响应面法优化蛋壳豆渣发酵工艺生产高钙饲料

TQ920; 大量废弃的蛋壳和湿豆渣是良好的钙和氮素资源,为探索一条解决两类农业废弃物合理利用的新途径,本实验选用枯草芽孢杆菌、植物乳杆菌和粪肠球菌固体生料发酵鸡蛋壳、豆渣混合物.以乳酸钙含量为指标,利用单因素实验研究了豆渣蛋壳比例、固水比、葡萄糖添加量、接种量、混合菌种比例、发酵温度和发酵时间对发酵产乳酸钙的影响,并利用响应面试验优化发酵产乳酸钙的工艺条件.实验结果表明:在豆渣蛋壳比例90:10、枯草芽孢杆菌:植物乳杆菌:粪肠球菌为1:1:1(体积比)、固水比1:2.5、葡萄糖添加量15.6％、接菌量14.7％、发酵温度37℃、发酵6 d的条件下,蛋壳豆渣混合物中的乳酸钙含量达到13.58％...

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Published in食品工业科技 Vol. 44; no. 9; pp. 200 - 206
Main Authors 陈劭舒, 董丽婷, 夏凤腾, 刘玉洁, 罗灿, 李怡成, 王征
Format Journal Article
LanguageChinese
Published 湖南农业大学生物科学技术学院,湖南长沙 410128%湖南畅想农业科技有限公司,湖南张家界 427000 01.05.2023
Subjects
固体发酵
鸡蛋壳
豆渣
乳酸钙
高钙饲料
Online AccessGet full text
ISSN1002-0306
DOI10.13386/j.issn1002-0306.2022070321

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Abstract TQ920; 大量废弃的蛋壳和湿豆渣是良好的钙和氮素资源,为探索一条解决两类农业废弃物合理利用的新途径,本实验选用枯草芽孢杆菌、植物乳杆菌和粪肠球菌固体生料发酵鸡蛋壳、豆渣混合物.以乳酸钙含量为指标,利用单因素实验研究了豆渣蛋壳比例、固水比、葡萄糖添加量、接种量、混合菌种比例、发酵温度和发酵时间对发酵产乳酸钙的影响,并利用响应面试验优化发酵产乳酸钙的工艺条件.实验结果表明:在豆渣蛋壳比例90:10、枯草芽孢杆菌:植物乳杆菌:粪肠球菌为1:1:1(体积比)、固水比1:2.5、葡萄糖添加量15.6％、接菌量14.7％、发酵温度37℃、发酵6 d的条件下,蛋壳豆渣混合物中的乳酸钙含量达到13.58％.与发酵前相比,发酵后豆渣饲料中的游离氨基酸、乳酸钙含量显著提高(P<0.05),pH、粗脂肪、可溶性蛋白显著降低(P<0.05).综上所述,利用益生菌发酵蛋壳与豆渣,可以制备一种高钙饲料.
AbstractList TQ920; 大量废弃的蛋壳和湿豆渣是良好的钙和氮素资源,为探索一条解决两类农业废弃物合理利用的新途径,本实验选用枯草芽孢杆菌、植物乳杆菌和粪肠球菌固体生料发酵鸡蛋壳、豆渣混合物.以乳酸钙含量为指标,利用单因素实验研究了豆渣蛋壳比例、固水比、葡萄糖添加量、接种量、混合菌种比例、发酵温度和发酵时间对发酵产乳酸钙的影响,并利用响应面试验优化发酵产乳酸钙的工艺条件.实验结果表明:在豆渣蛋壳比例90:10、枯草芽孢杆菌:植物乳杆菌:粪肠球菌为1:1:1(体积比)、固水比1:2.5、葡萄糖添加量15.6％、接菌量14.7％、发酵温度37℃、发酵6 d的条件下,蛋壳豆渣混合物中的乳酸钙含量达到13.58％.与发酵前相比,发酵后豆渣饲料中的游离氨基酸、乳酸钙含量显著提高(P<0.05),pH、粗脂肪、可溶性蛋白显著降低(P<0.05).综上所述,利用益生菌发酵蛋壳与豆渣,可以制备一种高钙饲料.
Author 夏凤腾
刘玉洁
李怡成
陈劭舒
董丽婷
罗灿
王征
AuthorAffiliation 湖南农业大学生物科学技术学院,湖南长沙 410128%湖南畅想农业科技有限公司,湖南张家界 427000
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Author_FL LIU Yujie
LI Yicheng
CHEN Shaoshu
LUO Can
WANG Zheng
DONG Liting
XIA Fengteng
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Keywords 固体发酵
鸡蛋壳
豆渣
乳酸钙
高钙饲料
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Title 响应面法优化蛋壳豆渣发酵工艺生产高钙饲料
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