烟草腺毛优势表达基因NtMYB108b的克隆及分析

Q78; 为揭示NtMYB108转录因子在烟草中的功能,从栽培烟草K326的叶片腺毛中克隆到1条MYB基因的全长cDNA序列,命名为NtMYB108b.NtMYB108b的开放阅读框为828 bp,编码275个氨基酸.生物信息学预测显示,NtMYB108b蛋白定位于细胞核,属于R2R3-MYB型转录因子S20亚组成员,与绒毛状烟草亲缘关系最近,序列相似性为100.00％.实时荧光定量PCR分析显示,NtMYB108b在烟草腺毛中优势表达;NtMYB108b在团棵期叶片中相对表达量最高,在现蕾期5个叶位中的表达无显著差异.经乙烯利(ET)和水杨酸(SA)处理后,NtMYB108b的表达量显著上调...

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Published in烟草科技 Vol. 55; no. 9; pp. 1 - 9
Main Authors 余婧, 赵会纳, 闫筱筱, 郭玉双, 王召军, 余世洲, 王兵, 雷波
Format Journal Article
LanguageChinese
Published 贵州省烟草科学研究院 烟草行业烟草分子遗传重点实验室,贵阳市观山湖区龙滩坝路29号 550081%河南农业大学烟草学院,郑州市文化路95号 450002 01.09.2022
Subjects
腺毛
NtMYB108b
转录因子
烟草
生物信息
Online AccessGet full text
ISSN1002-0861
DOI10.16135/j.issn1002-0861.2022.0093

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Abstract Q78; 为揭示NtMYB108转录因子在烟草中的功能,从栽培烟草K326的叶片腺毛中克隆到1条MYB基因的全长cDNA序列,命名为NtMYB108b.NtMYB108b的开放阅读框为828 bp,编码275个氨基酸.生物信息学预测显示,NtMYB108b蛋白定位于细胞核,属于R2R3-MYB型转录因子S20亚组成员,与绒毛状烟草亲缘关系最近,序列相似性为100.00％.实时荧光定量PCR分析显示,NtMYB108b在烟草腺毛中优势表达;NtMYB108b在团棵期叶片中相对表达量最高,在现蕾期5个叶位中的表达无显著差异.经乙烯利(ET)和水杨酸(SA)处理后,NtMYB108b的表达量显著上调,而经茉莉酸甲酯(MeJA)、赤霉素(GA)和脱落酸(ABA)处理后其表达量无显著变化;使用聚乙二醇(PEG)处理模拟干旱胁迫,处理2 d后NtMYB108b表达量上调,这说明NtMYB108b的功能可能涉及ET、SA信号转导和干旱胁迫.
AbstractList Q78; 为揭示NtMYB108转录因子在烟草中的功能,从栽培烟草K326的叶片腺毛中克隆到1条MYB基因的全长cDNA序列,命名为NtMYB108b.NtMYB108b的开放阅读框为828 bp,编码275个氨基酸.生物信息学预测显示,NtMYB108b蛋白定位于细胞核,属于R2R3-MYB型转录因子S20亚组成员,与绒毛状烟草亲缘关系最近,序列相似性为100.00％.实时荧光定量PCR分析显示,NtMYB108b在烟草腺毛中优势表达;NtMYB108b在团棵期叶片中相对表达量最高,在现蕾期5个叶位中的表达无显著差异.经乙烯利(ET)和水杨酸(SA)处理后,NtMYB108b的表达量显著上调,而经茉莉酸甲酯(MeJA)、赤霉素(GA)和脱落酸(ABA)处理后其表达量无显著变化;使用聚乙二醇(PEG)处理模拟干旱胁迫,处理2 d后NtMYB108b表达量上调,这说明NtMYB108b的功能可能涉及ET、SA信号转导和干旱胁迫.
Author 赵会纳
王兵
王召军
余世洲
余婧
闫筱筱
雷波
郭玉双
AuthorAffiliation 贵州省烟草科学研究院 烟草行业烟草分子遗传重点实验室,贵阳市观山湖区龙滩坝路29号 550081%河南农业大学烟草学院,郑州市文化路95号 450002
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Author_FL YU Jing
YU Shizhou
WANG Bing
LEI Bo
ZHAO Huina
YAN Xiaoxiao
GUO Yushuang
WANG Zhaojun
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DocumentTitle_FL Cloning and analysis of NtMYB108b gene expressed predominantly in tobacco glandular trichomes
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NtMYB108b
转录因子
烟草
生物信息
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Snippet Q78; 为揭示NtMYB108转录因子在烟草中的功能,从栽培烟草K326的叶片腺毛中克隆到1条MYB基因的全长cDNA序列,命名为NtMYB108b.NtMYB108b的开放阅读框为828 bp,编码275个氨基...
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Title 烟草腺毛优势表达基因NtMYB108b的克隆及分析
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