双氢青蒿素对MRL/lpr狼疮鼠的治疗作用及其对滤泡辅助性T细胞的调控作用
R593.24; 目的 探讨双氢青蒿素对MRL/lpr狼疮鼠的治疗作用及其对滤泡辅助性T细胞(T follicular helper cells,TFH)的调控作用.方法 将12只雌性狼疮鼠(12周龄)随机分为双氢青蒿素治疗组和对照组.治疗组双氢青蒿素(150 mg/kg)灌胃给药,对照组给予PBS溶液,均1次/d,持续给药4周至16周龄时,考马斯亮蓝法检测24 h尿蛋白,其后处死狼疮鼠.取肾脏行苏木精-伊红染色,采用肾脏病理评分评估肾脏损伤情况;收集血清,ELISA检测抗ds-DNA抗体及ANA抗体含量;取脾脏称重并分离单个核细胞,流式细胞仪检测滤泡辅助性T细胞百分比.体外实验中,免疫磁珠分...
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| Published in | 中国临床医学 Vol. 29; no. 3; pp. 372 - 376 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
复旦大学附属中山医院皮肤科,上海 200032
01.06.2022
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1008-6358 |
| DOI | 10.12025/j.issn.1008-6358.2022.20220257 |
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| Abstract | R593.24; 目的 探讨双氢青蒿素对MRL/lpr狼疮鼠的治疗作用及其对滤泡辅助性T细胞(T follicular helper cells,TFH)的调控作用.方法 将12只雌性狼疮鼠(12周龄)随机分为双氢青蒿素治疗组和对照组.治疗组双氢青蒿素(150 mg/kg)灌胃给药,对照组给予PBS溶液,均1次/d,持续给药4周至16周龄时,考马斯亮蓝法检测24 h尿蛋白,其后处死狼疮鼠.取肾脏行苏木精-伊红染色,采用肾脏病理评分评估肾脏损伤情况;收集血清,ELISA检测抗ds-DNA抗体及ANA抗体含量;取脾脏称重并分离单个核细胞,流式细胞仪检测滤泡辅助性T细胞百分比.体外实验中,免疫磁珠分选MRL/lpr狼疮鼠中初始CD4+T细胞,根据双氢青蒿素干预的不同浓度分为4组(0、0.1、1.0、10μmol/L),每组加入10 ng/mL IL-21、20 ng/mL IL-6和T细胞活化/扩增试剂盒中的试剂诱导分化5 d,流式细胞仪检测TFH细胞比例;收集TFH细胞培养上清,ELISA检测TFH细胞分泌的IL-21含量.结果 双氢青蒿素治疗组尿蛋白(1536±150.1)μg/24 h显著小于对照组(2548±93.30)μg/24 h(P<0.01),治疗组肾脏病理评分更低(2.410±0.535)vs(5.713±0.662),P<0.01.治疗组抗ds-DNA抗体低于对照组[(345.3±29.00)vs(477.8±20.07)IU/mL,t=7.514,P<0.01];治疗组ANA低于对照组[(514.5±19.64)vs(726.3±16.27)IU/mL,t=8.302,P<0.01].治疗组TFH细胞比例(12.40％±2.726％)低于对照组(36.33％±2.750％),P<0.01.体外双氢青蒿素可抑制TFH细胞分化,0、0.1、1.0、10μmol/L双氢青蒿素剂量依赖性抑制TFH细胞百分比(分别为29.43％±1.94％、21.07％±1.19％、11.27％±1.40％、6.28％±1.02％;P<0.01),且抑制细胞因子IL-21的分泌[分别为(87.07±5.49)、(63.63±3.70)、(47.67±4.02)、(34.37±5.10)pg/mL,P<0.01].结论 双氢青蒿素对MRL/lpr狼疮鼠具有治疗作用,可有效改善狼疮肾炎、降低抗体滴度,其机制可能与抑制TFH细胞 |
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| AbstractList | R593.24; 目的 探讨双氢青蒿素对MRL/lpr狼疮鼠的治疗作用及其对滤泡辅助性T细胞(T follicular helper cells,TFH)的调控作用.方法 将12只雌性狼疮鼠(12周龄)随机分为双氢青蒿素治疗组和对照组.治疗组双氢青蒿素(150 mg/kg)灌胃给药,对照组给予PBS溶液,均1次/d,持续给药4周至16周龄时,考马斯亮蓝法检测24 h尿蛋白,其后处死狼疮鼠.取肾脏行苏木精-伊红染色,采用肾脏病理评分评估肾脏损伤情况;收集血清,ELISA检测抗ds-DNA抗体及ANA抗体含量;取脾脏称重并分离单个核细胞,流式细胞仪检测滤泡辅助性T细胞百分比.体外实验中,免疫磁珠分选MRL/lpr狼疮鼠中初始CD4+T细胞,根据双氢青蒿素干预的不同浓度分为4组(0、0.1、1.0、10μmol/L),每组加入10 ng/mL IL-21、20 ng/mL IL-6和T细胞活化/扩增试剂盒中的试剂诱导分化5 d,流式细胞仪检测TFH细胞比例;收集TFH细胞培养上清,ELISA检测TFH细胞分泌的IL-21含量.结果 双氢青蒿素治疗组尿蛋白(1536±150.1)μg/24 h显著小于对照组(2548±93.30)μg/24 h(P<0.01),治疗组肾脏病理评分更低(2.410±0.535)vs(5.713±0.662),P<0.01.治疗组抗ds-DNA抗体低于对照组[(345.3±29.00)vs(477.8±20.07)IU/mL,t=7.514,P<0.01];治疗组ANA低于对照组[(514.5±19.64)vs(726.3±16.27)IU/mL,t=8.302,P<0.01].治疗组TFH细胞比例(12.40％±2.726％)低于对照组(36.33％±2.750％),P<0.01.体外双氢青蒿素可抑制TFH细胞分化,0、0.1、1.0、10μmol/L双氢青蒿素剂量依赖性抑制TFH细胞百分比(分别为29.43％±1.94％、21.07％±1.19％、11.27％±1.40％、6.28％±1.02％;P<0.01),且抑制细胞因子IL-21的分泌[分别为(87.07±5.49)、(63.63±3.70)、(47.67±4.02)、(34.37±5.10)pg/mL,P<0.01].结论 双氢青蒿素对MRL/lpr狼疮鼠具有治疗作用,可有效改善狼疮肾炎、降低抗体滴度,其机制可能与抑制TFH细胞 |
| Author | 杨骥 薛美娟 黄俊霞 |
| AuthorAffiliation | 复旦大学附属中山医院皮肤科,上海 200032 |
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| Author_FL | XUE Mei-juan HUANG Jun-xia YANG Ji |
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| Keywords | 滤泡辅助性T细胞 双氢青蒿素 红斑狼疮 细胞分化 |
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