辅助性T淋巴细胞9在慢性丙型肝炎初治患者中的变化及临床意义
摘要:目的观察辅助性T淋巴细胞9 (Th9) 、IL-9及相关转录因子在慢性丙型肝炎初治患者中的变化,评估与临床指标的相关性。方法选取2018年12月-2019年7月于海南省人民医院就诊的慢性丙型肝炎初治患者29例和健康对照者15例,慢性丙型肝炎患者接受索磷布韦/维帕他韦抗病毒治疗12周,分离血浆和外周血单个核细胞 (PBMC) 。流式细胞术检测PBMC中CD3+CD4+IL-9+的Th9比例,ELISA检测血浆中IL-9水平,实时定量PCR法检测PBMC中IL-9和转录因子PU. 1、Foxo1的mRNA相对表达量。两组间比较采用t检验或配对t检验,采用Pearson检验进行相关性分析。结果...
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| Published in | Linchuang gandanbing zazhi Vol. 36; no. 12; pp. 2705 - 2708 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
Changchun
Journal of Clinical Hepatology
01.12.2020
海南省人民医院,海南医学院附属海南医院 感染科,海口570311%海南省人民医院,海南医学院附属海南医院 消化内科,海口570311%博鳌超级医院感染科,海南琼海571435 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-5256 2097-3497 |
| DOI | 10.3969/j.issn.1001-5256.2020.12.013 |
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| Abstract | 摘要:目的观察辅助性T淋巴细胞9 (Th9) 、IL-9及相关转录因子在慢性丙型肝炎初治患者中的变化,评估与临床指标的相关性。方法选取2018年12月-2019年7月于海南省人民医院就诊的慢性丙型肝炎初治患者29例和健康对照者15例,慢性丙型肝炎患者接受索磷布韦/维帕他韦抗病毒治疗12周,分离血浆和外周血单个核细胞 (PBMC) 。流式细胞术检测PBMC中CD3+CD4+IL-9+的Th9比例,ELISA检测血浆中IL-9水平,实时定量PCR法检测PBMC中IL-9和转录因子PU. 1、Foxo1的mRNA相对表达量。两组间比较采用t检验或配对t检验,采用Pearson检验进行相关性分析。结果慢性丙型肝炎初治患者外周血Th9比例明显低于健康对照者 (0. 92%±0. 14%vs 1. 14%±0. 21%,t=4. 31,P<0. 001) ,血浆IL-9水平亦明显低于健康对照者[ (248. 27±66. 97) pg/ml vs (309. 02±88. 48) pg/ml,t=2. 63,P=0. 012]。慢性丙型肝炎初治患者PBMC中IL-9和PU. 1 mRNA相对表达量均明显低于健... |
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| AbstractList | R512.63; 目的 观察辅助性T淋巴细胞9(Th9)、IL-9及相关转录因子在慢性丙型肝炎初治患者中的变化,评估与临床指标的相关性.方法 选取2018年12月-2019年7月于海南省人民医院就诊的慢性丙型肝炎初治患者29例和健康对照者15例,慢性丙型肝炎患者接受索磷布韦/维帕他韦抗病毒治疗12周,分离血浆和外周血单个核细胞(PBMC).流式细胞术检测PBMC中CD3+CD4+IL-9+的Th9比例,ELISA检测血浆中IL-9水平,实时定量PCR法检测PBMC中IL-9和转录因子PU.1、Foxo1的mRNA相对表达量.两组间比较采用t检验或配对t检验,采用Pearson检验进行相关性分析.结果 慢性丙型肝炎初治患者外周血Th9比例明显低于健康对照者(0.92％ ±0.14％vs 1.14％ ±0.21％,t=4.31,P<0.001),血浆IL-9水平亦明显低于健康对照者[(248.27±66.97)pg/ml vs(309.02±88.48)pg/ml,t=2.63,P=0.012].慢性丙型肝炎初治患者PBMC中IL-9和PU.1 mRNA相对表达量均明显低于健康对照者(t值分别为20.67、23.21,P值均<0.001),但Foxo1 mRNA相对表达量在慢性丙型肝炎初治患者和健康对照者之间的差异无统计学意义(t=0.704,P>0.05).慢性丙型肝炎初治患者外周血Th9比例、IL-9水平、IL-9 mRNA和PU.1 mRNA与HCV RNA呈负相关(r值分别为-0.46、-0.38、-0.52、-0.41,P值均<0.05),但与ALT水平无明显相关性(P值均>0.05).索磷布韦/维帕他韦抗病毒治疗使29例慢性丙型肝炎患者均达到病毒学应答,抗病毒治疗后外周血Th9比例和PU.1 mRNA均明显高于基线(t值分别为2.20、6.52,P值均<0.05),但血浆IL-9水平和IL-9 mRNA相对表达量在基线和治疗后差异均无统计学意义(P值均>0.05).结论 慢性HCV感染可能抑制Th9活化,Th9可能参与了HCV感染慢性化. 摘要:目的观察辅助性T淋巴细胞9 (Th9) 、IL-9及相关转录因子在慢性丙型肝炎初治患者中的变化,评估与临床指标的相关性。方法选取2018年12月-2019年7月于海南省人民医院就诊的慢性丙型肝炎初治患者29例和健康对照者15例,慢性丙型肝炎患者接受索磷布韦/维帕他韦抗病毒治疗12周,分离血浆和外周血单个核细胞 (PBMC) 。流式细胞术检测PBMC中CD3+CD4+IL-9+的Th9比例,ELISA检测血浆中IL-9水平,实时定量PCR法检测PBMC中IL-9和转录因子PU. 1、Foxo1的mRNA相对表达量。两组间比较采用t检验或配对t检验,采用Pearson检验进行相关性分析。结果慢性丙型肝炎初治患者外周血Th9比例明显低于健康对照者 (0. 92%±0. 14%vs 1. 14%±0. 21%,t=4. 31,P<0. 001) ,血浆IL-9水平亦明显低于健康对照者[ (248. 27±66. 97) pg/ml vs (309. 02±88. 48) pg/ml,t=2. 63,P=0. 012]。慢性丙型肝炎初治患者PBMC中IL-9和PU. 1 mRNA相对表达量均明显低于健... |
| Author | 梁志军 田元元 王姣 |
| AuthorAffiliation | 海南省人民医院,海南医学院附属海南医院 感染科,海口570311%海南省人民医院,海南医学院附属海南医院 消化内科,海口570311%博鳌超级医院感染科,海南琼海571435 |
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| Author_FL | TIAN Yuanyuan WANG Jiao LIANG Zhijun |
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| Copyright | 2020. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| Keywords | CD4阳性T淋巴细胞 白细胞介素9 丙型肝炎,慢性 |
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| Publisher | Journal of Clinical Hepatology 海南省人民医院,海南医学院附属海南医院 感染科,海口570311%海南省人民医院,海南医学院附属海南医院 消化内科,海口570311%博鳌超级医院感染科,海南琼海571435 |
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| Snippet | 摘要:目的观察辅助性T淋巴细胞9 (Th9) 、IL-9及相关转录因子在慢性丙型肝炎初治患者中的变化,评估与临床指标的相关性。方法选取2018年12月-2019年7月于海南省人民医院就诊的... R512.63; 目的 观察辅助性T淋巴细胞9(Th9)、IL-9及相关转录因子在慢性丙型肝炎初治患者中的变化,评估与临床指标的相关性.方法 选取2018年12月-2019年7月于海南省人民医院就... |
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| Title | 辅助性T淋巴细胞9在慢性丙型肝炎初治患者中的变化及临床意义 |
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