팔미트 지방산으로 자극된 혈관내피세포에서 대황 및 목단피가염증 및 인슐린 저항성에 미치는 효과

• Published in: 생약학회지(Korean Journal of Pharmacognosy) Vol. 45; no. 1; pp. 28 - 34
• Main Authors: 이준서(Joon Suh Lee), 이재철(Jae Cheol Lee), 윤용갑(Yong Gab Yun)
• Format: Journal Article
• Language: Korean
• Published: 한국생약학회 01.03.2014
• Subjects: 약학; Insulin resistance; Palmitic acid; Anti-inflammation; Rhei Rhizoma; Moutan cortex
• ISSN: 0253-3073; 2288-9299

Rhei Rhizoma (RR) and Moutan cortex (MC) have been reported to have anti-inflammatory effects. However, littleis known about the effects of RR and MC on endothelial inflammation and insulin resistance (IR). This study aims to investigatewhether the water extracts of RR and MC could exert protection against palmitic acid (PA)-induced inflammation and IR inhuman umbilical vein endothelial cells (HUVECs). HUVECs were pretreated for 6 h with RR or MC, and then exposed to PAfor 24 h. The levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were determined by enzyme-linked immunosorbantassay kits. Western blot analysis was performed for activation of nuclear factor-κB (NF-κB) and insulin receptor substrate-1 (IRS-1). In HUVECs stimulated with PA, both RR and MC significantly inhibited the production of TNF-α and IL-6 and the activation of NF-κB. At the same concentrations, the inhibitory effects of RR were more potent than those of MC. PA reduced insulin-induced phosphorylation of IRS-1, which was reversed by RR and MC. The results suggest that RR andMC are effective in inhibiting PA-associated endothelial inflammation and ameliorating IR by beneficial regulation of NF-κBand IRS-1 activation. KCI Citation Count: 0