蒿本内酯对葡聚糖硫酸钠诱导的溃疡性结肠炎小鼠的改善作用

R459.3; 目的:探究蒿本内酯(ligustilide,Lig)对葡聚糖硫酸钠(dextran sodium sulfate,DSS)诱导溃疡性结肠炎(ulcerative colitis,UC)小鼠的改善作用及机制.方法:将 60只雄性SPF级C57BL/6小鼠随机分成 6组,分别为空白组、DSS组、阳性药柳氮磺吡啶(sulfasalazine,SASP)组、Lig低、中、高剂量组.DSS组小鼠自由饮用3%DSS水溶液 7d复制UC模型,同时对Lig低、中、高剂量组小鼠灌胃给予药物干预治疗.通过记录小鼠每日体重和结肠长度,检测疾病活动指数(disease activity index,D...

Full description

Saved in:
Bibliographic Details
Published in食品工业科技 Vol. 45; no. 4; pp. 321 - 327
Main Authors 马广礼, 夏晓培, 马金亮
Format Journal Article
LanguageChinese
Published 许昌职业技术学医疗卫生学院,河南许昌 461000%河南科技大学食品与生物工程学院,河南洛阳 471000 01.02.2024
Subjects
Online AccessGet full text
ISSN1002-0306
DOI10.13386/j.issn1002-0306.2023040055

Cover

Abstract R459.3; 目的:探究蒿本内酯(ligustilide,Lig)对葡聚糖硫酸钠(dextran sodium sulfate,DSS)诱导溃疡性结肠炎(ulcerative colitis,UC)小鼠的改善作用及机制.方法:将 60只雄性SPF级C57BL/6小鼠随机分成 6组,分别为空白组、DSS组、阳性药柳氮磺吡啶(sulfasalazine,SASP)组、Lig低、中、高剂量组.DSS组小鼠自由饮用3%DSS水溶液 7d复制UC模型,同时对Lig低、中、高剂量组小鼠灌胃给予药物干预治疗.通过记录小鼠每日体重和结肠长度,检测疾病活动指数(disease activity index,DAI)评分,采用酶联免疫吸附测定试剂盒定量检测血清中TNF-α、IL-6和IL-1β的表达水平,检测结肠中TLR4/NF-κB蛋白表达水平,并结合苏木素-伊红染色实验,探究Lig对小鼠UC的作用及机制.结果:与空白组比较,DSS组小鼠体重显著减轻(P<0.05),结肠长度显著缩短(P<0.05),DAI评分显著升高(P<0.05),肠道出现大量炎症性浸润现象,且结肠组织中TNF-α、IL-6和IL-1β表达水平显著升高(P<0.05);与DSS组比较,Lig中剂量组和高剂量组小鼠的肠道组织中TNF-α、IL-6和IL-1β的表达以及TLR4/NF-κB信号均被显著抑制(P<0.05),表明Lig中剂量组和高剂量组小鼠肠道损伤得到显著改善.结论:Lig能有效改善DSS诱导的小鼠UC,其机制可能是抑制NF-κB信号通路的激活.
AbstractList R459.3; 目的:探究蒿本内酯(ligustilide,Lig)对葡聚糖硫酸钠(dextran sodium sulfate,DSS)诱导溃疡性结肠炎(ulcerative colitis,UC)小鼠的改善作用及机制.方法:将 60只雄性SPF级C57BL/6小鼠随机分成 6组,分别为空白组、DSS组、阳性药柳氮磺吡啶(sulfasalazine,SASP)组、Lig低、中、高剂量组.DSS组小鼠自由饮用3%DSS水溶液 7d复制UC模型,同时对Lig低、中、高剂量组小鼠灌胃给予药物干预治疗.通过记录小鼠每日体重和结肠长度,检测疾病活动指数(disease activity index,DAI)评分,采用酶联免疫吸附测定试剂盒定量检测血清中TNF-α、IL-6和IL-1β的表达水平,检测结肠中TLR4/NF-κB蛋白表达水平,并结合苏木素-伊红染色实验,探究Lig对小鼠UC的作用及机制.结果:与空白组比较,DSS组小鼠体重显著减轻(P<0.05),结肠长度显著缩短(P<0.05),DAI评分显著升高(P<0.05),肠道出现大量炎症性浸润现象,且结肠组织中TNF-α、IL-6和IL-1β表达水平显著升高(P<0.05);与DSS组比较,Lig中剂量组和高剂量组小鼠的肠道组织中TNF-α、IL-6和IL-1β的表达以及TLR4/NF-κB信号均被显著抑制(P<0.05),表明Lig中剂量组和高剂量组小鼠肠道损伤得到显著改善.结论:Lig能有效改善DSS诱导的小鼠UC,其机制可能是抑制NF-κB信号通路的激活.
Abstract_FL Objective:To research the therapeutic effects and mechanisms of ligustilide(Lig)in improving dextran sodium sulfate(DSS)-induced ulcerative colitis(UC)in mice.Methods:Intervention of lig was delivered in sixty male SPF C57 BL/6 mice and the mice were differentiated in six groups according to the dose of lig as:Control group,DSS group,positive control group(treated with sulfasalazine,SASP),low-dose lig group,medium-dose lig group,and high-dose lig group.The UC in DSS mice was induced by a 3%DSS solution through oral administration for 7 days,in the meanwhile,the low,medium,and high-dose lig groups were received gavage feeding of lig and oral administration of 3%DSS solution.Before and after the intervention,the body weight,colon length,and the disease activity index(DAI)score were collected for assessment of UC.Furthermore,the expression levels of TLR4/NF-κB proteins in colon and serum TNF-α,IL-6,and IL-1β were quantitatively measured using enzyme-linked immunosorbent(ELISA)assays.Additionally,histological exam-ination was performed to investigate the effects and mechanisms of lig in improving UC in mice by hematoxylin-eosin staining.Results:Compared to the control group,significantly reduced body weight(P<0.05),shorter colon length(P<0.05),increased DAI score(P<0.05).And there was a large amount of inflammatory infiltration in the intestine,the expression level of TNF-α,IL-6,and IL-1β in the colon significantly increased(P<0.05).Compared with DSS group,significant suppression of the TLR4/NF-κB signaling pathway in the intestinal tissue,serum TNF-α,IL-6,and IL-1β expression(P<0.05)were observed in the medium and high-dose lig groups,which indicated a significant improvement in intestinal damage.Conclusion:Lig would be able to effectively improve DSS-induced UC in mice.Moreover,the results showed that the mechanism of lig improving DSS-induced UC was possibly involved with the inhibition of the NF-κB signaling pathway.
Author 马金亮
马广礼
夏晓培
AuthorAffiliation 许昌职业技术学医疗卫生学院,河南许昌 461000%河南科技大学食品与生物工程学院,河南洛阳 471000
AuthorAffiliation_xml – name: 许昌职业技术学医疗卫生学院,河南许昌 461000%河南科技大学食品与生物工程学院,河南洛阳 471000
Author_FL MA Jinliang
MA Guangli
XIA Xiaopei
Author_FL_xml – sequence: 1
  fullname: MA Guangli
– sequence: 2
  fullname: XIA Xiaopei
– sequence: 3
  fullname: MA Jinliang
Author_xml – sequence: 1
  fullname: 马广礼
– sequence: 2
  fullname: 夏晓培
– sequence: 3
  fullname: 马金亮
BookMark eNo9j81Kw0AcxPdQwVr7FB48Jf43202yRyl-QUFE72WbTUqjpOIi4k1r67FiLTmUHtqTgkIUL1YrvozJJm9hiyJzGJgfzDBLKBc0AxehFQw6JsQ213y9IWWAAQwNCJi6AQaBEgClOZT_jxdRUcpGDQDjGaEkj_bS3lcyfIqvO1kniqNJejtOLwfqNVTjx6zzlvVGafQSR1M1aCfvVyocJxf36uMubY1Uqxs_32TT0Rz1J3HY_v4cqv7DMlrw-JF0i39eQPubGwflba2yu7VTXq9oEoMBGuG05lHb5Zx5tiUAe8JkjGEOAlNSc5lllxxmEZcKwQ1OTMNhwsHCdSh3KCmg1d_WMx54PKhX_ebpSTDbq8rj-vmhP_tfgrnID9GHcls
ClassificationCodes R459.3
ContentType Journal Article
Copyright Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
Copyright_xml – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
DBID 2B.
4A8
92I
93N
PSX
TCJ
DOI 10.13386/j.issn1002-0306.2023040055
DatabaseName Wanfang Data Journals - Hong Kong
WANFANG Data Centre
Wanfang Data Journals
万方数据期刊 - 香港版
China Online Journals (COJ)
China Online Journals (COJ)
DatabaseTitleList
DeliveryMethod fulltext_linktorsrc
Discipline Engineering
DocumentTitle_FL Improving Effect of Ligustilide on Dextran Sodium Sulfate-induced Ulcerative Colitis
EndPage 327
ExternalDocumentID spgykj202404040
GrantInformation_xml – fundername: 河南省高等教育教学改革研究与实践项目
  funderid: (2019SJGLX716)
GroupedDBID -02
2B.
4A8
5XA
5XC
92H
92I
93N
ABJNI
ACGFS
ALMA_UNASSIGNED_HOLDINGS
CCEZO
CDRFL
CW9
GROUPED_DOAJ
PSX
TCJ
TGT
U1G
U5L
ID FETCH-LOGICAL-s1020-3a5bf58eaa9f87d01fd69991a0d153be9784c973e5dda2a362c9dc1dec5ac53
ISSN 1002-0306
IngestDate Thu May 29 04:11:23 EDT 2025
IsPeerReviewed true
IsScholarly true
Issue 4
Keywords 蒿本内酯
inflammatory factor
溃疡性结肠炎
葡聚糖硫酸钠
炎症因子
ulcerative colitis
dextran sodium sulfate
ligustilide
Language Chinese
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-s1020-3a5bf58eaa9f87d01fd69991a0d153be9784c973e5dda2a362c9dc1dec5ac53
PageCount 7
ParticipantIDs wanfang_journals_spgykj202404040
PublicationCentury 2000
PublicationDate 2024-02-01
PublicationDateYYYYMMDD 2024-02-01
PublicationDate_xml – month: 02
  year: 2024
  text: 2024-02-01
  day: 01
PublicationDecade 2020
PublicationTitle 食品工业科技
PublicationTitle_FL Science and Technology of Food Industry
PublicationYear 2024
Publisher 许昌职业技术学医疗卫生学院,河南许昌 461000%河南科技大学食品与生物工程学院,河南洛阳 471000
Publisher_xml – name: 许昌职业技术学医疗卫生学院,河南许昌 461000%河南科技大学食品与生物工程学院,河南洛阳 471000
SSID ssib001105553
ssib051376492
ssj0050115
Score 2.3728995
Snippet R459.3; 目的:探究蒿本内酯(ligustilide,Lig)对葡聚糖硫酸钠(dextran sodium sulfate,DSS)诱导溃疡性结肠炎(ulcerative colitis,UC)小鼠的改善作用及机制.方法:将 60只雄...
SourceID wanfang
SourceType Aggregation Database
StartPage 321
Title 蒿本内酯对葡聚糖硫酸钠诱导的溃疡性结肠炎小鼠的改善作用
URI https://d.wanfangdata.com.cn/periodical/spgykj202404040
Volume 45
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
journalDatabaseRights – providerCode: PRVAON
  databaseName: DOAJ Directory of Open Access Journals
  issn: 1002-0306
  databaseCode: DOA
  dateStart: 20210101
  customDbUrl:
  isFulltext: true
  dateEnd: 99991231
  titleUrlDefault: https://www.doaj.org/
  omitProxy: true
  ssIdentifier: ssj0050115
  providerName: Directory of Open Access Journals
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Na9RAFB9qBdGD-InfFHROZWs-ZpKZ4yTNUjwIYoXeSr62UmEV2x7sSWvbo2ItPZQe2pOCwiperFb8Z9xm97_wvUk2G9eKVRaG2Zc37715vyTzJpl5IeRG6qSOa7G05mKeIRaHcU0aqQmBnGw0JIutMNLZPm87E_fYrSk-NTS8Wlm1tDAfjcWLB-4r-R9UgQa44i7Zf0C2FAoEqAO-UALCUB4KYxoIKi3q1WngUOlT5dOAU-FQwWkgsVR1pEDpSc1sUmViRZhUKhq41LOpdLACdOUVrTyBFZCsDGTG5mZPjo_M0FYwVOpBxdYUR0t2cOWEcrVkj0pb68rluFgRAcrxDCrqqAKk5YdKgZJpUzkKRAr8HceuIQ-jSlSDaZSgwH7dR9AltJEe9IXrhqLoI9gjc9tAS_l6RreWhc9AJ3oReKGd32fhSBC5f6XuD0d9njxACrguV8TQLSqoPlCxWG8NdnEJaL8G2tMgGpzkF7BgpbS9MLkHbw7mOFWOxtlHH-e-l66mjGsMtatklVmi9cqC60mDZiHsOT82HLRkFLPj49e7-UHcg_4snKTcX_QN4qK7hOiXxsH5oF3XhwxK4PEObTdQQJQ-acFuzx5lmETKqIyzOBDjdLU6EOd5RYsbDquMqna-ib4I0Ow8mcRvY79t63eRs1pDqWDMwlk2w1xz_ZCnXIg692jmyYNZPAsM_B0hRy2IDozKoxk9rcCvyPbDWm7CqMxkmaaO47RKr6oodB4j13sG3fyzOXqbYLMRNmcqEe3kKXKymIqOqPy-cpoMLd4_Q05UEpSeJXc6a9_3t963V1e6K612a7fzaqfzbDP7tJHtvOuufO6ubXdaH9utvWxzef_L82xjZ__pm-zr687Sdrb0ov3hZXdvGw-t77Y3ln9828rW354jd-vBpD9RKz7CUpsz8dGSHfKowUUahrIh3MQwG4mDk8rQSCBYilLpChZL1055koRWCPFwLJPYTNKYhzG3z5Ph5sNmeoGMRI4rwzjhwByx0HVkBKN_wiV-HSCMjfgiGSncMV3cYuemB-C59HeWy-R4_5q-QobnHy-kV2HaMB9d05j-BK0xz5M
linkProvider Directory of Open Access Journals
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E8%92%BF%E6%9C%AC%E5%86%85%E9%85%AF%E5%AF%B9%E8%91%A1%E8%81%9A%E7%B3%96%E7%A1%AB%E9%85%B8%E9%92%A0%E8%AF%B1%E5%AF%BC%E7%9A%84%E6%BA%83%E7%96%A1%E6%80%A7%E7%BB%93%E8%82%A0%E7%82%8E%E5%B0%8F%E9%BC%A0%E7%9A%84%E6%94%B9%E5%96%84%E4%BD%9C%E7%94%A8&rft.jtitle=%E9%A3%9F%E5%93%81%E5%B7%A5%E4%B8%9A%E7%A7%91%E6%8A%80&rft.au=%E9%A9%AC%E5%B9%BF%E7%A4%BC&rft.au=%E5%A4%8F%E6%99%93%E5%9F%B9&rft.au=%E9%A9%AC%E9%87%91%E4%BA%AE&rft.date=2024-02-01&rft.pub=%E8%AE%B8%E6%98%8C%E8%81%8C%E4%B8%9A%E6%8A%80%E6%9C%AF%E5%AD%A6%E5%8C%BB%E7%96%97%E5%8D%AB%E7%94%9F%E5%AD%A6%E9%99%A2%2C%E6%B2%B3%E5%8D%97%E8%AE%B8%E6%98%8C+461000%25%E6%B2%B3%E5%8D%97%E7%A7%91%E6%8A%80%E5%A4%A7%E5%AD%A6%E9%A3%9F%E5%93%81%E4%B8%8E%E7%94%9F%E7%89%A9%E5%B7%A5%E7%A8%8B%E5%AD%A6%E9%99%A2%2C%E6%B2%B3%E5%8D%97%E6%B4%9B%E9%98%B3+471000&rft.issn=1002-0306&rft.volume=45&rft.issue=4&rft.spage=321&rft.epage=327&rft_id=info:doi/10.13386%2Fj.issn1002-0306.2023040055&rft.externalDocID=spgykj202404040
thumbnail_s http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fspgykj%2Fspgykj.jpg