Endothelial Nitric Oxide Synthase T-786C and G-894T Gene Polymorphisms: A Risk Assessment of Coronary Heart Disease

• Published in: Journal of the Association of Physicians of India Vol. 71; no. 9; p. 45
• Main Authors: Jaishankar, Thirunavukkarasu, Shivasekar, Meera, Vinodhini, V M
• Format: Journal Article
• Language: English
• Published: India 01.09.2023
• Subjects: Adult; Case-Control Studies; Coronary Disease - epidemiology; Coronary Disease - genetics; Cross-Sectional Studies; Female; Genetic Predisposition to Disease; Humans; India - epidemiology; Male; Middle Aged; Nitric Oxide - metabolism; Nitric Oxide Synthase Type III - genetics; Polymorphism, Genetic; Risk Assessment - methods; India
• ISSN: 0004-5772
• DOI: 10.59556/japi.71.0294

The polymorphism of the endothelial nitric oxide synthase (eNOS) gene is thought to enhance the risk of coronary heart disease (CHD). The most protruding reason for endothelial dysfunction is the diminished production of NO. In the eNOS encoding gene, a number of mutations were associated with decreased NO effect promoting the presence of CHD. This study aims to look at the role of polymorphisms in the eNOS genes G-894T and T-786C in young South Indians. From January 2022 to May 2022, 91 angiographically proven CHD subjects attending the Department of Cardiology and Medicine and 91 controls from a master health checkup in the age group of 45 years participated in this observational cross-sectional study at SRM Medical College Hospital and Research Centre in Chennai, Tamil Nadu, India. Overnight fasting plasma samples were taken for analysis of the lipid profile as well as NO by Griess reaction utilizing an enzyme-linked immunosorbent assay (ELISA) technique. Polymerase chain reaction (PCR) and restricted fragment length polymorphism (RFLP) were used to amplify the T-786C and G-894T eNOS genes. When compared to controls, the mean level of serum NO in CHD patients was considerably lower. For eNOS T-786C polymorphism, the distribution of TC genotype ( = 0.017), odds (OD) ratio = 2.1, CC genotype ( = 0.011), OD ratio = 3.75, and minor C allele frequency ( = 0.001). And for eNOS G-894T polymorphism, the distribution of GT genotype ( = 0.01), TT genotype ( =0.02) with OD ratio and minor T allele frequency ( = 0.002) with OD ratio = 1.07 and 0.83. Our study concludes that the polymorphism of eNOS T-786C and G-894T genes may be the main causative association for the presence of CHD. How to cite this article: Jaishankar T, Shivasekar M, Vinodhini VM. Endothelial Nitric Oxide Synthase T-786C and G-894T Gene Polymorphisms: A Risk Assessment of Coronary Heart Disease. J Assoc Physicians India 2023;71(9):45-50.