HPLC-MS/MS法测定烟叶中9种B族维生素

TS411.1; 为准确测定烟叶样品中水溶性B族维生素的含量(质量分数),通过优化色谱流动相pH、柱温、提取剂及其体积等参数,建立了同时测定烟叶中9种B族维生素的高效液相色谱-三重四极杆质谱(HPLC-MS/MS)方法,并采用该方法对3个不同产区的烟叶样品进行了测定.结果表明:①在线性范围内,9种B族维生素工作曲线的线性关系良好,决定系数(R2)为0.9946～0.9999.②该方法的回收率为91.00％ ～108.79％,日内和日间精密度(RSD)为1.25％ ～9.73％,9种B族维生素的检出限为0.01～0.50 ng/mL.③3个产区烟叶中B族维生素含量有一定差异,其中河南襄城县产区烟...

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Published in烟草科技 Vol. 53; no. 7; pp. 33 - 39
Main Authors 刘萍萍, 申娟娟, 卢紫舒, 李泽锋, 张慧, 徐国云, 王晨, 金立锋, 曹培健, 周会娜, 陈千思
Format Journal Article
LanguageChinese
Published 中国烟草总公司郑州烟草研究院,郑州高新技术产业开发区枫杨街2号,450001%郑州大学化学与分子工程学院,郑州高新技术产业开发区科学大道100号,450001 01.07.2020
Subjects
B族维生素
烟叶
高效液相色谱-串联质谱(HPLC-MS/MS)
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ISSN1002-0861
DOI10.16135/j.issn1002-0861.2019.0011

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Abstract TS411.1; 为准确测定烟叶样品中水溶性B族维生素的含量(质量分数),通过优化色谱流动相pH、柱温、提取剂及其体积等参数,建立了同时测定烟叶中9种B族维生素的高效液相色谱-三重四极杆质谱(HPLC-MS/MS)方法,并采用该方法对3个不同产区的烟叶样品进行了测定.结果表明:①在线性范围内,9种B族维生素工作曲线的线性关系良好,决定系数(R2)为0.9946～0.9999.②该方法的回收率为91.00％ ～108.79％,日内和日间精密度(RSD)为1.25％ ～9.73％,9种B族维生素的检出限为0.01～0.50 ng/mL.③3个产区烟叶中B族维生素含量有一定差异,其中河南襄城县产区烟叶中VB2、VB7、VB9的含量较高,贵州贵阳市产区烟叶中VB3的含量较高.该方法操作简便,精密度较好,灵敏度较高,适用于烟叶中多种B族维生素的同时分析.
AbstractList TS411.1; 为准确测定烟叶样品中水溶性B族维生素的含量(质量分数),通过优化色谱流动相pH、柱温、提取剂及其体积等参数,建立了同时测定烟叶中9种B族维生素的高效液相色谱-三重四极杆质谱(HPLC-MS/MS)方法,并采用该方法对3个不同产区的烟叶样品进行了测定.结果表明:①在线性范围内,9种B族维生素工作曲线的线性关系良好,决定系数(R2)为0.9946～0.9999.②该方法的回收率为91.00％ ～108.79％,日内和日间精密度(RSD)为1.25％ ～9.73％,9种B族维生素的检出限为0.01～0.50 ng/mL.③3个产区烟叶中B族维生素含量有一定差异,其中河南襄城县产区烟叶中VB2、VB7、VB9的含量较高,贵州贵阳市产区烟叶中VB3的含量较高.该方法操作简便,精密度较好,灵敏度较高,适用于烟叶中多种B族维生素的同时分析.
Author 申娟娟
卢紫舒
王晨
金立锋
徐国云
曹培健
刘萍萍
张慧
周会娜
陈千思
李泽锋
AuthorAffiliation 中国烟草总公司郑州烟草研究院,郑州高新技术产业开发区枫杨街2号,450001%郑州大学化学与分子工程学院,郑州高新技术产业开发区科学大道100号,450001
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Author_FL LU Zishu
ZHANG Hui
WANG Chen
ZHOU Huina
LI Zefeng
SHEN Juanjuan
CAO Peijian
CHEN Qiansi
LIU Pingping
XU Guoyun
JIN Lifeng
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DocumentTitle_FL Determination of nine B vitamins in tobacco leaves by high performance liquid chromatography-tandem mass spectrometry
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