敲低乙酰辅酶A羧化酶1通过上调热休克蛋白27促进食管鳞状细胞癌迁移
目的:探讨敲低乙酰辅酶A羧化酶1(ACC1)对食管鳞状细胞癌(以下简称食管鳞癌)KYSE－450细胞迁移的影响及分子机制。方法:慢病毒转染法建立对照组细胞系sh－NC和敲低ACC1的细胞系sh－ACC1，脂质体法转染人热休克蛋白27(HSP27) siRNA建立对照及敲低HSP27的细胞系si－NC和si－HSP27，选择性乙酰基转移酶p300/CBP抑制剂C646抑制组蛋白乙酰化并设置对照组DMSO。Transwell法检测细胞迁移能力，实时荧光定量聚合酶链反应检测HSP27 mRNA的表达，Western blot检测ACC1、H3K9ac、HSP27和上皮－间充质转化相关蛋白E－cadh...
Saved in:
| Published in | 中华肿瘤杂志 Vol. 45; no. 6; pp. 482 - 489 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
新乡医学院第一附属医院胸外科，河南省卫辉市 453100%新乡医学院第一附属医院生命科学研究中心，河南省卫辉市 453100
01.06.2023
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 0253-3766 |
| DOI | 10.3760/cma.j.cn112152-20210517-00383 |
Cover
| Abstract | 目的:探讨敲低乙酰辅酶A羧化酶1(ACC1)对食管鳞状细胞癌(以下简称食管鳞癌)KYSE－450细胞迁移的影响及分子机制。方法:慢病毒转染法建立对照组细胞系sh－NC和敲低ACC1的细胞系sh－ACC1，脂质体法转染人热休克蛋白27(HSP27) siRNA建立对照及敲低HSP27的细胞系si－NC和si－HSP27，选择性乙酰基转移酶p300/CBP抑制剂C646抑制组蛋白乙酰化并设置对照组DMSO。Transwell法检测细胞迁移能力，实时荧光定量聚合酶链反应检测HSP27 mRNA的表达，Western blot检测ACC1、H3K9ac、HSP27和上皮－间充质转化相关蛋白E－cadherin和Vimentin的表达。结果:sh－NC组细胞中ACC1的表达水平高于sh－ACC1组(
P<0.01)。sh－NC组细胞迁移数[(159.00±24.38)个]低于sh－ACC1组[(361.80±26.81)个，
P<0.01]，sh－NC组细胞中E－cadherin蛋白表达水平高于sh－ACC1组，Vimentin蛋白表达水平低于sh－ACC1组(均
P<0.05)。sh－NC+si－NC组细胞迁移数[(189.20±16.02)个]低于sh－ACC1+si－NC组[(371.60±38.40)个，
P<0.01]，sh－NC+si－NC组细胞迁移数高于sh－NC+si－HSP27组[(83.80±24.38)个，
P<0.01]，sh－ACC1+si－NC组迁移细胞数高于sh－ACC1+si－HSP27组[(152.40±24.30)个，
P<0.01]。sh－NC+si－NC组细胞中E－cadherin蛋白表达水平低于sh－NC+si－HSP27组，高于sh－ACC1+si－NC组，Vimentin蛋白表达水平高于sh－NC+si－HSP27组，低于sh－ACC1+si－NC组(均
P<0.05)；sh－ACC1+si－NC组细胞中E－cadherin、Vimentin蛋白表达水平与sh－ACC1+si－HSP27组比较，差异有统计学意义均(均
P<0.01)。C646 20 μmmo/L处理细胞24 h后，sh－NC+DMSO组细胞迁移数[(190.80±11.95)个]低于sh－ACC1+DMSO组[(395.00±17.10)个，
P<0.01]，sh－NC+DMSO组细胞迁移数低于sh－NC+C6 |
|---|---|
| AbstractList | 目的:探讨敲低乙酰辅酶A羧化酶1(ACC1)对食管鳞状细胞癌(以下简称食管鳞癌)KYSE－450细胞迁移的影响及分子机制。方法:慢病毒转染法建立对照组细胞系sh－NC和敲低ACC1的细胞系sh－ACC1，脂质体法转染人热休克蛋白27(HSP27) siRNA建立对照及敲低HSP27的细胞系si－NC和si－HSP27，选择性乙酰基转移酶p300/CBP抑制剂C646抑制组蛋白乙酰化并设置对照组DMSO。Transwell法检测细胞迁移能力，实时荧光定量聚合酶链反应检测HSP27 mRNA的表达，Western blot检测ACC1、H3K9ac、HSP27和上皮－间充质转化相关蛋白E－cadherin和Vimentin的表达。结果:sh－NC组细胞中ACC1的表达水平高于sh－ACC1组(
P<0.01)。sh－NC组细胞迁移数[(159.00±24.38)个]低于sh－ACC1组[(361.80±26.81)个，
P<0.01]，sh－NC组细胞中E－cadherin蛋白表达水平高于sh－ACC1组，Vimentin蛋白表达水平低于sh－ACC1组(均
P<0.05)。sh－NC+si－NC组细胞迁移数[(189.20±16.02)个]低于sh－ACC1+si－NC组[(371.60±38.40)个，
P<0.01]，sh－NC+si－NC组细胞迁移数高于sh－NC+si－HSP27组[(83.80±24.38)个，
P<0.01]，sh－ACC1+si－NC组迁移细胞数高于sh－ACC1+si－HSP27组[(152.40±24.30)个，
P<0.01]。sh－NC+si－NC组细胞中E－cadherin蛋白表达水平低于sh－NC+si－HSP27组，高于sh－ACC1+si－NC组，Vimentin蛋白表达水平高于sh－NC+si－HSP27组，低于sh－ACC1+si－NC组(均
P<0.05)；sh－ACC1+si－NC组细胞中E－cadherin、Vimentin蛋白表达水平与sh－ACC1+si－HSP27组比较，差异有统计学意义均(均
P<0.01)。C646 20 μmmo/L处理细胞24 h后，sh－NC+DMSO组细胞迁移数[(190.80±11.95)个]低于sh－ACC1+DMSO组[(395.00±17.10)个，
P<0.01]，sh－NC+DMSO组细胞迁移数低于sh－NC+C6 |
| Abstract_FL | Objective:To investigate the effect of acetyl-CoA carboxylase 1 (ACC1) knockdown on the migration of esophageal squamous cell carcinoma (ESCC) KYSE-450 cell and underlying mechanism.Methods:Lentiviral transfection was conducted to establish sh-NC control cell and ACC1 knocking down cell (sh-ACC1). Human siRNA HSP27 and control were transfected by Lipo2000 to get si-HSP27 and si-NC. The selective acetyltransferase P300/CBP inhibitor C646 was used to inhibit histone acetylation and DMSO was used as vehicle control. Transwell assay was performed to detect cell migration. The expression of HSP27 mRNA was examined by reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) and the expressions of ACC1, H3K9ac, HSP27 and epithelial-mesenchymal transition-related proteins E-cadherin and Vimentin were detected by western blot.Results:The expression level of ACC1 in sh-NC group was higher than that in sh-ACC1 group (
P<0.01). The number of cell migration in sh-NC group was (159.00±24.38), lower than (361.80±26.81) in sh-ACC1 group (
P<0.01). The protein expression levels of E-cadherin and Vimentin in sh-NC group were statistically significant compared with sh-AAC1 group (
P<0.05). The migrated cell number in sh-NC+ si-NC group was (189.20±16.02), lower than (371.60±38.40) in sh-ACC1+ si-NC group (
P<0.01). The migrated cell number in sh-NC+ si-NC group was higher than that in sh-NC+ si-HSP27 group (152.40±24.30,
P<0.01), and the migrated cell number in sh-ACC1+ si-NC group was higher than that in sh-ACC1+ si-HSP27 group (
P<0.01). The protein expression levels of E-cadherin and Vimentin in sh-NC+ si-NC group were significantly different from those in sh-ACC1+ si-NC and sh-NC+ si-HSP27 groups (
P<0.01). The protein expression levels of E-cadherin and Vimentin in sh-ACC1+ si-NC group were significantly different from those in sh-ACC1+ si-HSP27 group (
P<0.01). After 24 h treatment with C646 at 20 μmmo/L, the migrated cell number in sh-NC+ DMSO group was (190.80±11.95), lower than (395.80±17.10) in sh-ACC1+ DMSO group (
P<0.01). The migrated cell number in sh-NC+ DMSO group was lower than that in sh-NC+ C646 group (256.20±23.32,
P<0.01). The migrated cell number in sh-ACC1+ DMSO group was higher than that in sh-ACC1+ C646 group (87.80±11.23,
P<0.01). The protein expressions of H3K9ac, HSP27, E-cadherin and Vimentin in sh-NC+ DMSO group were significantly different from those in sh-ACC1+ DMSO group and sh-NC+ C646 group (
P<0.01). The protein expression levels of H3K9ac, HSP27, E-cadherin and Vimentin in sh-ACC1+ DMSO group were significantly different from those in sh-ACC1+ C646 group (
P<0.01).
Conclusion:Knockdown of ACC1 promotes the migration of KYSE-450 cell by up-regulating HSP27 and increasing histone acetylation. |
| Author | 钱河 赵宝生 谷城威 刘玉珍 |
| AuthorAffiliation | 新乡医学院第一附属医院胸外科，河南省卫辉市 453100%新乡医学院第一附属医院生命科学研究中心，河南省卫辉市 453100 |
| AuthorAffiliation_xml | – name: 新乡医学院第一附属医院胸外科，河南省卫辉市 453100%新乡医学院第一附属医院生命科学研究中心，河南省卫辉市 453100 |
| Author_FL | Liu Yuzhen Qian He Gu Chengwei Zhao Baosheng |
| Author_FL_xml | – sequence: 1 fullname: Qian He – sequence: 2 fullname: Gu Chengwei – sequence: 3 fullname: Liu Yuzhen – sequence: 4 fullname: Zhao Baosheng |
| Author_xml | – sequence: 1 fullname: 钱河 – sequence: 2 fullname: 谷城威 – sequence: 3 fullname: 刘玉珍 – sequence: 4 fullname: 赵宝生 |
| BookMark | eNotkM1Kw0AcxPdQwVr7GD2m_nc3m02OpfgFBS-9eShJs1FLTcEgQk9KiEJbBA8KtVARDz0pRaEotc3LpO7mLQzoaWZgmB_MBsr5HV8gVMJQptyAreapXW6Vmz7GBDOiESAYGOYaADVpDuWBMKplTWMdFYPgxAFmMq5bmOfR4c_9e7K4Tb6GaTRVyyiNZhW5nKwGD5nD6eWjim-Sz56ahjJ8Tb7vVlFfjfpyuCA8iUMVj9KXJ_n2nH6MZW8m59cqHMvhQMVXcjLfRGue3Q5E8V8LqL6zXa_uabWD3f1qpaYFGEDXBHBHB8GEzgyX4yYhnuUJ27KoYegOt0AIB1PbzQI2TcFdZnrC9JhOXGpZQAuo9Dd7Yfue7R81Wp3zMz8DNrrH3XZ2BgUj49Bfp193Fg |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2B. 4A8 92I 93N PSX TCJ |
| DOI | 10.3760/cma.j.cn112152-20210517-00383 |
| DatabaseName | Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| DocumentTitle_FL | Knockdown of ACC1 promotes migration of esophageal cancer cell |
| EndPage | 489 |
| ExternalDocumentID | zhzl202306004 |
| GrantInformation_xml | – fundername: 河南省医学科技攻关计划省部共建重点项目; 河南省重点科技攻关项目; 新乡市科技攻关计划项目; 新乡医学院第一附属医院青年基金项目; 新乡医学院研究生科研创新支持计划项目; Henan Province Medical Science and Technology Project Research Plan Provincial Co-construction Project; Key Technologies R&D Program of Science and Technology Commission Foundation of Henan Provence; Xinxiang Science and Technology Research Plan; the First Affiliated Hospital of Xinxiang Medical University Youth Foundation; Xinxiang Medical University Graduate Student Innovation Fund funderid: (SBGJ202102188); (202102310103); (GG2020027); (QN－2019－A03); (YJSCX202002Z); (SBGJ202102188); (202102310103); (GG2020027); (QN-2019-A03); (YJSCX202002Z) |
| GroupedDBID | --- -05 123 2B. 4A8 92F 92I 93N ABDBF ABJNI ACGFS ACUHS ALMA_UNASSIGNED_HOLDINGS CCEZO CIEJG CW9 EOJEC OBODZ PSX TCJ TGQ U1G U5O |
| ID | FETCH-LOGICAL-s1004-e07b40e5e456d71c22f9fea993664b790eeb13ad64b188e7d58fe8f542d39903 |
| ISSN | 0253-3766 |
| IngestDate | Thu May 29 04:07:58 EDT 2025 |
| IsPeerReviewed | false |
| IsScholarly | true |
| Issue | 6 |
| Keywords | 乙酰辅酶A羧化酶1 Squamous cell carcinoma Migration 鳞状细胞癌 HSP27 食管肿瘤 Esophageal neoplasms Histone acetylation 组蛋白乙酰化 迁移 热休克蛋白27 ACC1 |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-s1004-e07b40e5e456d71c22f9fea993664b790eeb13ad64b188e7d58fe8f542d39903 |
| PageCount | 8 |
| ParticipantIDs | wanfang_journals_zhzl202306004 |
| PublicationCentury | 2000 |
| PublicationDate | 2023-06-01 |
| PublicationDateYYYYMMDD | 2023-06-01 |
| PublicationDate_xml | – month: 06 year: 2023 text: 2023-06-01 day: 01 |
| PublicationDecade | 2020 |
| PublicationTitle | 中华肿瘤杂志 |
| PublicationTitle_FL | Chinese Journal of Oncology |
| PublicationYear | 2023 |
| Publisher | 新乡医学院第一附属医院胸外科，河南省卫辉市 453100%新乡医学院第一附属医院生命科学研究中心，河南省卫辉市 453100 |
| Publisher_xml | – name: 新乡医学院第一附属医院胸外科，河南省卫辉市 453100%新乡医学院第一附属医院生命科学研究中心，河南省卫辉市 453100 |
| SSID | ssib058574917 ssib051368316 ssib007279245 ssib000995398 ssj0042033 ssib006576341 ssib001103529 |
| Score | 2.4038553 |
| Snippet | 目的:探讨敲低乙酰辅酶A羧化酶1(ACC1)对食管鳞状细胞癌(以下简称食管鳞癌)KYSE－450细胞迁移的影响及分子机制。方法:慢病毒转染法建立对照组细胞系sh－NC和敲低ACC1的细胞... |
| SourceID | wanfang |
| SourceType | Aggregation Database |
| StartPage | 482 |
| Title | 敲低乙酰辅酶A羧化酶1通过上调热休克蛋白27促进食管鳞状细胞癌迁移 |
| URI | https://d.wanfangdata.com.cn/periodical/zhzl202306004 |
| Volume | 45 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVEBS databaseName: EBSCOhost Academic Search Ultimate issn: 0253-3766 databaseCode: ABDBF dateStart: 20150223 customDbUrl: https://search.ebscohost.com/login.aspx?authtype=ip,shib&custid=s3936755&profile=ehost&defaultdb=asn isFulltext: true dateEnd: 99991231 titleUrlDefault: https://search.ebscohost.com/direct.asp?db=asn omitProxy: true ssIdentifier: ssj0042033 providerName: EBSCOhost |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwvV3Na9RAFA-lBfEiioqfpaBz3DqZySQzx6SbpQh6qlDwULLZxCK6gm0vPSmlCm0RPCjUQkU89KQUhaLUdv-Zrbv7X_je2-kmFfELEUKYnXnvzZvfm8y8l52ZOM5V0-A6UTKt8DyXFS9PVMV4UlYSP4WAKxFZQ-F-5xs3_clb3vVpNT00cqW0amlhvj6eLv5wX8nfWBXywK64S_YPLDsQChmQBvvCHSwM99-yMYt9ZhSLBIs9FlWZjilhmDEsNkxDEWexZlGMaZvjhywOMCsMWKyYnmDGH5S5lOLMhMRXYzogkZrpfg5nWiI_3MMqFQG_S4LgipDGRJQIUIuoKvoCasRHIqEcKgklMzWkCkETqjaCnJhkh6AJKQmSfOTSh0UgEhS2qrnEjmRlF9vqi9qBRn1QQIJAFpQARR4BV8VMoEGVBrMxKmKA1kUSADaSRQk1PyLUQHWUq1gIot2CBGrUWAW2AkA3lADa6hEpCi9kjlELVMoDieUXMEIWC8XokSGNfbInWTh0rfEAI5RUZSFZEQAKBeEygZe1HbdF2qMGu4TmgH3ARUADPQr0qFsQvmjfGhoaobeoWHBNQOpPkDEoJ4wOe5yhugj6WKIOGhx7_KeHCfVfmmNxJXu5-HwUzRlUATkcyTBhqNuVeo_ttf-y7aWJVyiJE69f9hL6h57a0bA85Xv9j2dZ79HrfxDre8cEl57BaJLeT8bvjqdNF890ETCWCRfPyKvg__Oy8MgG62QXZxfvCXpLQGcejwhwXfiwMxJG1ahWipKMKp_iCR49hFVFlOIrcCJKUU-Ah4YWiwmUK30tiyhRaRV49Ban79B6gktaqHMIyzGH2SZd-2mDaB9kM0-ad0ou-9RJ54SNtcfC_sB5yhlanD3t3P764kN771n783pvebu7v9xb3gk7-1sHay8h5fYeveq2nrY_rXS3lzpL79pfnh8sr3Y3VjvreyJot5a6rY3e29ed9296Hzc7Kzud3Sfdpc3O-lq39biztXvGmarFUxOTFfuFmcocnpRZyXhQ93imMggjG4GbCpGbPEsgZvN9rx4YnoErK5MG_HC1zoKG0nmmc-WJBgR2XJ51hpsPmtk5ZyzLuUrqUnGepl5Qz-qykYrMz6RKBE-kf94ZtVjM2AlkbuaIdS_8iuCic7wYfS45w_MPF7LLEBDN10dth_gGXAD_iA |
| linkProvider | EBSCOhost |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E6%95%B2%E4%BD%8E%E4%B9%99%E9%85%B0%E8%BE%85%E9%85%B6A%E7%BE%A7%E5%8C%96%E9%85%B61%E9%80%9A%E8%BF%87%E4%B8%8A%E8%B0%83%E7%83%AD%E4%BC%91%E5%85%8B%E8%9B%8B%E7%99%BD27%E4%BF%83%E8%BF%9B%E9%A3%9F%E7%AE%A1%E9%B3%9E%E7%8A%B6%E7%BB%86%E8%83%9E%E7%99%8C%E8%BF%81%E7%A7%BB&rft.jtitle=%E4%B8%AD%E5%8D%8E%E8%82%BF%E7%98%A4%E6%9D%82%E5%BF%97&rft.au=%E9%92%B1%E6%B2%B3&rft.au=%E8%B0%B7%E5%9F%8E%E5%A8%81&rft.au=%E5%88%98%E7%8E%89%E7%8F%8D&rft.au=%E8%B5%B5%E5%AE%9D%E7%94%9F&rft.date=2023-06-01&rft.pub=%E6%96%B0%E4%B9%A1%E5%8C%BB%E5%AD%A6%E9%99%A2%E7%AC%AC%E4%B8%80%E9%99%84%E5%B1%9E%E5%8C%BB%E9%99%A2%E8%83%B8%E5%A4%96%E7%A7%91%EF%BC%8C%E6%B2%B3%E5%8D%97%E7%9C%81%E5%8D%AB%E8%BE%89%E5%B8%82%E3%80%80453100%25%E6%96%B0%E4%B9%A1%E5%8C%BB%E5%AD%A6%E9%99%A2%E7%AC%AC%E4%B8%80%E9%99%84%E5%B1%9E%E5%8C%BB%E9%99%A2%E7%94%9F%E5%91%BD%E7%A7%91%E5%AD%A6%E7%A0%94%E7%A9%B6%E4%B8%AD%E5%BF%83%EF%BC%8C%E6%B2%B3%E5%8D%97%E7%9C%81%E5%8D%AB%E8%BE%89%E5%B8%82%E3%80%80453100&rft.issn=0253-3766&rft.volume=45&rft.issue=6&rft.spage=482&rft.epage=489&rft_id=info:doi/10.3760%2Fcma.j.cn112152-20210517-00383&rft.externalDocID=zhzl202306004 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fzhzl%2Fzhzl.jpg |