actinidiae,PSA)的抗菌活性和潜在机制.该研究通过介质阻挡放电(dielectric barrier discharge,DBD)低温等离子体发生装置制备不同激活时间的PAW,考察放电时间与杀菌效果之间的关系.此外,通过评估PSA形态特征,细胞粒径、DNA、细胞膜损伤情况和胞内活性氧积累(reactive oxygen species,ROS)情况探究PAW对PSA的杀菌机制.结果表明:PAW对PSA的杀灭效果与PAW激活时间呈依赖性,与对照相比PAW处理120 s后,PSA显著(P＜0.05)减少了4.38 lg CFU/mL.扫描电子显微镜(field emission scanning electron microscope,FESEM)结果清楚地表明,由于PAW处理,PSA细胞发生了明显的质壁分离.荧光染色结果显示,PSA细胞DNA、膜渗透屏障被破坏程度、内容物泄漏量和ROS积累量与PAW激活时间表现为正相关关系.因此,推测PAW由于自身酸性、较高的氧化还原电位(oxidation-reduction potential,ORP),以及水性活性物质导致细胞的氧化损伤,而且这可能是杀灭PSA的主要原因.研究结果可为PAW控制猕猴桃细菌性溃疡病提供参考. |
| Abstract_FL | Kiwifruit bacterial canker has been one of the most devastating diseases in global agriculture,due to its highly destructive nature and persistent presence.Pseudomonas syringae pv.actinidiae(PSA)has been identified as the causative agent responsible for the kiwifruit bacterial canker.Among them,cold Plasma(CP)can be expected to partially or fully ionize the agent at ambient temperature.The sources of CP can be the dielectric barrier discharge(DBD),gliding arc discharge,corona discharge plasma jet,and microwave/radio frequency plasma.In interaction with water,CP can be used to induce the plasma-activated water(PAW)at the elevated levels of reactive oxygen species(ROS)and reactive nitrogen species(RNS).This transformation of CP into PAW can be potential for agriculture and biomedicine,due to the significant antimicrobial properties of ROS and RNS content.A systematic exploration was conducted to explore the antimicrobial efficacy and potential mechanism of PAW against PSA,particularly the relationship between discharge time and the bactericidal effect of PAW.Different PAW samples were prepared with the activation times of 0,30,60,90,and 120 s in a generator equipped with DBD.The discharge parameters were given:discharge power 40 W,(input voltage 40 V,current(1.0±0.1)A),frequency 200 Hz,and the air medium with an air gap of 3 mm.The bactericidal mechanism of PAW on PSA was investigated to determine the pH,oxidation reduction potential(ORP),conductivity,and concentrations of three oxidizing agents,including hydrogen peroxide(H2O2),ozone,and nitrite ions in the samples of PAW.PSA morphology was examined to assess the DNA integrity,damage to the cell membrane,intracellular ROS accumulation,and leakage of contents using particle size analysis and fluorescent staining.The inactivation mechanism of PSA was investigated by PAW.There was a significant correlation between the activation time and the bactericidal efficacy with a significant(P＜0.05)reduction of 4.38 lg CFU/mL after 120 s of PAW treatment,compared with the control.The acidification of sterile deionized water was attributed to H2O2,ozone,and nitrite ions during plasma discharge.The conductivity,ORP,and concentration of the three substances were then determined in the PAW.There was a significant increase(P＜0.05)with increasing discharge time.In addition,the low pH environment of the PAW was used to maintain its bactericidal efficacy.There was a positive correlation among the DNA damage,membrane permeability barrier disruption,leakage of intracellular substances,and the accumulation of ROS with the PAW activation time.The lethal impact of PAW on PSA was attributed to cellular oxidative damage.The inherent acidic pH was induced to elevate the redox potential in the presence of aqueous active substances,such as ozone,H2O2,and nitrite ions in the PAW.The antimicrobial mechanisms of PAW against PSA greatly contributed to the potential applications and prevention of agricultural disease. |
| Author | 刘芊辰 雷玉山 徐明 史文青 江昊 |
| AuthorAffiliation | 西北农林科技大学食品科学与工程学院,杨凌 712100%陕西省农村科技发展中心,西安 710000 |
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| Author_FL | SHI Wenqing JIANG Hao LEI Yushan LIU Qianchen XU Ming |
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| DocumentTitle_FL | Investigation of the antimicrobial activity and mechanism of cold plasma activated water against kiwifruit canker pathogens |
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| Keywords | 杀菌机制 介质阻挡放电 cells dielectric barrier discharge(DBD) 丁香假单胞杆菌猕猴桃致病变种 细胞 oxidative damage 氧化损伤 cold plasma activated water(PAW) sterilization mechanism 低温等离子体活化水 Pseudomonas syringae pv.actinidiae(PSA) |
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| Publisher | 西北农林科技大学食品科学与工程学院,杨凌 712100%陕西省农村科技发展中心,西安 710000 |
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| Title | 低温等离子活化水对猕猴桃溃疡病菌抗菌活性及机制 |
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