高产酯化酶红曲霉的筛选、鉴定及其产酶条件优化
Q93-31; 该研究采用传统培养分离法从大曲、红曲米、酒糟样品中分离红曲霉(Monascus)菌株,通过透明圈法及酯化酶活力测定从中筛选高产酯化酶菌株,并通过形态观察及分子生物学技术对其进行菌种鉴定.在发酵培养基优化的基础上,以酯化酶活力为响应值,采用单因素及响应面试验对其发酵条件进行优化,并对所产酯化酶底物特异性进行研究.结果表明,筛选得到一株高产酯化酶的红曲霉菌株XTQ,其酯化酶活力为(43.08±0.266)U/L.经鉴定,该菌株为紫色红曲霉(Monascus purpureus).菌株XTQ产酯化酶最优液态培养基成分为葡萄糖50 g/L、牛肉浸粉20 g/L、硫酸镁1 g/L、磷酸二...
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| Published in | 中国酿造 Vol. 43; no. 12; pp. 103 - 109 |
|---|---|
| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
湖北工业大学生命科学与健康工程学院,湖北武汉 430068%湖北艾合菊科技有限责任公司,湖北黄冈 438300
25.12.2024
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0254-5071 |
| DOI | 10.11882/j.issn.0254-5071.2024.12.015 |
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| Abstract | Q93-31; 该研究采用传统培养分离法从大曲、红曲米、酒糟样品中分离红曲霉(Monascus)菌株,通过透明圈法及酯化酶活力测定从中筛选高产酯化酶菌株,并通过形态观察及分子生物学技术对其进行菌种鉴定.在发酵培养基优化的基础上,以酯化酶活力为响应值,采用单因素及响应面试验对其发酵条件进行优化,并对所产酯化酶底物特异性进行研究.结果表明,筛选得到一株高产酯化酶的红曲霉菌株XTQ,其酯化酶活力为(43.08±0.266)U/L.经鉴定,该菌株为紫色红曲霉(Monascus purpureus).菌株XTQ产酯化酶最优液态培养基成分为葡萄糖50 g/L、牛肉浸粉20 g/L、硫酸镁1 g/L、磷酸二氢钠1 g/L;最优发酵条件为初始pH值6.0、发酵温度33.4 ℃、转速185r/min.在最佳条件下,酯化酶活力达(93.82±5.65)U/L,是优化前的2.18倍.菌株XTQ所产酯化酶对己酸有良好的选择性,为合成己酸乙酯酯化酶的生产提供了参考. |
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| AbstractList | Q93-31; 该研究采用传统培养分离法从大曲、红曲米、酒糟样品中分离红曲霉(Monascus)菌株,通过透明圈法及酯化酶活力测定从中筛选高产酯化酶菌株,并通过形态观察及分子生物学技术对其进行菌种鉴定.在发酵培养基优化的基础上,以酯化酶活力为响应值,采用单因素及响应面试验对其发酵条件进行优化,并对所产酯化酶底物特异性进行研究.结果表明,筛选得到一株高产酯化酶的红曲霉菌株XTQ,其酯化酶活力为(43.08±0.266)U/L.经鉴定,该菌株为紫色红曲霉(Monascus purpureus).菌株XTQ产酯化酶最优液态培养基成分为葡萄糖50 g/L、牛肉浸粉20 g/L、硫酸镁1 g/L、磷酸二氢钠1 g/L;最优发酵条件为初始pH值6.0、发酵温度33.4 ℃、转速185r/min.在最佳条件下,酯化酶活力达(93.82±5.65)U/L,是优化前的2.18倍.菌株XTQ所产酯化酶对己酸有良好的选择性,为合成己酸乙酯酯化酶的生产提供了参考. |
| Abstract_FL | In this study,Monascus strains were isolated from Daqu,Hongqu rice and distillers'grains samples by traditional culture and separation method,and the high-yield esterase strain was screened by transparent circle method and esterase activity measurement and identified by morphologic observation and molecular biology techniques.On the basis of fermentation medium optimization,the fermentation conditions were optimized by sin-gle factor and response surface experiments using esterase activity as response value,and the substrate specificity of the esterase produced by the screened strain was investigated.The results showed that a high-yield esterase strain of Monascus XTQ was screened,with the esterase activity(43.08±0.266)U/L,which was identified as Monascus purpureus.The optimal esterase-producing liquid medium components for the strain XTQ were obtained as follows:glucose 50 g/L,beef extract 20 g/L,MgSO4 1 g/L,and NaH2SO4 1 g/L,and the optimal fermentation conditions were as fol-lows:pH 6.0,fermentation temperature 33.4 ℃,and rotational speed 185 r/min.Under these optimal conditions,the activity of esterase was up to(93.82±5.65)U/L,which was 2.18 times of that before optimization.The esterase produced by the strain XTQ had good selectivity for hexanoic acid,which provided a reference for the production of synthetic ethyl hexanoate esterase. |
| Author | 朱俊颖 夏芊芊 余登洋 张玉 丁保坤 陈茂彬 甘晋铭 |
| AuthorAffiliation | 湖北工业大学生命科学与健康工程学院,湖北武汉 430068%湖北艾合菊科技有限责任公司,湖北黄冈 438300 |
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| Author_FL | ZHANG Yu ZHU Junying GAN Jinming DING Baokun XIA Qianqian CHEN Maobin YU Dengyang |
| Author_FL_xml | – sequence: 1 fullname: ZHU Junying – sequence: 2 fullname: XIA Qianqian – sequence: 3 fullname: GAN Jinming – sequence: 4 fullname: YU Dengyang – sequence: 5 fullname: DING Baokun – sequence: 6 fullname: CHEN Maobin – sequence: 7 fullname: ZHANG Yu |
| Author_xml | – sequence: 1 fullname: 朱俊颖 – sequence: 2 fullname: 夏芊芊 – sequence: 3 fullname: 甘晋铭 – sequence: 4 fullname: 余登洋 – sequence: 5 fullname: 丁保坤 – sequence: 6 fullname: 陈茂彬 – sequence: 7 fullname: 张玉 |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DOI | 10.11882/j.issn.0254-5071.2024.12.015 |
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| DocumentTitle_FL | Screening,identification and enzyme production conditions optimization of high-yield esterase Monascus |
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| Keywords | 筛选 screening 酯化酶 Monascus 红曲霉 发酵条件 response surface method identification 鉴定 substrate specificity 响应面法 底物特异性 esterase fermentation condition |
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| Snippet | Q93-31; 该研究采用传统培养分离法从大曲、红曲米、酒糟样品中分离红曲霉(Monascus)菌株,通过透明圈法及酯化酶活力测定从中筛选高产酯化酶菌株,并通过形态观察及分子生物学... |
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| Title | 高产酯化酶红曲霉的筛选、鉴定及其产酶条件优化 |
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