肝细胞移植治疗肝硬化合并小肝综合征大鼠模型的效果观察

摘要:目的观察肝细胞移植对大鼠肝硬化合并小肝综合征的治疗效果。方法对40只雄性SD大鼠用四氯化碳皮下注射加乙醇饮用建立肝硬化大鼠模型。从肝硬化大鼠模型中随机抽取30只,分为A组 (术前3 d进行肝细胞移植) 、B组 (手术时行肝细胞移植) 、C组 (不进行肝细胞移植处理) ,每组10只,进行70%肝脏切除,术后观察各组大鼠生存率、不同时点血生化以及肝脏HE染色病理切片变化情况。符合正态分布的计量资料多组间比较采用方差分析,进一步两组间比较,方差齐采用LSD-t检验,方差不齐采用Games-Howell检验。生存率比较采用log-rank检验。结果 A组术后存活率 (90%,9/10) 明显高于...

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Published inLinchuang gandanbing zazhi Vol. 36; no. 5; pp. 1038 - 1042
Main Authors 贾磊, 温欣慰, 李海洋
Format Journal Article
LanguageChinese
Published Changchun Journal of Clinical Hepatology 01.05.2020
贵州医科大学,贵阳,550004%贵州医科大学,贵阳550004
贵州医科大学附属医院肝胆外科,贵阳550004
Subjects
Liver cirrhosis
Surgery
小肝综合征
细胞移植
肝硬化
Online AccessGet full text
ISSN1001-5256
2097-3497
DOI10.3969/j.issn.1001-5256.2020.05.018

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Abstract 摘要:目的观察肝细胞移植对大鼠肝硬化合并小肝综合征的治疗效果。方法对40只雄性SD大鼠用四氯化碳皮下注射加乙醇饮用建立肝硬化大鼠模型。从肝硬化大鼠模型中随机抽取30只,分为A组 (术前3 d进行肝细胞移植) 、B组 (手术时行肝细胞移植) 、C组 (不进行肝细胞移植处理) ,每组10只,进行70%肝脏切除,术后观察各组大鼠生存率、不同时点血生化以及肝脏HE染色病理切片变化情况。符合正态分布的计量资料多组间比较采用方差分析,进一步两组间比较,方差齐采用LSD-t检验,方差不齐采用Games-Howell检验。生存率比较采用log-rank检验。结果 A组术后存活率 (90%,9/10) 明显高于B组 (50%,5/10) 和C组 (30%,3/10) (χ2=6.440,P=0. 04) 。术后24 h A、B、C 3组ALT平均值分别为 (860. 1±39. 9) U/L、 (904. 6±35. 6) U/L、 (927. 4±17. 0) U/L (F=6.808,P=0. 007) ,AST平均值分别为 (896. 3±44. 7) U/L、 (923. 8±31. 6) U/L、 (950. 0±16. 3) U...
AbstractList R575; 目的 观察肝细胞移植对大鼠肝硬化合并小肝综合征的治疗效果.方法 对40只雄性SD大鼠用四氯化碳皮下注射加乙醇饮用建立肝硬化大鼠模型.从肝硬化大鼠模型中随机抽取30只,分为A组(术前3 d进行肝细胞移植)、B组(手术时行肝细胞移植)、C组(不进行肝细胞移植处理),每组10只,进行70％肝脏切除,术后观察各组大鼠生存率、不同时点血生化以及肝脏HE染色病理切片变化情况.符合正态分布的计量资料多组间比较采用方差分析,进一步两组间比较,方差齐采用LSD-t检验,方差不齐采用Games-Howell检验.生存率比较采用log-rank检验.结果 A组术后存活率(90％,9/10)明显高于B组(50％,5/10)和C组(30％,3/10)(χ2=6.440,P=0.04).术后24 h A、B、C 3组ALT平均值分别为(860.1±39.9)U/L、(904.6±35.6)U/L、(927.4±17.0)U/L(F=6.808,P=0.007),AST平均值分别为(896.3±44.7)U/L、(923.8±31.6)U/L、(950.0±16.3)U/L(F=3.666,P=0.047),Alb平均值分别为(25.6±0.5)g/L、(24.8±0.6)g/L、(23.4±0.4)g/L(F=26.577,P<0.001),A组的恢复情况均有好于B、C两组的趋势,但差异不全有统计学意义.至术后240 h 3组ALT平均值分别为(97.8±10.0)U/L、(128.2±20.7)U/L、(150.5±14.8)U/L(F=13.816,P=0.001),AST平均值分别为(108.7±10.8)U/L、(142.0±14.1)U/L、(161.0±21.2)U/L(F=17.220,P<0.001),Alb平均值分别为(29.1±0.6)g/L、(28.0±0.2)g/L、(27.0±0.2)g/L(F=15.629,P=0.001).术后72 h肝组织病理学检查结果 显示,C组大鼠肝索结构消失,大块肝细胞坏死及炎性细胞浸润,A组仅见肝细胞气球样病变,点状细胞坏死,少量炎性细胞浸润.结论 肝细胞移植可有效促进肝硬化大鼠术后小肝综合征肝功能恢复及提高生存率,且治疗效果可能与细胞移植的时间点有关.
摘要:目的观察肝细胞移植对大鼠肝硬化合并小肝综合征的治疗效果。方法对40只雄性SD大鼠用四氯化碳皮下注射加乙醇饮用建立肝硬化大鼠模型。从肝硬化大鼠模型中随机抽取30只,分为A组 (术前3 d进行肝细胞移植) 、B组 (手术时行肝细胞移植) 、C组 (不进行肝细胞移植处理) ,每组10只,进行70%肝脏切除,术后观察各组大鼠生存率、不同时点血生化以及肝脏HE染色病理切片变化情况。符合正态分布的计量资料多组间比较采用方差分析,进一步两组间比较,方差齐采用LSD-t检验,方差不齐采用Games-Howell检验。生存率比较采用log-rank检验。结果 A组术后存活率 (90%,9/10) 明显高于B组 (50%,5/10) 和C组 (30%,3/10) (χ2=6.440,P=0. 04) 。术后24 h A、B、C 3组ALT平均值分别为 (860. 1±39. 9) U/L、 (904. 6±35. 6) U/L、 (927. 4±17. 0) U/L (F=6.808,P=0. 007) ,AST平均值分别为 (896. 3±44. 7) U/L、 (923. 8±31. 6) U/L、 (950. 0±16. 3) U...
Author 温欣慰
贾磊
李海洋
AuthorAffiliation 贵州医科大学,贵阳,550004%贵州医科大学,贵阳550004;贵州医科大学附属医院肝胆外科,贵阳550004
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WEN Xinwei
LI Haiyang
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PublicationTitle_FL Journal of Clinical Hepatology
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贵州医科大学,贵阳,550004%贵州医科大学,贵阳550004
贵州医科大学附属医院肝胆外科,贵阳550004
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Snippet 摘要:目的观察肝细胞移植对大鼠肝硬化合并小肝综合征的治疗效果。方法对40只雄性SD大鼠用四氯化碳皮下注射加乙醇饮用建立肝硬化大鼠模型。从肝硬化大鼠模型中随机抽取30只,...
R575; 目的 观察肝细胞移植对大鼠肝硬化合并小肝综合征的治疗效果.方法 对40只雄性SD大鼠用四氯化碳皮下注射加乙醇饮用建立肝硬化大鼠模型.从肝硬化大鼠模型中随机抽取30...
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SubjectTerms Liver cirrhosis
Surgery
Title 肝细胞移植治疗肝硬化合并小肝综合征大鼠模型的效果观察
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