苯酚高效降解菌群的群落结构及其代谢产物研究

X703.1; 采用焦化厂好氧池活性污泥,通过分离筛选和驯化,成功得到了高效苯酚降解菌群(PDB),探究其对水相中苯酚降解的效果.结果表明,PDB菌群对苯酚的降解效果受底物质量浓度影响,当苯酚质量浓度低于700 mg/L时,苯酚降解率达到100%;当苯酚质量浓度增加至1 300 mg/L时,降解率降至41.6%;当苯酚质量浓度高于1 700 mg/L时,PDB菌群基本不能生长.通过高通量测序发现,PDB菌群物种丰富度较高,其中Proteobacteria、Firmicutes和Bacteroidota是优势门,Serratia sp.、Bacillus sp.和Muribaculaceae s...

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Published in工业水处理 Vol. 44; no. 9; pp. 85 - 90
Main Authors 徐玲莉, 刘玉香, 董静, 任莉, 王文君, 袁可
Format Journal Article
LanguageChinese
Published 太原理工大学环境与生态学院,山西晋中 030600%太原理工大学环境与生态学院,山西晋中 030600 01.09.2024
哈尔滨工业大学城市水资源与水环境国家重点实验室,黑龙江哈尔滨 150090
Subjects
微生物降解
community structure
代谢产物
microbial degradation
phenol
苯酚
metabolites
群落结构
Online AccessGet full text
ISSN1005-829X
DOI10.19965/j.cnki.iwt.2023-0885

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Abstract X703.1; 采用焦化厂好氧池活性污泥,通过分离筛选和驯化,成功得到了高效苯酚降解菌群(PDB),探究其对水相中苯酚降解的效果.结果表明,PDB菌群对苯酚的降解效果受底物质量浓度影响,当苯酚质量浓度低于700 mg/L时,苯酚降解率达到100%;当苯酚质量浓度增加至1 300 mg/L时,降解率降至41.6%;当苯酚质量浓度高于1 700 mg/L时,PDB菌群基本不能生长.通过高通量测序发现,PDB菌群物种丰富度较高,其中Proteobacteria、Firmicutes和Bacteroidota是优势门,Serratia sp.、Bacillus sp.和Muribaculaceae sp.是核心优势菌属.通过气相色谱-质谱联用(GC-MC)分析代谢产物可知,PDB菌群降解苯酚的路径是邻位开环裂解方式,产生酸性中间产物,随后分解为链状小分子物质.
AbstractList X703.1; 采用焦化厂好氧池活性污泥,通过分离筛选和驯化,成功得到了高效苯酚降解菌群(PDB),探究其对水相中苯酚降解的效果.结果表明,PDB菌群对苯酚的降解效果受底物质量浓度影响,当苯酚质量浓度低于700 mg/L时,苯酚降解率达到100%;当苯酚质量浓度增加至1 300 mg/L时,降解率降至41.6%;当苯酚质量浓度高于1 700 mg/L时,PDB菌群基本不能生长.通过高通量测序发现,PDB菌群物种丰富度较高,其中Proteobacteria、Firmicutes和Bacteroidota是优势门,Serratia sp.、Bacillus sp.和Muribaculaceae sp.是核心优势菌属.通过气相色谱-质谱联用(GC-MC)分析代谢产物可知,PDB菌群降解苯酚的路径是邻位开环裂解方式,产生酸性中间产物,随后分解为链状小分子物质.
Abstract_FL By using activated sludge from the anaerobic tank of a coking plant,a highly efficient phenol-degrading bacterial(PDB)community was successfully obtained through separation and domestication,and its effect on micro-bial degradation of phenol was investigated.The results showed that the degradation effect of PDB community on phenol was affected by the substrate concentration.The degradation rate reached 100%when the phenol concentra-tion was lower than 700 mg/L.However,when the concentration of phenol increased to 1 300 mg/L,the degradation rate decreased to 41.6%.When the concentration of phenol exceeded 1 700 mg/L,the PDB community could basi-cally unable to grow.High-throughput sequencing revealed that the species richness within the PDB community was high.Proteobacteria,Firmicutes,and Bacteroidota were dominant phyla,while Serratia sp.,Bacillus sp.,and Murib-aculaceae sp.were core dominant genera of PDB.Metabolites analyzed by gas chromatography-mass spectrometry(GC-MS)revealed that the pathway for phenol degradation by PDB community was through ortho-cleavage,resulting in acidic intermediate products that subsequently decomposed into chain-like small molecular substances.
Author 王文君
徐玲莉
刘玉香
任莉
董静
袁可
AuthorAffiliation 太原理工大学环境与生态学院,山西晋中 030600%太原理工大学环境与生态学院,山西晋中 030600;哈尔滨工业大学城市水资源与水环境国家重点实验室,黑龙江哈尔滨 150090
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Author_FL XU Lingli
DONG Jing
WANG Wenjun
LIU Yuxiang
REN Li
YUAN Ke
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Keywords 微生物降解
community structure
代谢产物
microbial degradation
phenol
苯酚
metabolites
群落结构
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哈尔滨工业大学城市水资源与水环境国家重点实验室,黑龙江哈尔滨 150090
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Title 苯酚高效降解菌群的群落结构及其代谢产物研究
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