大鼠感染泡球蚴后外周血及脾脏中滤泡辅助T淋巴细胞的变化
目的 分析泡球蚴感染宿主后外周血和脾脏中滤泡辅助T淋巴细胞(Tfh)水平与包虫病进展的关系。 方法 20只SD大鼠随机分为正常对照组和模型组，每组10只，模型组开腹直视下在右肝接种约2000个原头节，对照组不做任何处理，3个月后麻醉处死SD大鼠，取外周血和脾脏细胞，以CD4+CXCR5+PD1+为Tfh细胞的标记，使用流式细胞术检测外周血和脾脏中Tfh细胞的水平。使用t检验比较两组之间Tfh细胞的差异。 结果 大鼠感染泡球蚴3个月后，肝脏可见明显病灶，HE染色显示病灶中可见原头节。泡球蚴感染模型组外周血中CD4+CXCR5+PD1+Tfh细胞在CD4+细胞中的平均占比为25.63%±3.47%...
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| Published in | Linchuang gandanbing zazhi Vol. 37; no. 7; pp. 1657 - 1661 |
|---|---|
| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
Changchun
Journal of Clinical Hepatology
01.07.2021
青海大学临床医学院,西宁810000 青海大学附属医院中心实验室,西宁810000 青海省包虫病研究重点实验室,西宁810000%青海大学附属医院中心实验室,西宁810000%青海大学附属医院中心实验室,西宁810000 |
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| Online Access | Get full text |
| ISSN | 1001-5256 2097-3497 |
| DOI | 10.3969/j.issn.1001-5256.2021.07.035 |
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| Abstract | 目的 分析泡球蚴感染宿主后外周血和脾脏中滤泡辅助T淋巴细胞(Tfh)水平与包虫病进展的关系。 方法 20只SD大鼠随机分为正常对照组和模型组，每组10只，模型组开腹直视下在右肝接种约2000个原头节，对照组不做任何处理，3个月后麻醉处死SD大鼠，取外周血和脾脏细胞，以CD4+CXCR5+PD1+为Tfh细胞的标记，使用流式细胞术检测外周血和脾脏中Tfh细胞的水平。使用t检验比较两组之间Tfh细胞的差异。 结果 大鼠感染泡球蚴3个月后，肝脏可见明显病灶，HE染色显示病灶中可见原头节。泡球蚴感染模型组外周血中CD4+CXCR5+PD1+Tfh细胞在CD4+细胞中的平均占比为25.63%±3.47%，正常对照组为11.12%±2.94%，2组比较差异有统计学意义(t=10.230，P＜0.001)，模型组CD4+CXCR5+PD1+Tfh细胞在所有细胞中所占的比例较正常对照组下降显著(0.08%±0.02% vs 0.18%±0.05%, t=5.520，P＜0.001)；在所有细胞中，模型组脾脏中CD4+CXCR5+PD1+Tfh细胞所占的比例(3.00%±0.42%)显著低于正常对照组(5.30%±1.40%)(t=4.769，P＜0.001)。 结论 外周血中Tfh细胞与包虫病进展密切相关，有望成为反应泡球蚴感染的指标。 |
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| AbstractList | R532.32; 目的 分析泡球蚴感染宿主后外周血和脾脏中滤泡辅助T淋巴细胞(Tfh)水平与包虫病进展的关系.方法 20只SD大鼠随机分为正常对照组和模型组,每组10只,模型组开腹直视下在右肝接种约2000个原头节,对照组不做任何处理,3个月后麻醉处死SD大鼠,取外周血和脾脏细胞,以CD4+CXCR5+PD1+为Tfh细胞的标记,使用流式细胞术检测外周血和脾脏中Tfh细胞的水平.使用t检验比较两组之间Tfh细胞的差异.结果 大鼠感染泡球蚴3个月后,肝脏可见明显病灶,HE染色显示病灶中可见原头节.泡球蚴感染模型组外周血中CD4+CXCR5+PD1+Tfh细胞在CD4+细胞中的平均占比为25.63％ ±3.47％,正常对照组为11.12％ ±2.94％,2组比较差异有统计学意义(t=10.230,P<0.001),模型组CD4+CXCR5+PD1+Tfh细胞在所有细胞中所占的比例较正常对照组下降显著(0.08％ ±0.02％vs 0.18％ ±0.05％,t=5.520,P<0.001);在所有细胞中,模型组脾脏中CD4+CXCR5+PD1+Tfh细胞所占的比例(3.00％ ±0.42％)显著低于正常对照组(5.30％ ±1.40％)(t=4.769,P<0.001).结论 外周血中Tfh细胞与包虫病进展密切相关,有望成为反应泡球蚴感染的指标. 目的 分析泡球蚴感染宿主后外周血和脾脏中滤泡辅助T淋巴细胞(Tfh)水平与包虫病进展的关系。 方法 20只SD大鼠随机分为正常对照组和模型组，每组10只，模型组开腹直视下在右肝接种约2000个原头节，对照组不做任何处理，3个月后麻醉处死SD大鼠，取外周血和脾脏细胞，以CD4+CXCR5+PD1+为Tfh细胞的标记，使用流式细胞术检测外周血和脾脏中Tfh细胞的水平。使用t检验比较两组之间Tfh细胞的差异。 结果 大鼠感染泡球蚴3个月后，肝脏可见明显病灶，HE染色显示病灶中可见原头节。泡球蚴感染模型组外周血中CD4+CXCR5+PD1+Tfh细胞在CD4+细胞中的平均占比为25.63%±3.47%，正常对照组为11.12%±2.94%，2组比较差异有统计学意义(t=10.230，P＜0.001)，模型组CD4+CXCR5+PD1+Tfh细胞在所有细胞中所占的比例较正常对照组下降显著(0.08%±0.02% vs 0.18%±0.05%, t=5.520，P＜0.001)；在所有细胞中，模型组脾脏中CD4+CXCR5+PD1+Tfh细胞所占的比例(3.00%±0.42%)显著低于正常对照组(5.30%±1.40%)(t=4.769，P＜0.001)。 结论 外周血中Tfh细胞与包虫病进展密切相关，有望成为反应泡球蚴感染的指标。 |
| Author | 侯静 张涛 黄登亮 田美媛 孙莉 江源 李建华 张耀刚 马艳艳 樊海宁 |
| AuthorAffiliation | 青海大学附属医院中心实验室,西宁810000;青海省包虫病研究重点实验室,西宁810000%青海大学附属医院中心实验室,西宁810000%青海大学附属医院中心实验室,西宁810000;青海大学临床医学院,西宁810000 |
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| Author_FL | ZHANG Yaogang HUANG Dengliang MA Yan-yan SUN Li ZHANG Tao FAN Haining JIANG Yuan HOU Jing TIAN Meiyuan LI Jianhua |
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| Snippet | 目的 分析泡球蚴感染宿主后外周血和脾脏中滤泡辅助T淋巴细胞(Tfh)水平与包虫病进展的关系。 方法 20只SD大鼠随机分为正常对照组和模型组，每组10只，模型组开腹直视下在右... R532.32; 目的 分析泡球蚴感染宿主后外周血和脾脏中滤泡辅助T淋巴细胞(Tfh)水平与包虫病进展的关系.方法 20只SD大鼠随机分为正常对照组和模型组,每组10只,模型组开腹直视... |
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