稀土荧光探针用于快速检测乙肝表面抗原

O655; 采用自合成的稀土螯合剂4,4′-双(1″,1″,1″,2″,2″,3″,3″-7氟-4″,6″-己二酮-6″-基)氯磺化三联苯(BHHCT)制备乙肝表面抗体标记物,应用侧流免疫层析试纸条和配套的时间分辨荧光检测系统,建立了一种时间分辨荧光免疫分析对乙肝表面抗原(HBsAg)快速检测的方法.该方法线性范围为0.2～500 ng/mL,检出限为0.16 ng/mL,线性回归决定系数(R2)为0.9996.批内变异系数小于5％,批间变异系数小于8％,样品回收率94.3％～107.8％.该方法操作简便快捷,20 min内获得检测结果,具有精准、快速、特异性强和无需专业人员操作等优势,满足了...

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Published in应用化学 Vol. 41; no. 12; pp. 1760 - 1769
Main Authors 张瑞起, 常宇, 刘作家, 李雪松, 潘利华
Format Journal Article
LanguageChinese
Published 长春工业大学材料科学与工程学院,先进结构材料教育部重点实验室,长春 130012%中国科学院长春应用化学研究所,长春 130022 01.12.2024
Subjects
时间分辨荧光免疫分析法
Lateral flow immunochromatography strip
Hepatitis B surface antigen
即时检测
侧流免疫层析试纸条
Point of care testing
Time-resolved fluorescence immunoassay
乙肝表面抗原
Online AccessGet full text
ISSN1000-0518
DOI10.19894/j.issn.1000-0518.240055

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Abstract O655; 采用自合成的稀土螯合剂4,4′-双(1″,1″,1″,2″,2″,3″,3″-7氟-4″,6″-己二酮-6″-基)氯磺化三联苯(BHHCT)制备乙肝表面抗体标记物,应用侧流免疫层析试纸条和配套的时间分辨荧光检测系统,建立了一种时间分辨荧光免疫分析对乙肝表面抗原(HBsAg)快速检测的方法.该方法线性范围为0.2～500 ng/mL,检出限为0.16 ng/mL,线性回归决定系数(R2)为0.9996.批内变异系数小于5％,批间变异系数小于8％,样品回收率94.3％～107.8％.该方法操作简便快捷,20 min内获得检测结果,具有精准、快速、特异性强和无需专业人员操作等优势,满足了乙肝表面抗原的即时检测的精准、快速要求.
AbstractList O655; 采用自合成的稀土螯合剂4,4′-双(1″,1″,1″,2″,2″,3″,3″-7氟-4″,6″-己二酮-6″-基)氯磺化三联苯(BHHCT)制备乙肝表面抗体标记物,应用侧流免疫层析试纸条和配套的时间分辨荧光检测系统,建立了一种时间分辨荧光免疫分析对乙肝表面抗原(HBsAg)快速检测的方法.该方法线性范围为0.2～500 ng/mL,检出限为0.16 ng/mL,线性回归决定系数(R2)为0.9996.批内变异系数小于5％,批间变异系数小于8％,样品回收率94.3％～107.8％.该方法操作简便快捷,20 min内获得检测结果,具有精准、快速、特异性强和无需专业人员操作等优势,满足了乙肝表面抗原的即时检测的精准、快速要求.
Abstract_FL In this study,a self-synthesized rare-earth chelator 4,4′-Bis(1″,1″,1″,2″,2″,3″,3″-7-fluoro-4″,6″-hexanedione-6″-yl)chlorosulfonated terphenyl (BHHCT) was used to prepare hepatitis B surface antibody markers,and a time-resolved fluorescence immunoassay for the rapid detection of hepatitis B surface antigen (HBsAg) was developed by applying lateral-flow immunochromatographic test strips and an accompanying time-resolved fluorescence detection system. The linear range was 0.2～500 ng/mL,the detection limit was 0.16 ng/mL,and the linear regression coefficient of determination (R2) was 0.9996. The intra-batch coefficient of variation was less than 5％,the inter-batch coefficient of variation was less than 8％,and the recoveries of the samples ranged from 94.3％ to 107.8％. The method has the advantages of precision,rapidity,specificity and no need for specialized personnel to operate,which can meet the requirement of immediate detection of hepatitis B surface antigen.
Author 潘利华
张瑞起
常宇
李雪松
刘作家
AuthorAffiliation 长春工业大学材料科学与工程学院,先进结构材料教育部重点实验室,长春 130012%中国科学院长春应用化学研究所,长春 130022
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Author_FL CHANG Yu
LI Xue-Song
PAN Li-Hua
ZHANG Rui-Qi
LIU Zuo-Jia
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Issue 12
Keywords 时间分辨荧光免疫分析法
Lateral flow immunochromatography strip
Hepatitis B surface antigen
即时检测
侧流免疫层析试纸条
Point of care testing
Time-resolved fluorescence immunoassay
乙肝表面抗原
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PublicationTitle 应用化学
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Publisher 长春工业大学材料科学与工程学院,先进结构材料教育部重点实验室,长春 130012%中国科学院长春应用化学研究所,长春 130022
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