雅致小克银汉霉与芽孢杆菌联合降解义马煤产腐植酸
P618.11; 微生物降解是煤炭清洁高效利用的重要方式之一,但真菌细菌联合培养对煤的降解效果尚无定论.以雅致小克银汉霉(Cunninghamella elegans)和芽孢杆菌(Bacillus sp.)为降解菌,以硝酸氧化的义马煤为底物进行了煤的联合降解实验.利用紫外-可见分光光度计、pH计、电感耦合等离子体质谱仪对降解液的吸光度A450、pH、金属元素(Cr、As、Mn、Pb、Co、Ni、Cu、Zn、Mo)质量浓度进行测定.利用元素分析仪、红外光谱仪、气质联用仪对产物腐植酸进行分析.研究结果显示雅致小克银汉霉(Cunninghamella elegans)、芽孢杆菌(Bacillus s...
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| Published in | 煤炭学报 Vol. 48; no. 11; pp. 4224 - 4232 |
|---|---|
| Main Authors | , , , , , , , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
河南理工大学资源环境学院,河南焦作 454000
01.11.2023
易安蓝焰煤与煤层气共采技术有限责任公司,山西晋城 048000%河南理工大学资源环境学院,河南焦作 454000%煤与煤层气共采国家重点实验室,山西晋城 048000 中国矿业大学煤炭加工与高效洁净利用教育部重点实验室,江苏徐州 221116 煤与煤层气共采国家重点实验室,山西晋城 048000 易安蓝焰煤与煤层气共采技术有限责任公司,山西晋城 048000%中国矿业大学化工学院,江苏徐州 221116 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0253-9993 |
| DOI | 10.13225/j.cnki.jccs.2022.1581 |
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| Abstract | P618.11; 微生物降解是煤炭清洁高效利用的重要方式之一,但真菌细菌联合培养对煤的降解效果尚无定论.以雅致小克银汉霉(Cunninghamella elegans)和芽孢杆菌(Bacillus sp.)为降解菌,以硝酸氧化的义马煤为底物进行了煤的联合降解实验.利用紫外-可见分光光度计、pH计、电感耦合等离子体质谱仪对降解液的吸光度A450、pH、金属元素(Cr、As、Mn、Pb、Co、Ni、Cu、Zn、Mo)质量浓度进行测定.利用元素分析仪、红外光谱仪、气质联用仪对产物腐植酸进行分析.研究结果显示雅致小克银汉霉(Cunninghamella elegans)、芽孢杆菌(Bacillus sp.)、混合菌的腐植酸产率分别为 58.17%、61.00%、67.17%,混合菌降解液的pH与细菌接近,混合菌降解的腐植酸样品中检出细菌的特征性产物而真菌的特征性产物则未检出,说明两株菌联合强化了碱性环境,提高了硝酸氧化煤的降解率,降解过程中细菌起主导作用.金属元素(Cr、As、Mn、Pb、Co、Ni、Cu、Zn、Mo)在降解过程中从煤迁移到了降解液,其中Cr、As、Pb、Ni、Cu、Mo的质量浓度与A450 拟合的判定系数(R2)大于 0.6,说明降解液中这 6种金属元素的质量浓度可表征降解率的相对大小.化学提取腐植酸与生物提取腐植酸均富含羧基、羟基、羰基等活性官能团、长链脂肪酸(C16、C18)和4种吡咯衍生物,生物提取腐植酸还含有分子量较小的脂肪酸(C3、C4、C5、C13、C14、C15)、2种吡咯衍生物和呋喃等含氮化合物,生物提取腐植酸的C、H元素质量分数高于化学提取腐植酸. |
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| AbstractList | P618.11; 微生物降解是煤炭清洁高效利用的重要方式之一,但真菌细菌联合培养对煤的降解效果尚无定论.以雅致小克银汉霉(Cunninghamella elegans)和芽孢杆菌(Bacillus sp.)为降解菌,以硝酸氧化的义马煤为底物进行了煤的联合降解实验.利用紫外-可见分光光度计、pH计、电感耦合等离子体质谱仪对降解液的吸光度A450、pH、金属元素(Cr、As、Mn、Pb、Co、Ni、Cu、Zn、Mo)质量浓度进行测定.利用元素分析仪、红外光谱仪、气质联用仪对产物腐植酸进行分析.研究结果显示雅致小克银汉霉(Cunninghamella elegans)、芽孢杆菌(Bacillus sp.)、混合菌的腐植酸产率分别为 58.17%、61.00%、67.17%,混合菌降解液的pH与细菌接近,混合菌降解的腐植酸样品中检出细菌的特征性产物而真菌的特征性产物则未检出,说明两株菌联合强化了碱性环境,提高了硝酸氧化煤的降解率,降解过程中细菌起主导作用.金属元素(Cr、As、Mn、Pb、Co、Ni、Cu、Zn、Mo)在降解过程中从煤迁移到了降解液,其中Cr、As、Pb、Ni、Cu、Mo的质量浓度与A450 拟合的判定系数(R2)大于 0.6,说明降解液中这 6种金属元素的质量浓度可表征降解率的相对大小.化学提取腐植酸与生物提取腐植酸均富含羧基、羟基、羰基等活性官能团、长链脂肪酸(C16、C18)和4种吡咯衍生物,生物提取腐植酸还含有分子量较小的脂肪酸(C3、C4、C5、C13、C14、C15)、2种吡咯衍生物和呋喃等含氮化合物,生物提取腐植酸的C、H元素质量分数高于化学提取腐植酸. |
| Abstract_FL | Biodegradation is one of the important ways for the clean and efficient utilization of coal.However,the effect-iveness of degradation by the combination of fungi and bacteria has not been well understood.In the present study,the combined degradation of the Yima coal was tested.The coal samples were firstly oxidized with nitric acid,followed by cultured in the media of Cunninghamella elegans and Bacillus sp..The absorbance of A450,pH and metallic element(Cr,As,Mn,Pb,Co,Ni,Cu,Zn,Mo)contents of the degradation solution were determined by UV-visible spectrophotometry,pH meter and inductively coupled plasma mass spectrometry,respectively.The humic acid was analyzed by element ana-lyzer,Fourier transform infrared spectroscopy and gas chromatog-raphy-mass spectrometry.The results showed that the humic acid yields of C.elegans,Bacillus sp.and their mixture were 58.17%,61.00%and 67.17%,respectively.The pH of the degradation solution of mixed strains was similar to that of the bacteria.The characteristic products of the bacteria de-gradation were detected in the humic acid samples derived from mixed strains,while the opposite was true for the fungi.It was suggested that the combination of the two strains enhanced the alkaline environment and improved the degradation rate of nitric acid-treated coal.The bacteria played a leading role in the degradation process.Metallic elements(Cr,As,Mn,Pb,Co,Ni,Cu,Zn,Mo)were transferred from coal to the degradation solution during the degradation process,and the contents of Cr,As,Pb,Ni,Cu and Mo were fitted with A450,the coefficient of determination(R2)were greater than 0.6.It indicated that the contents of these six metal elements in the degradation solution could represent the degradation rate.Chemically extracted humic acid and biologically extracted humic acid were rich in the active functional groups such as carboxyl,hydroxyl,carbonyl,long-chain fatty acids(C16,C18)and four pyrrole derivatives.The biologically extracted humic acid also contained fatty acids(C3,C4,C5,C13,C14,C15),of smaller molecular weight,as well as nitrogen-con-taining compounds such as two pyrrole derivatives and a furan.The contents of C and H elements in the biologically ex-tracted humic acid were higher than that in the chemically extracted humic acid. |
| Author | 何环 赵晗 李国富 牛江露 赵娜 陈林勇 崔宇翔 关嘉栋 宋燕莉 任恒星 王保玉 刘建民 |
| AuthorAffiliation | 河南理工大学资源环境学院,河南焦作 454000;煤与煤层气共采国家重点实验室,山西晋城 048000;易安蓝焰煤与煤层气共采技术有限责任公司,山西晋城 048000%河南理工大学资源环境学院,河南焦作 454000%煤与煤层气共采国家重点实验室,山西晋城 048000;易安蓝焰煤与煤层气共采技术有限责任公司,山西晋城 048000%中国矿业大学化工学院,江苏徐州 221116;中国矿业大学煤炭加工与高效洁净利用教育部重点实验室,江苏徐州 221116 |
| AuthorAffiliation_xml | – name: 河南理工大学资源环境学院,河南焦作 454000;煤与煤层气共采国家重点实验室,山西晋城 048000;易安蓝焰煤与煤层气共采技术有限责任公司,山西晋城 048000%河南理工大学资源环境学院,河南焦作 454000%煤与煤层气共采国家重点实验室,山西晋城 048000;易安蓝焰煤与煤层气共采技术有限责任公司,山西晋城 048000%中国矿业大学化工学院,江苏徐州 221116;中国矿业大学煤炭加工与高效洁净利用教育部重点实验室,江苏徐州 221116 |
| Author_FL | CUI Yuxiang ZHAO Na CHEN Linyong LIU Jianmin LI Guofu GUAN Jiadong REN Hengxing WANG Baoyu HE Huan SONG Yanli NIU Jianglu ZHAO Han |
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| DocumentTitle_FL | Humic acid production from the degradation of Yima coal by Cunninghamella elegans combined with Bacillus sp |
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| Keywords | 微生物降解 Bacillus sp 金属元素 Yima coal 义马煤 metallic elements 芽孢杆菌 雅致小克银汉霉 biodegradation of coal Cunninghamella elegans |
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| Publisher | 河南理工大学资源环境学院,河南焦作 454000 易安蓝焰煤与煤层气共采技术有限责任公司,山西晋城 048000%河南理工大学资源环境学院,河南焦作 454000%煤与煤层气共采国家重点实验室,山西晋城 048000 中国矿业大学煤炭加工与高效洁净利用教育部重点实验室,江苏徐州 221116 煤与煤层气共采国家重点实验室,山西晋城 048000 易安蓝焰煤与煤层气共采技术有限责任公司,山西晋城 048000%中国矿业大学化工学院,江苏徐州 221116 |
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