红茶菌菌相分析及优势菌的分离与鉴定

目的:了解红茶菌菌相结构及获取发酵菌株.方法:采用16S rDNA高通量测序技术和传统微生物培养法对红茶菌菌相组成和优势菌株进行分析.结果:高通量测序显示红茶菌的细菌群落丰富度与多样性大于真菌.结论:细菌中驹形氏杆菌(Kohmagataeibacter)、醋酸杆菌属(Acetobacter)、乳杆菌属(Lactobacillus)为优势菌株,真菌中德克酵母属(Dekkera)、接合酵母属(Zygosaccha-ormyces)为优势菌株.传统平板培养法分离得到7株醋酸菌、3株酵母菌和1株乳酸菌,其中食糖驹形氏杆菌(Komagataeibacter saccharivorans)1 株、腐烂苹果...

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Published in食品与机械 Vol. 38; no. 4; pp. 47 - 53
Main Authors 徐素云, 李佳佳, 方伟, 王艳萍, 耿伟涛
Format Journal Article
LanguageChinese
Published 天津科技大学食品工程与生物技术学院,天津 300457 2022
Subjects
高通量测序
红茶菌
菌相
微生物平板培养法
优势菌
Online AccessGet full text
ISSN1003-5788
DOI10.13652/j.spjx.1003.5788.2022.90134

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Abstract 目的:了解红茶菌菌相结构及获取发酵菌株.方法:采用16S rDNA高通量测序技术和传统微生物培养法对红茶菌菌相组成和优势菌株进行分析.结果:高通量测序显示红茶菌的细菌群落丰富度与多样性大于真菌.结论:细菌中驹形氏杆菌(Kohmagataeibacter)、醋酸杆菌属(Acetobacter)、乳杆菌属(Lactobacillus)为优势菌株,真菌中德克酵母属(Dekkera)、接合酵母属(Zygosaccha-ormyces)为优势菌株.传统平板培养法分离得到7株醋酸菌、3株酵母菌和1株乳酸菌,其中食糖驹形氏杆菌(Komagataeibacter saccharivorans)1 株、腐烂苹果醋酸杆菌(Acetobacter malorum)1 株、木驹形氏菌(Komaga-taeibacter xylinus)4 株、醋酸杆菌属(Acetobacter)1 株、拜耳接合酵母(Zygosaccharomyces bailii)2株、布鲁塞尔德克酒香酵母(Dekkera bruxellensis)1株、植物乳杆菌(Lactobacillus.plantarum)1 株.
AbstractList 目的:了解红茶菌菌相结构及获取发酵菌株.方法:采用16S rDNA高通量测序技术和传统微生物培养法对红茶菌菌相组成和优势菌株进行分析.结果:高通量测序显示红茶菌的细菌群落丰富度与多样性大于真菌.结论:细菌中驹形氏杆菌(Kohmagataeibacter)、醋酸杆菌属(Acetobacter)、乳杆菌属(Lactobacillus)为优势菌株,真菌中德克酵母属(Dekkera)、接合酵母属(Zygosaccha-ormyces)为优势菌株.传统平板培养法分离得到7株醋酸菌、3株酵母菌和1株乳酸菌,其中食糖驹形氏杆菌(Komagataeibacter saccharivorans)1 株、腐烂苹果醋酸杆菌(Acetobacter malorum)1 株、木驹形氏菌(Komaga-taeibacter xylinus)4 株、醋酸杆菌属(Acetobacter)1 株、拜耳接合酵母(Zygosaccharomyces bailii)2株、布鲁塞尔德克酒香酵母(Dekkera bruxellensis)1株、植物乳杆菌(Lactobacillus.plantarum)1 株.
Author 耿伟涛
方伟
李佳佳
徐素云
王艳萍
AuthorAffiliation 天津科技大学食品工程与生物技术学院,天津 300457
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FANG Wei
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红茶菌
菌相
微生物平板培养法
优势菌
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Snippet 目的:了解红茶菌菌相结构及获取发酵菌株.方法:采用16S rDNA高通量测序技术和传统微生物培养法对红茶菌菌相组成和优势菌株进行分析.结果:高通量测序显示红茶菌的细菌群落丰...
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