桦褐孔菌多糖的分离纯化及其抗氧化活性测定

TS201.2; 以桦褐孔菌为原料,测定其化学组分,并采用水提醇沉法提取桦褐孔菌粗多糖(IOP).将获取的桦褐孔菌粗多糖进行脱蛋白、脱色素的初步纯化,然后利用DEAE-52纤维素层析柱及Sephadex-100凝胶柱对多糖提取液进行分离纯化,通过抗氧化活性筛选出活性较高的多糖组分,并通过高效液相色谱分析多糖组分的纯度.实验结果表明,桦褐孔菌子实体的化学成分组成丰富,其中多糖13.10%±0.31%、还原糖4.21%±0.40%、蛋白质2.70%±0.71%、灰分9.60%±0.31%.通过对桦褐孔菌粗多糖脱蛋白、脱色素的试验方法进行筛选,得到聚酰胺层析法为最佳的脱除方法.蛋白质的脱除率为93....

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Published in食品工业科技 Vol. 42; no. 11; pp. 192 - 197
Main Authors 李欣欣, 李文香
Format Journal Article
LanguageChinese
Published 青岛农业大学食品科学与工程学院,山东青岛266109 01.06.2021
Subjects
Online AccessGet full text
ISSN1002-0306
DOI10.13386/j.issn1002-0306.2020040211

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Abstract TS201.2; 以桦褐孔菌为原料,测定其化学组分,并采用水提醇沉法提取桦褐孔菌粗多糖(IOP).将获取的桦褐孔菌粗多糖进行脱蛋白、脱色素的初步纯化,然后利用DEAE-52纤维素层析柱及Sephadex-100凝胶柱对多糖提取液进行分离纯化,通过抗氧化活性筛选出活性较高的多糖组分,并通过高效液相色谱分析多糖组分的纯度.实验结果表明,桦褐孔菌子实体的化学成分组成丰富,其中多糖13.10%±0.31%、还原糖4.21%±0.40%、蛋白质2.70%±0.71%、灰分9.60%±0.31%.通过对桦褐孔菌粗多糖脱蛋白、脱色素的试验方法进行筛选,得到聚酰胺层析法为最佳的脱除方法.蛋白质的脱除率为93.1%、脱色率为75.8%,多糖的保留率为90.3%.IOP经过DEAE-52纤维素柱层析后得到四个单糖组分:IOP-1、IOP-2、IOP-3、IOP-4.对四个多糖组分进行抗氧化实验发现,IOP-2对DPPH自由基的清除率最高,清除率为81.9%.IOP-2经过Sephadex-100层析柱后获得两个多糖组分:IOP-2a、IOP-2b.对比其抗氧化活性,筛选出活性较高的多糖组分为IOP-2a.采用高效液相色谱法鉴定多糖组分IOP-2a,只检查出一个对称峰,说明IOP-2a为均一性多糖.
AbstractList TS201.2; 以桦褐孔菌为原料,测定其化学组分,并采用水提醇沉法提取桦褐孔菌粗多糖(IOP).将获取的桦褐孔菌粗多糖进行脱蛋白、脱色素的初步纯化,然后利用DEAE-52纤维素层析柱及Sephadex-100凝胶柱对多糖提取液进行分离纯化,通过抗氧化活性筛选出活性较高的多糖组分,并通过高效液相色谱分析多糖组分的纯度.实验结果表明,桦褐孔菌子实体的化学成分组成丰富,其中多糖13.10%±0.31%、还原糖4.21%±0.40%、蛋白质2.70%±0.71%、灰分9.60%±0.31%.通过对桦褐孔菌粗多糖脱蛋白、脱色素的试验方法进行筛选,得到聚酰胺层析法为最佳的脱除方法.蛋白质的脱除率为93.1%、脱色率为75.8%,多糖的保留率为90.3%.IOP经过DEAE-52纤维素柱层析后得到四个单糖组分:IOP-1、IOP-2、IOP-3、IOP-4.对四个多糖组分进行抗氧化实验发现,IOP-2对DPPH自由基的清除率最高,清除率为81.9%.IOP-2经过Sephadex-100层析柱后获得两个多糖组分:IOP-2a、IOP-2b.对比其抗氧化活性,筛选出活性较高的多糖组分为IOP-2a.采用高效液相色谱法鉴定多糖组分IOP-2a,只检查出一个对称峰,说明IOP-2a为均一性多糖.
Author 李文香
李欣欣
AuthorAffiliation 青岛农业大学食品科学与工程学院,山东青岛266109
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Keywords 桦褐孔菌
抗氧化
高效液相色谱
分离纯化
多糖
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Snippet TS201.2; 以桦褐孔菌为原料,测定其化学组分,并采用水提醇沉法提取桦褐孔菌粗多糖(IOP).将获取的桦褐孔菌粗多糖进行脱蛋白、脱色素的初步纯化,然后利用DEAE-52纤维素层析柱...
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Title 桦褐孔菌多糖的分离纯化及其抗氧化活性测定
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