辣木叶酶法水解提取蛋白质工艺优化
TS201.1; 为了优化纤维素酶与果胶酶水解提取辣木叶中蛋白质的提取工艺,以提取率为考察指标,运用单因素与正交试验研究了酶解温度、加酶量、pH、底物质量浓度与酶解时间5 个因素对辣木叶蛋白质提取率的影响.结果表明:纤维素酶各因素对辣木叶蛋白质提取率影响的主次顺序为:酶解温度＞底物质量浓度＞pH＞酶解时间＞加酶量,最佳工艺条件为:酶解温度40℃、加酶量800 U/L、酶解pH5.0、底物质量浓度7.0 g/L、酶解时间70 min,在此条件下的提取率达到了 43.85％.果胶酶各因素对辣木叶蛋白质提取率影响的主次顺序为:加酶量＞底物质量浓度＞酶解时间＞酶解温度＞pH,最佳工艺条件为:酶解温度5...
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| Published in | 食品工业科技 Vol. 42; no. 5; pp. 194 - 199 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
云南省热带作物科学研究所,云南景洪666100%文山学院化学与工程学院,云南文山663000
01.03.2021
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1002-0306 |
| DOI | 10.13386/j.issn1002-0306.2020040110 |
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| Abstract | TS201.1; 为了优化纤维素酶与果胶酶水解提取辣木叶中蛋白质的提取工艺,以提取率为考察指标,运用单因素与正交试验研究了酶解温度、加酶量、pH、底物质量浓度与酶解时间5 个因素对辣木叶蛋白质提取率的影响.结果表明:纤维素酶各因素对辣木叶蛋白质提取率影响的主次顺序为:酶解温度＞底物质量浓度＞pH＞酶解时间＞加酶量,最佳工艺条件为:酶解温度40℃、加酶量800 U/L、酶解pH5.0、底物质量浓度7.0 g/L、酶解时间70 min,在此条件下的提取率达到了 43.85％.果胶酶各因素对辣木叶蛋白质提取率影响的主次顺序为:加酶量＞底物质量浓度＞酶解时间＞酶解温度＞pH,最佳工艺条件为:酶解温度50℃、加酶量1400 U/L、pH4.0、底物质量浓度9.0 g/L、酶解时间50 min,提取率达到了 32.26％.纤维素酶与果胶酶各因素对辣木叶蛋白质提取率的影响均达到了极显著水平(P＜0.01).在最佳工艺条件下,纤维素酶水解辣木叶提取蛋白质的效果优于果胶酶. |
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| AbstractList | TS201.1; 为了优化纤维素酶与果胶酶水解提取辣木叶中蛋白质的提取工艺,以提取率为考察指标,运用单因素与正交试验研究了酶解温度、加酶量、pH、底物质量浓度与酶解时间5 个因素对辣木叶蛋白质提取率的影响.结果表明:纤维素酶各因素对辣木叶蛋白质提取率影响的主次顺序为:酶解温度＞底物质量浓度＞pH＞酶解时间＞加酶量,最佳工艺条件为:酶解温度40℃、加酶量800 U/L、酶解pH5.0、底物质量浓度7.0 g/L、酶解时间70 min,在此条件下的提取率达到了 43.85％.果胶酶各因素对辣木叶蛋白质提取率影响的主次顺序为:加酶量＞底物质量浓度＞酶解时间＞酶解温度＞pH,最佳工艺条件为:酶解温度50℃、加酶量1400 U/L、pH4.0、底物质量浓度9.0 g/L、酶解时间50 min,提取率达到了 32.26％.纤维素酶与果胶酶各因素对辣木叶蛋白质提取率的影响均达到了极显著水平(P＜0.01).在最佳工艺条件下,纤维素酶水解辣木叶提取蛋白质的效果优于果胶酶. |
| Author | 李海泉 马尚玄 张祖兵 郭刚军 胡小静 徐荣 |
| AuthorAffiliation | 云南省热带作物科学研究所,云南景洪666100%文山学院化学与工程学院,云南文山663000 |
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| Author_FL | GUO Gangjun XU Rong LI Haiquan ZHANG Zubing HU Xiaojing MA Shangxuan |
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| Keywords | 提取率 辣木叶 果胶酶 纤维素酶 蛋白质 |
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| Publisher | 云南省热带作物科学研究所,云南景洪666100%文山学院化学与工程学院,云南文山663000 |
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| Title | 辣木叶酶法水解提取蛋白质工艺优化 |
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