锡林郭勒鲜牛乳中乳酸菌分离鉴定及微生物多样性分析

TS252.2; 为完成锡林郭勒少数民族聚集地乳制品中微生物多样性的研究和菌种保护,选取苏尼特左旗和东乌珠穆沁旗6份鲜奶样品进行宏基因组测序分析,并结合传统培养方法进行乳酸菌分离鉴定.结果表明:鲜奶样品共鉴定出微生物菌种165个,优势菌种为乳酸乳球菌(Lactococcus lactis)和肠膜明串珠菌(Leuconostoc mesenteroides).两地鲜奶中共有微生物物种较多,α、β多样性无明显差异,但两组鲜奶中仍存在明显差异物种.苏尼特左旗以乳球菌噬菌体BM13(Lactococcus phage BM13)为主,东乌珠穆沁旗以肠杆菌亚种MGH8(Enterobacter sp.M...

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Published in食品科学 Vol. 44; no. 18; pp. 175 - 182
Main Authors 孙洁, 苏茜, 杜萍, 夏亚男, 赵洁, 于洁, 陈永福
Format Magazine Article
LanguageChinese
Published 内蒙古农业大学乳品生物技术与工程教育部重点实验室,农业农村部奶制品加工重点实验室,内蒙古自治区乳品生物技术与工程重点实验室,内蒙古呼和浩特 010018 2023
Subjects
metagenome
separation and identification
Xilin Gol
锡林郭勒
宏基因组
microbial diversity
微生物多样性
lactic acid bacteria
鲜牛乳
fresh cow milk
乳酸菌
分离鉴定
Online AccessGet full text
ISSN1002-6630
DOI10.7506/spkx1002-6630-20221030-303

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Abstract TS252.2; 为完成锡林郭勒少数民族聚集地乳制品中微生物多样性的研究和菌种保护,选取苏尼特左旗和东乌珠穆沁旗6份鲜奶样品进行宏基因组测序分析,并结合传统培养方法进行乳酸菌分离鉴定.结果表明:鲜奶样品共鉴定出微生物菌种165个,优势菌种为乳酸乳球菌(Lactococcus lactis)和肠膜明串珠菌(Leuconostoc mesenteroides).两地鲜奶中共有微生物物种较多,α、β多样性无明显差异,但两组鲜奶中仍存在明显差异物种.苏尼特左旗以乳球菌噬菌体BM13(Lactococcus phage BM13)为主,东乌珠穆沁旗以肠杆菌亚种MGH8(Enterobacter sp.MGH8)为主.Uniprot数据库注释到1 408 048个基因和412条代谢通路,功能集中在氨基酸代谢与糖酸代谢.从6份鲜奶样品共分离出47株菌,乳酸乳球菌为优势菌种,与宏基因组结果一致.该研究为发酵乳制品的安全生产及发酵剂的开发提供理论依据.
AbstractList TS252.2; 为完成锡林郭勒少数民族聚集地乳制品中微生物多样性的研究和菌种保护,选取苏尼特左旗和东乌珠穆沁旗6份鲜奶样品进行宏基因组测序分析,并结合传统培养方法进行乳酸菌分离鉴定.结果表明:鲜奶样品共鉴定出微生物菌种165个,优势菌种为乳酸乳球菌(Lactococcus lactis)和肠膜明串珠菌(Leuconostoc mesenteroides).两地鲜奶中共有微生物物种较多,α、β多样性无明显差异,但两组鲜奶中仍存在明显差异物种.苏尼特左旗以乳球菌噬菌体BM13(Lactococcus phage BM13)为主,东乌珠穆沁旗以肠杆菌亚种MGH8(Enterobacter sp.MGH8)为主.Uniprot数据库注释到1 408 048个基因和412条代谢通路,功能集中在氨基酸代谢与糖酸代谢.从6份鲜奶样品共分离出47株菌,乳酸乳球菌为优势菌种,与宏基因组结果一致.该研究为发酵乳制品的安全生产及发酵剂的开发提供理论依据.
Author 孙洁
夏亚男
于洁
杜萍
苏茜
赵洁
陈永福
AuthorAffiliation 内蒙古农业大学乳品生物技术与工程教育部重点实验室,农业农村部奶制品加工重点实验室,内蒙古自治区乳品生物技术与工程重点实验室,内蒙古呼和浩特 010018
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Author_FL XIA Yanan
CHEN Yongfu
SUN Jie
SU Qian
ZHAO Jie
DU Ping
YU Jie
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DocumentTitle_FL Isolation and Identification of Lactic Acid Bacteria and Microbial Diversity in Fresh Cow Milk from Xilin Gol
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Issue 18
Keywords metagenome
separation and identification
Xilin Gol
锡林郭勒
宏基因组
microbial diversity
微生物多样性
lactic acid bacteria
鲜牛乳
fresh cow milk
乳酸菌
分离鉴定
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Snippet TS252.2; 为完成锡林郭勒少数民族聚集地乳制品中微生物多样性的研究和菌种保护,选取苏尼特左旗和东乌珠穆沁旗6份鲜奶样品进行宏基因组测序分析,并结合传统培养方法进行乳...
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Title 锡林郭勒鲜牛乳中乳酸菌分离鉴定及微生物多样性分析
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